CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/161.mrc Output diagnostic file name Micrographs_0601/161.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/161.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2346.000 8284.000 Mean, RMS : 4537.265 219.1042 TITLE 1: emi2mrc: 161.emi Sun Jan 05 16:53:12 2014 TITLE 2: kV 300, Mag 78000x, Def 23790, Spot 4, Int 55.10%, Obj 88.02%, Dif 73.67% TITLE 3: X 13.361, Y 320.264, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00595 10200.00 10000.00 0.00 -0.00588 11200.00 10000.00 0.00 -0.00580 11400.00 10000.00 0.00 -0.00560 11600.00 10000.00 0.00 -0.00529 11800.00 10000.00 0.00 -0.00485 12000.00 10000.00 0.00 -0.00456 12200.00 10000.00 0.00 -0.00423 12400.00 10000.00 0.00 -0.00384 12600.00 10000.00 0.00 -0.00353 12800.00 10000.00 0.00 -0.00311 13000.00 10000.00 0.00 -0.00267 13200.00 10000.00 0.00 -0.00220 13400.00 10000.00 0.00 -0.00162 13600.00 10000.00 0.00 -0.00100 13800.00 10000.00 0.00 -0.00025 14000.00 10000.00 0.00 0.00049 14200.00 10000.00 0.00 0.00129 14400.00 10000.00 0.00 0.00223 14600.00 10000.00 0.00 0.00302 14800.00 10000.00 0.00 0.00374 15000.00 10000.00 0.00 0.00457 15200.00 10000.00 0.00 0.00543 15400.00 10000.00 0.00 0.00634 15600.00 10000.00 0.00 0.00734 15800.00 10000.00 0.00 0.00835 16000.00 10000.00 0.00 0.00949 16200.00 10000.00 0.00 0.01083 16400.00 10000.00 0.00 0.01210 16600.00 10000.00 0.00 0.01319 16800.00 10000.00 0.00 0.01426 17000.00 10000.00 0.00 0.01543 17200.00 10000.00 0.00 0.01661 17400.00 10000.00 0.00 0.01781 17600.00 10000.00 0.00 0.01906 17800.00 10000.00 0.00 0.02027 18000.00 10000.00 0.00 0.02152 18200.00 10000.00 0.00 0.02279 18400.00 10000.00 0.00 0.02405 18600.00 10000.00 0.00 0.02530 18800.00 10000.00 0.00 0.02678 19000.00 10000.00 0.00 0.02836 19200.00 10000.00 0.00 0.02993 19400.00 10000.00 0.00 0.03160 19600.00 10000.00 0.00 0.03318 19800.00 10000.00 0.00 0.03485 20000.00 10000.00 0.00 0.03667 20200.00 10000.00 0.00 0.03848 20400.00 10000.00 0.00 0.04026 20600.00 10000.00 0.00 0.04192 20800.00 10000.00 0.00 0.04350 21000.00 10000.00 0.00 0.04505 21200.00 10000.00 0.00 0.04623 21400.00 10000.00 0.00 0.04697 21600.00 10000.00 0.00 0.04753 21800.00 10000.00 0.00 0.04784 22000.00 10000.00 0.00 0.04795 21600.00 10200.00 0.00 0.04818 21800.00 10200.00 0.00 0.04846 22000.00 10200.00 0.00 0.04856 21600.00 10400.00 0.00 0.04892 21800.00 10400.00 0.00 0.04917 22000.00 10400.00 0.00 0.04926 21600.00 10600.00 0.00 0.04960 21800.00 10600.00 0.00 0.04983 22000.00 10600.00 0.00 0.04995 22200.00 10600.00 0.00 0.04999 21600.00 10800.00 0.00 0.05034 21800.00 10800.00 0.00 0.05057 22000.00 10800.00 0.00 0.05072 22200.00 10800.00 0.00 0.05078 21600.00 11000.00 0.00 0.05118 21800.00 11000.00 0.00 0.05139 22000.00 11000.00 0.00 0.05156 22200.00 11000.00 0.00 0.05164 21600.00 11200.00 0.00 0.05189 21800.00 11200.00 0.00 0.05215 22000.00 11200.00 0.00 0.05239 22200.00 11200.00 0.00 0.05251 21600.00 11400.00 0.00 0.05267 21800.00 11400.00 0.00 0.05302 22000.00 11400.00 0.00 0.05330 22200.00 11400.00 0.00 0.05342 21600.00 11600.00 0.00 0.05358 21800.00 11600.00 0.00 0.05396 22000.00 11600.00 0.00 0.05420 22200.00 11600.00 0.00 0.05424 21600.00 11800.00 0.00 0.05454 21800.00 11800.00 0.00 0.05486 22000.00 11800.00 0.00 0.05500 21600.00 12000.00 0.00 0.05533 21800.00 12000.00 0.00 0.05557 22000.00 12000.00 0.00 0.05567 21400.00 12200.00 0.00 0.05571 21600.00 12200.00 0.00 0.05614 21800.00 12200.00 0.00 0.05635 22000.00 12200.00 0.00 0.05647 22200.00 12200.00 0.00 0.05650 21400.00 12400.00 0.00 0.05653 21600.00 12400.00 0.00 0.05693 21800.00 12400.00 0.00 0.05716 22000.00 12400.00 0.00 0.05732 22200.00 12400.00 0.00 0.05737 21600.00 12600.00 0.00 0.05773 21800.00 12600.00 0.00 0.05801 22000.00 12600.00 0.00 0.05822 22200.00 12600.00 0.00 0.05829 21400.00 12800.00 0.00 0.05843 21600.00 12800.00 0.00 0.05887 21800.00 12800.00 0.00 0.05919 22000.00 12800.00 0.00 0.05940 22200.00 12800.00 0.00 0.05943 21400.00 13000.00 0.00 0.05949 21600.00 13000.00 0.00 0.05993 21800.00 13000.00 0.00 0.06022 22000.00 13000.00 0.00 0.06038 21400.00 13200.00 0.00 0.06050 21600.00 13200.00 0.00 0.06093 21800.00 13200.00 0.00 0.06121 22000.00 13200.00 0.00 0.06134 21400.00 13400.00 0.00 0.06163 21600.00 13400.00 0.00 0.06204 21800.00 13400.00 0.00 0.06229 22000.00 13400.00 0.00 0.06241 21400.00 13600.00 0.00 0.06282 21600.00 13600.00 0.00 0.06317 21800.00 13600.00 0.00 0.06342 22000.00 13600.00 0.00 0.06355 21400.00 13800.00 0.00 0.06389 21600.00 13800.00 0.00 0.06427 21800.00 13800.00 0.00 0.06458 22000.00 13800.00 0.00 0.06474 21400.00 14000.00 0.00 0.06501 21600.00 14000.00 0.00 0.06547 21800.00 14000.00 0.00 0.06582 22000.00 14000.00 0.00 0.06595 21400.00 14200.00 0.00 0.06627 21600.00 14200.00 0.00 0.06677 21800.00 14200.00 0.00 0.06708 22000.00 14200.00 0.00 0.06713 21400.00 14400.00 0.00 0.06739 21600.00 14400.00 0.00 0.06788 21800.00 14400.00 0.00 0.06814 22000.00 14400.00 0.00 0.06816 21400.00 14600.00 0.00 0.06862 21600.00 14600.00 0.00 0.06911 21800.00 14600.00 0.00 0.06936 22000.00 14600.00 0.00 0.06937 21400.00 14800.00 0.00 0.06991 21600.00 14800.00 0.00 0.07039 21800.00 14800.00 0.00 0.07061 21400.00 15000.00 0.00 0.07120 21600.00 15000.00 0.00 0.07169 21800.00 15000.00 0.00 0.07189 21200.00 15200.00 0.00 0.07208 21400.00 15200.00 0.00 0.07269 21600.00 15200.00 0.00 0.07318 21800.00 15200.00 0.00 0.07334 21200.00 15400.00 0.00 0.07381 21400.00 15400.00 0.00 0.07438 21600.00 15400.00 0.00 0.07483 21800.00 15400.00 0.00 0.07494 21200.00 15600.00 0.00 0.07550 21400.00 15600.00 0.00 0.07601 21600.00 15600.00 0.00 0.07643 21800.00 15600.00 0.00 0.07652 21200.00 15800.00 0.00 0.07701 21400.00 15800.00 0.00 0.07754 21600.00 15800.00 0.00 0.07798 21800.00 15800.00 0.00 0.07806 21200.00 16000.00 0.00 0.07861 21400.00 16000.00 0.00 0.07917 21600.00 16000.00 0.00 0.07958 21800.00 16000.00 0.00 0.07959 21200.00 16200.00 0.00 0.08015 21400.00 16200.00 0.00 0.08071 21600.00 16200.00 0.00 0.08104 21200.00 16400.00 0.00 0.08194 21400.00 16400.00 0.00 0.08245 21600.00 16400.00 0.00 0.08273 21200.00 16600.00 0.00 0.08387 21400.00 16600.00 0.00 0.08434 21600.00 16600.00 0.00 0.08460 21200.00 16800.00 0.00 0.08576 21400.00 16800.00 0.00 0.08621 21600.00 16800.00 0.00 0.08643 21200.00 17000.00 0.00 0.08758 21400.00 17000.00 0.00 0.08795 21600.00 17000.00 0.00 0.08808 21000.00 17200.00 0.00 0.08818 21200.00 17200.00 0.00 0.08934 21400.00 17200.00 0.00 0.08960 21600.00 17200.00 0.00 0.08965 21000.00 17400.00 0.00 0.09014 21200.00 17400.00 0.00 0.09118 21400.00 17400.00 0.00 0.09135 21600.00 17400.00 0.00 0.09142 21000.00 17600.00 0.00 0.09203 21200.00 17600.00 0.00 0.09300 21400.00 17600.00 0.00 0.09317 21600.00 17600.00 0.00 0.09332 21000.00 17800.00 0.00 0.09411 21200.00 17800.00 0.00 0.09505 21400.00 17800.00 0.00 0.09526 21600.00 17800.00 0.00 0.09541 21000.00 18000.00 0.00 0.09616 21200.00 18000.00 0.00 0.09708 21400.00 18000.00 0.00 0.09729 21600.00 18000.00 0.00 0.09737 21000.00 18200.00 0.00 0.09835 21200.00 18200.00 0.00 0.09927 21400.00 18200.00 0.00 0.09943 21000.00 18400.00 0.00 0.10043 21200.00 18400.00 0.00 0.10132 21400.00 18400.00 0.00 0.10141 21000.00 18600.00 0.00 0.10241 21200.00 18600.00 0.00 0.10326 21400.00 18600.00 0.00 0.10328 21000.00 18800.00 0.00 0.10462 21200.00 18800.00 0.00 0.10543 21000.00 19000.00 0.00 0.10666 21200.00 19000.00 0.00 0.10739 21000.00 19200.00 0.00 0.10890 21200.00 19200.00 0.00 0.10954 21000.00 19400.00 0.00 0.11096 21200.00 19400.00 0.00 0.11151 20800.00 19600.00 0.00 0.11156 21000.00 19600.00 0.00 0.11301 21200.00 19600.00 0.00 0.11352 20800.00 19800.00 0.00 0.11388 21000.00 19800.00 0.00 0.11531 21200.00 19800.00 0.00 0.11579 20800.00 20000.00 0.00 0.11630 21000.00 20000.00 0.00 0.11765 21200.00 20000.00 0.00 0.11796 20800.00 20200.00 0.00 0.11858 21000.00 20200.00 0.00 0.11976 21200.00 20200.00 0.00 0.11983 20800.00 20400.00 0.00 0.12061 21000.00 20400.00 0.00 0.12156 20800.00 20600.00 0.00 0.12258 21000.00 20600.00 0.00 0.12320 21000.00 20800.00 0.00 0.12356 20800.00 21000.00 25.00 0.12364 20600.00 21000.00 30.00 0.12365 20800.00 21000.00 30.00 0.12375 20600.00 21000.00 35.00 0.12385 20600.00 21000.00 40.00 0.12401 20600.00 21000.00 45.00 0.12413 20600.00 21000.00 50.00 0.12421 20600.00 21000.00 55.00 0.12424 20600.00 21200.00 55.00 0.12424 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20643.10 21100.13 56.39 0.12452 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/161.ctf NX, NY, NZ: 512 512 1 MODE : real