CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/160.mrc Output diagnostic file name Micrographs_0601/160.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/160.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2456.000 7656.000 Mean, RMS : 4539.003 225.9031 TITLE 1: emi2mrc: 160.emi Sun Jan 05 16:52:22 2014 TITLE 2: kV 300, Mag 78000x, Def 23790, Spot 4, Int 55.10%, Obj 88.02%, Dif 73.67% TITLE 3: X 15.988, Y 320.031, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00972 10200.00 10000.00 0.00 -0.00967 10400.00 10000.00 0.00 -0.00961 15400.00 10000.00 0.00 -0.00926 15600.00 10000.00 0.00 -0.00883 15800.00 10000.00 0.00 -0.00830 16000.00 10000.00 0.00 -0.00768 16200.00 10000.00 0.00 -0.00692 16400.00 10000.00 0.00 -0.00615 16600.00 10000.00 0.00 -0.00528 16800.00 10000.00 0.00 -0.00432 17000.00 10000.00 0.00 -0.00349 17200.00 10000.00 0.00 -0.00258 17400.00 10000.00 0.00 -0.00173 17600.00 10000.00 0.00 -0.00099 17800.00 10000.00 0.00 -0.00015 18000.00 10000.00 0.00 0.00069 18200.00 10000.00 0.00 0.00153 18400.00 10000.00 0.00 0.00242 18600.00 10000.00 0.00 0.00342 18800.00 10000.00 0.00 0.00439 19000.00 10000.00 0.00 0.00532 19200.00 10000.00 0.00 0.00640 19400.00 10000.00 0.00 0.00739 19600.00 10000.00 0.00 0.00832 19800.00 10000.00 0.00 0.00932 20000.00 10000.00 0.00 0.01038 20200.00 10000.00 0.00 0.01152 20400.00 10000.00 0.00 0.01263 20600.00 10000.00 0.00 0.01379 20800.00 10000.00 0.00 0.01503 21000.00 10000.00 0.00 0.01623 21200.00 10000.00 0.00 0.01744 21400.00 10000.00 0.00 0.01874 21600.00 10000.00 0.00 0.02008 21800.00 10000.00 0.00 0.02143 22000.00 10000.00 0.00 0.02288 22200.00 10000.00 0.00 0.02447 22400.00 10000.00 0.00 0.02623 22600.00 10000.00 0.00 0.02813 22800.00 10000.00 0.00 0.02994 23000.00 10000.00 0.00 0.03167 23200.00 10000.00 0.00 0.03342 23400.00 10000.00 0.00 0.03514 23600.00 10000.00 0.00 0.03668 23800.00 10000.00 0.00 0.03780 24000.00 10000.00 0.00 0.03847 24200.00 10000.00 0.00 0.03880 24400.00 10000.00 0.00 0.03889 24200.00 10200.00 0.00 0.03917 24400.00 10200.00 0.00 0.03928 24200.00 10400.00 0.00 0.03964 24400.00 10400.00 0.00 0.03974 24200.00 10600.00 0.00 0.04002 24400.00 10600.00 0.00 0.04009 24600.00 10600.00 0.00 0.04010 24000.00 10800.00 0.00 0.04014 24200.00 10800.00 0.00 0.04048 24400.00 10800.00 0.00 0.04054 24600.00 10800.00 0.00 0.04055 24000.00 11000.00 0.00 0.04073 24200.00 11000.00 0.00 0.04101 24400.00 11000.00 0.00 0.04105 24600.00 11000.00 0.00 0.04106 24000.00 11200.00 0.00 0.04115 24200.00 11200.00 0.00 0.04139 24400.00 11200.00 0.00 0.04144 24600.00 11200.00 0.00 0.04146 24000.00 11400.00 0.00 0.04154 24200.00 11400.00 0.00 0.04178 24400.00 11400.00 0.00 0.04184 24600.00 11400.00 0.00 0.04186 24000.00 11600.00 0.00 0.04188 24200.00 11600.00 0.00 0.04211 24400.00 11600.00 0.00 0.04218 24600.00 11600.00 0.00 0.04220 24000.00 11800.00 0.00 0.04230 24200.00 11800.00 0.00 0.04252 24400.00 11800.00 0.00 0.04261 24600.00 11800.00 0.00 0.04263 24000.00 12000.00 0.00 0.04273 24200.00 12000.00 0.00 0.04295 24400.00 12000.00 0.00 0.04306 24600.00 12000.00 0.00 0.04307 24000.00 12200.00 0.00 0.04313 24200.00 12200.00 0.00 0.04337 24400.00 12200.00 0.00 0.04349 24000.00 12400.00 0.00 0.04365 24200.00 12400.00 0.00 0.04391 24400.00 12400.00 0.00 0.04405 24000.00 12600.00 0.00 0.04417 24200.00 12600.00 0.00 0.04446 24400.00 12600.00 0.00 0.04460 24000.00 12800.00 0.00 0.04469 24200.00 12800.00 0.00 0.04501 24400.00 12800.00 0.00 0.04514 24000.00 13000.00 0.00 0.04525 24200.00 13000.00 0.00 0.04559 24400.00 13000.00 0.00 0.04570 24000.00 13200.00 0.00 0.04594 24200.00 13200.00 0.00 0.04626 24400.00 13200.00 0.00 0.04634 24000.00 13400.00 0.00 0.04660 24200.00 13400.00 0.00 0.04692 24400.00 13400.00 0.00 0.04700 24000.00 13600.00 0.00 0.04743 24200.00 13600.00 0.00 0.04775 24400.00 13600.00 0.00 0.04782 24000.00 13800.00 0.00 0.04847 24200.00 13800.00 0.00 0.04874 24400.00 13800.00 0.00 0.04874 24000.00 14000.00 0.00 0.04940 24200.00 14000.00 0.00 0.04958 23800.00 14200.00 0.00 0.04968 24000.00 14200.00 0.00 0.05028 24200.00 14200.00 0.00 0.05040 23800.00 14400.00 0.00 0.05056 24000.00 14400.00 0.00 0.05110 24200.00 14400.00 0.00 0.05122 24400.00 14400.00 0.00 0.05123 23800.00 14600.00 0.00 0.05145 24000.00 14600.00 0.00 0.05197 24200.00 14600.00 0.00 0.05212 24400.00 14600.00 0.00 0.05216 23800.00 14800.00 0.00 0.05227 24000.00 14800.00 0.00 0.05279 24200.00 14800.00 0.00 0.05295 24400.00 14800.00 0.00 0.05300 23800.00 15000.00 0.00 0.05308 24000.00 15000.00 0.00 0.05358 24200.00 15000.00 0.00 0.05377 24400.00 15000.00 0.00 0.05385 23800.00 15200.00 0.00 0.05398 24000.00 15200.00 0.00 0.05451 24200.00 15200.00 0.00 0.05474 24400.00 15200.00 0.00 0.05486 23800.00 15400.00 0.00 0.05501 24000.00 15400.00 0.00 0.05557 24200.00 15400.00 0.00 0.05584 24400.00 15400.00 0.00 0.05597 23800.00 15600.00 0.00 0.05617 24000.00 15600.00 0.00 0.05674 24200.00 15600.00 0.00 0.05701 24400.00 15600.00 0.00 0.05712 23800.00 15800.00 0.00 0.05730 24000.00 15800.00 0.00 0.05786 24200.00 15800.00 0.00 0.05813 24400.00 15800.00 0.00 0.05822 23800.00 16000.00 0.00 0.05847 24000.00 16000.00 0.00 0.05906 24200.00 16000.00 0.00 0.05936 24400.00 16000.00 0.00 0.05945 23800.00 16200.00 0.00 0.05979 24000.00 16200.00 0.00 0.06042 24200.00 16200.00 0.00 0.06072 24400.00 16200.00 0.00 0.06078 23800.00 16400.00 0.00 0.06121 24000.00 16400.00 0.00 0.06184 24200.00 16400.00 0.00 0.06210 23800.00 16600.00 0.00 0.06252 24000.00 16600.00 0.00 0.06314 24200.00 16600.00 0.00 0.06336 23800.00 16800.00 0.00 0.06387 24000.00 16800.00 0.00 0.06448 24200.00 16800.00 0.00 0.06467 23800.00 17000.00 0.00 0.06530 24000.00 17000.00 0.00 0.06589 24200.00 17000.00 0.00 0.06604 23800.00 17200.00 0.00 0.06663 24000.00 17200.00 0.00 0.06720 24200.00 17200.00 0.00 0.06732 23800.00 17400.00 0.00 0.06804 24000.00 17400.00 0.00 0.06858 24200.00 17400.00 0.00 0.06869 23800.00 17600.00 0.00 0.06953 24000.00 17600.00 0.00 0.07002 24200.00 17600.00 0.00 0.07012 23800.00 17800.00 0.00 0.07102 24000.00 17800.00 0.00 0.07147 24200.00 17800.00 0.00 0.07156 23800.00 18000.00 0.00 0.07239 24000.00 18000.00 0.00 0.07283 24200.00 18000.00 0.00 0.07294 23800.00 18200.00 0.00 0.07388 24000.00 18200.00 0.00 0.07436 24200.00 18200.00 0.00 0.07446 23800.00 18400.00 0.00 0.07550 24000.00 18400.00 0.00 0.07598 24200.00 18400.00 0.00 0.07605 23800.00 18600.00 0.00 0.07711 24000.00 18600.00 0.00 0.07755 24200.00 18600.00 0.00 0.07756 23600.00 18800.00 0.00 0.07770 23800.00 18800.00 0.00 0.07874 24000.00 18800.00 0.00 0.07912 23600.00 19000.00 0.00 0.07942 23800.00 19000.00 0.00 0.08039 24000.00 19000.00 0.00 0.08076 24200.00 19000.00 0.00 0.08076 23600.00 19200.00 0.00 0.08124 23800.00 19200.00 0.00 0.08218 24000.00 19200.00 0.00 0.08259 24200.00 19200.00 0.00 0.08262 23600.00 19400.00 0.00 0.08299 23800.00 19400.00 0.00 0.08396 24000.00 19400.00 0.00 0.08441 23600.00 19600.00 0.00 0.08493 23800.00 19600.00 0.00 0.08595 24000.00 19600.00 0.00 0.08638 23600.00 19800.00 0.00 0.08692 23800.00 19800.00 0.00 0.08796 24000.00 19800.00 0.00 0.08838 23600.00 20000.00 0.00 0.08900 23800.00 20000.00 0.00 0.09008 24000.00 20000.00 0.00 0.09045 23600.00 20200.00 0.00 0.09121 23800.00 20200.00 0.00 0.09223 24000.00 20200.00 0.00 0.09252 23600.00 20400.00 0.00 0.09320 23800.00 20400.00 0.00 0.09416 24000.00 20400.00 0.00 0.09438 23600.00 20600.00 0.00 0.09541 23800.00 20600.00 0.00 0.09631 24000.00 20600.00 0.00 0.09647 23600.00 20800.00 0.00 0.09747 23800.00 20800.00 0.00 0.09830 24000.00 20800.00 0.00 0.09837 23600.00 21000.00 0.00 0.09943 23800.00 21000.00 0.00 0.10019 24000.00 21000.00 0.00 0.10024 23600.00 21200.00 0.00 0.10163 23800.00 21200.00 0.00 0.10239 24000.00 21200.00 0.00 0.10243 23600.00 21400.00 0.00 0.10413 23800.00 21400.00 0.00 0.10483 23400.00 21600.00 0.00 0.10495 23600.00 21600.00 0.00 0.10660 23800.00 21600.00 0.00 0.10721 23400.00 21800.00 0.00 0.10749 23600.00 21800.00 0.00 0.10909 23800.00 21800.00 0.00 0.10960 23400.00 22000.00 0.00 0.11019 23600.00 22000.00 0.00 0.11169 23800.00 22000.00 0.00 0.11206 23400.00 22200.00 0.00 0.11273 23600.00 22200.00 0.00 0.11413 23800.00 22200.00 0.00 0.11437 23400.00 22400.00 0.00 0.11549 23600.00 22400.00 0.00 0.11680 23800.00 22400.00 0.00 0.11691 23400.00 22600.00 0.00 0.11816 23600.00 22600.00 0.00 0.11938 23400.00 22800.00 0.00 0.12095 23600.00 22800.00 0.00 0.12200 23400.00 23000.00 0.00 0.12362 23600.00 23000.00 0.00 0.12437 23400.00 23200.00 0.00 0.12589 23600.00 23200.00 0.00 0.12619 23400.00 23400.00 0.00 0.12734 23400.00 23600.00 35.00 0.12736 23400.00 23600.00 40.00 0.12740 23400.00 23600.00 45.00 0.12741 23400.00 23600.00 50.00 0.12741 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23368.40 23509.77 50.00 0.12766 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/160.ctf NX, NY, NZ: 512 512 1 MODE : real