CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/154.mrc Output diagnostic file name Micrographs_0601/154.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/154.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2384.000 7916.000 Mean, RMS : 4527.110 221.8441 TITLE 1: emi2mrc: 154.emi Sun Jan 05 16:45:39 2014 TITLE 2: kV 300, Mag 78000x, Def 21990, Spot 4, Int 55.10%, Obj 88.03%, Dif 73.67% TITLE 3: X 16.879, Y 322.112, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00862 10200.00 10000.00 0.00 -0.00854 11400.00 10000.00 0.00 -0.00837 11600.00 10000.00 0.00 -0.00819 11800.00 10000.00 0.00 -0.00805 12000.00 10000.00 0.00 -0.00785 12200.00 10000.00 0.00 -0.00760 12400.00 10000.00 0.00 -0.00740 12600.00 10000.00 0.00 -0.00722 12800.00 10000.00 0.00 -0.00693 13000.00 10000.00 0.00 -0.00663 13200.00 10000.00 0.00 -0.00633 13400.00 10000.00 0.00 -0.00599 13600.00 10000.00 0.00 -0.00563 13800.00 10000.00 0.00 -0.00518 14000.00 10000.00 0.00 -0.00459 14200.00 10000.00 0.00 -0.00393 14400.00 10000.00 0.00 -0.00322 14600.00 10000.00 0.00 -0.00245 14800.00 10000.00 0.00 -0.00171 15000.00 10000.00 0.00 -0.00100 15200.00 10000.00 0.00 -0.00026 15400.00 10000.00 0.00 0.00054 15600.00 10000.00 0.00 0.00131 15800.00 10000.00 0.00 0.00204 16000.00 10000.00 0.00 0.00299 16200.00 10000.00 0.00 0.00405 16400.00 10000.00 0.00 0.00504 16600.00 10000.00 0.00 0.00606 16800.00 10000.00 0.00 0.00714 17000.00 10000.00 0.00 0.00840 17200.00 10000.00 0.00 0.00958 17400.00 10000.00 0.00 0.01058 17600.00 10000.00 0.00 0.01167 17800.00 10000.00 0.00 0.01282 18000.00 10000.00 0.00 0.01398 18200.00 10000.00 0.00 0.01523 18400.00 10000.00 0.00 0.01656 18600.00 10000.00 0.00 0.01776 18800.00 10000.00 0.00 0.01892 19000.00 10000.00 0.00 0.02023 19200.00 10000.00 0.00 0.02164 19400.00 10000.00 0.00 0.02320 19600.00 10000.00 0.00 0.02475 19800.00 10000.00 0.00 0.02616 20000.00 10000.00 0.00 0.02763 20200.00 10000.00 0.00 0.02926 20400.00 10000.00 0.00 0.03087 20600.00 10000.00 0.00 0.03235 20800.00 10000.00 0.00 0.03379 21000.00 10000.00 0.00 0.03533 21200.00 10000.00 0.00 0.03704 21400.00 10000.00 0.00 0.03893 21600.00 10000.00 0.00 0.04096 21800.00 10000.00 0.00 0.04290 22000.00 10000.00 0.00 0.04464 22200.00 10000.00 0.00 0.04581 22400.00 10000.00 0.00 0.04629 22600.00 10000.00 0.00 0.04666 22800.00 10000.00 0.00 0.04690 22600.00 10200.00 0.00 0.04710 22800.00 10200.00 0.00 0.04730 22600.00 10400.00 0.00 0.04751 22800.00 10400.00 0.00 0.04765 22600.00 10600.00 0.00 0.04790 22800.00 10600.00 0.00 0.04800 22600.00 10800.00 0.00 0.04832 22800.00 10800.00 0.00 0.04841 22400.00 11000.00 0.00 0.04863 22600.00 11000.00 0.00 0.04902 22800.00 11000.00 0.00 0.04908 22400.00 11200.00 0.00 0.04931 22600.00 11200.00 0.00 0.04965 22400.00 11400.00 0.00 0.04995 22600.00 11400.00 0.00 0.05023 22400.00 11600.00 0.00 0.05065 22600.00 11600.00 0.00 0.05093 22800.00 11600.00 0.00 0.05094 22400.00 11800.00 0.00 0.05134 22600.00 11800.00 0.00 0.05162 22800.00 11800.00 0.00 0.05163 22400.00 12000.00 0.00 0.05202 22600.00 12000.00 0.00 0.05227 22400.00 12200.00 0.00 0.05274 22600.00 12200.00 0.00 0.05298 22200.00 12400.00 0.00 0.05310 22400.00 12400.00 0.00 0.05361 22600.00 12400.00 0.00 0.05384 22200.00 12600.00 0.00 0.05400 22400.00 12600.00 0.00 0.05450 22600.00 12600.00 0.00 0.05471 22200.00 12800.00 0.00 0.05484 22400.00 12800.00 0.00 0.05532 22600.00 12800.00 0.00 0.05550 22800.00 12800.00 0.00 0.05551 22200.00 13000.00 0.00 0.05568 22400.00 13000.00 0.00 0.05613 22600.00 13000.00 0.00 0.05633 22800.00 13000.00 0.00 0.05639 22200.00 13200.00 0.00 0.05661 22400.00 13200.00 0.00 0.05706 22600.00 13200.00 0.00 0.05729 22800.00 13200.00 0.00 0.05740 22200.00 13400.00 0.00 0.05751 22400.00 13400.00 0.00 0.05797 22600.00 13400.00 0.00 0.05824 22800.00 13400.00 0.00 0.05836 22200.00 13600.00 0.00 0.05849 22400.00 13600.00 0.00 0.05897 22600.00 13600.00 0.00 0.05926 22800.00 13600.00 0.00 0.05938 22200.00 13800.00 0.00 0.05954 22400.00 13800.00 0.00 0.06004 22600.00 13800.00 0.00 0.06034 22800.00 13800.00 0.00 0.06042 22200.00 14000.00 0.00 0.06062 22400.00 14000.00 0.00 0.06111 22600.00 14000.00 0.00 0.06140 22800.00 14000.00 0.00 0.06144 22200.00 14200.00 0.00 0.06180 22400.00 14200.00 0.00 0.06227 22600.00 14200.00 0.00 0.06253 22800.00 14200.00 0.00 0.06255 22200.00 14400.00 0.00 0.06298 22400.00 14400.00 0.00 0.06344 22600.00 14400.00 0.00 0.06370 22800.00 14400.00 0.00 0.06372 22200.00 14600.00 0.00 0.06428 22400.00 14600.00 0.00 0.06476 22600.00 14600.00 0.00 0.06505 22800.00 14600.00 0.00 0.06506 22200.00 14800.00 0.00 0.06565 22400.00 14800.00 0.00 0.06615 22600.00 14800.00 0.00 0.06642 22200.00 15000.00 0.00 0.06713 22400.00 15000.00 0.00 0.06759 22600.00 15000.00 0.00 0.06783 22200.00 15200.00 0.00 0.06849 22400.00 15200.00 0.00 0.06892 22600.00 15200.00 0.00 0.06913 22200.00 15400.00 0.00 0.06967 22400.00 15400.00 0.00 0.07009 22600.00 15400.00 0.00 0.07030 22200.00 15600.00 0.00 0.07089 22400.00 15600.00 0.00 0.07132 22600.00 15600.00 0.00 0.07154 22200.00 15800.00 0.00 0.07216 22400.00 15800.00 0.00 0.07262 22600.00 15800.00 0.00 0.07285 22200.00 16000.00 0.00 0.07358 22400.00 16000.00 0.00 0.07407 22600.00 16000.00 0.00 0.07426 22200.00 16200.00 0.00 0.07501 22400.00 16200.00 0.00 0.07548 22600.00 16200.00 0.00 0.07557 22200.00 16400.00 0.00 0.07654 22400.00 16400.00 0.00 0.07693 22200.00 16600.00 0.00 0.07799 22400.00 16600.00 0.00 0.07830 22200.00 16800.00 0.00 0.07944 22400.00 16800.00 0.00 0.07968 22000.00 17000.00 0.00 0.07976 22200.00 17000.00 0.00 0.08096 22400.00 17000.00 0.00 0.08119 22000.00 17200.00 0.00 0.08130 22200.00 17200.00 0.00 0.08253 22400.00 17200.00 0.00 0.08276 22000.00 17400.00 0.00 0.08303 22200.00 17400.00 0.00 0.08429 22400.00 17400.00 0.00 0.08449 22000.00 17600.00 0.00 0.08477 22200.00 17600.00 0.00 0.08601 22400.00 17600.00 0.00 0.08615 22000.00 17800.00 0.00 0.08658 22200.00 17800.00 0.00 0.08777 22400.00 17800.00 0.00 0.08786 22000.00 18000.00 0.00 0.08840 22200.00 18000.00 0.00 0.08955 22400.00 18000.00 0.00 0.08959 22000.00 18200.00 0.00 0.09021 22200.00 18200.00 0.00 0.09131 22000.00 18400.00 0.00 0.09194 22200.00 18400.00 0.00 0.09300 22000.00 18600.00 0.00 0.09384 22200.00 18600.00 0.00 0.09486 22000.00 18800.00 0.00 0.09585 22200.00 18800.00 0.00 0.09685 22000.00 19000.00 0.00 0.09783 22200.00 19000.00 0.00 0.09880 22000.00 19200.00 0.00 0.10005 22200.00 19200.00 0.00 0.10099 22000.00 19400.00 0.00 0.10219 22200.00 19400.00 0.00 0.10309 22000.00 19600.00 0.00 0.10450 22200.00 19600.00 0.00 0.10536 22000.00 19800.00 0.00 0.10686 22200.00 19800.00 0.00 0.10765 22000.00 20000.00 0.00 0.10923 22200.00 20000.00 0.00 0.10994 22000.00 20200.00 0.00 0.11167 22200.00 20200.00 0.00 0.11226 22000.00 20400.00 0.00 0.11410 22200.00 20400.00 0.00 0.11456 21800.00 20600.00 0.00 0.11463 22000.00 20600.00 0.00 0.11677 22200.00 20600.00 0.00 0.11702 21800.00 20800.00 0.00 0.11713 22000.00 20800.00 0.00 0.11916 21800.00 21000.00 0.00 0.11982 22000.00 21000.00 0.00 0.12170 21800.00 21200.00 0.00 0.12253 22000.00 21200.00 0.00 0.12421 21800.00 21400.00 0.00 0.12518 22000.00 21400.00 0.00 0.12654 21800.00 21600.00 0.00 0.12741 22000.00 21600.00 0.00 0.12836 21800.00 21800.00 0.00 0.12885 22000.00 21800.00 0.00 0.12938 21800.00 22000.00 0.00 0.12953 21800.00 22000.00 5.00 0.12965 21800.00 22000.00 10.00 0.12976 21800.00 22000.00 15.00 0.12986 21800.00 22000.00 20.00 0.12996 21800.00 22000.00 25.00 0.13004 21800.00 22000.00 30.00 0.13011 21800.00 22000.00 35.00 0.13016 21800.00 22000.00 40.00 0.13019 21800.00 22000.00 45.00 0.13020 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21833.98 22046.42 42.60 0.13034 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/154.ctf NX, NY, NZ: 512 512 1 MODE : real