CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/151.mrc Output diagnostic file name Micrographs_0601/151.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/151.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2162.000 7996.000 Mean, RMS : 4531.429 224.5838 TITLE 1: emi2mrc: 151.emi Sun Jan 05 16:42:47 2014 TITLE 2: kV 300, Mag 78000x, Def 21990, Spot 4, Int 55.10%, Obj 88.03%, Dif 73.67% TITLE 3: X 14.084, Y 325.483, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01591 14000.00 10000.00 0.00 -0.01589 14200.00 10000.00 0.00 -0.01567 14400.00 10000.00 0.00 -0.01543 14600.00 10000.00 0.00 -0.01513 14800.00 10000.00 0.00 -0.01477 15000.00 10000.00 0.00 -0.01433 15200.00 10000.00 0.00 -0.01399 15400.00 10000.00 0.00 -0.01366 15600.00 10000.00 0.00 -0.01342 15800.00 10000.00 0.00 -0.01326 16000.00 10000.00 0.00 -0.01302 16200.00 10000.00 0.00 -0.01281 16400.00 10000.00 0.00 -0.01253 16600.00 10000.00 0.00 -0.01221 16800.00 10000.00 0.00 -0.01200 17000.00 10000.00 0.00 -0.01175 17200.00 10000.00 0.00 -0.01133 17400.00 10000.00 0.00 -0.01080 17600.00 10000.00 0.00 -0.01030 17800.00 10000.00 0.00 -0.00985 18000.00 10000.00 0.00 -0.00940 18200.00 10000.00 0.00 -0.00882 18400.00 10000.00 0.00 -0.00815 18600.00 10000.00 0.00 -0.00749 18800.00 10000.00 0.00 -0.00689 19000.00 10000.00 0.00 -0.00636 19200.00 10000.00 0.00 -0.00574 19400.00 10000.00 0.00 -0.00499 19600.00 10000.00 0.00 -0.00429 19800.00 10000.00 0.00 -0.00363 20000.00 10000.00 0.00 -0.00284 20200.00 10000.00 0.00 -0.00199 20400.00 10000.00 0.00 -0.00117 20600.00 10000.00 0.00 -0.00030 20800.00 10000.00 0.00 0.00062 21000.00 10000.00 0.00 0.00150 21200.00 10000.00 0.00 0.00226 21400.00 10000.00 0.00 0.00310 21600.00 10000.00 0.00 0.00400 21800.00 10000.00 0.00 0.00487 22000.00 10000.00 0.00 0.00571 22200.00 10000.00 0.00 0.00667 22400.00 10000.00 0.00 0.00785 22600.00 10000.00 0.00 0.00906 22800.00 10000.00 0.00 0.01025 23000.00 10000.00 0.00 0.01134 23200.00 10000.00 0.00 0.01240 23400.00 10000.00 0.00 0.01351 23600.00 10000.00 0.00 0.01444 23800.00 10000.00 0.00 0.01550 24000.00 10000.00 0.00 0.01678 24200.00 10000.00 0.00 0.01817 24400.00 10000.00 0.00 0.01963 24600.00 10000.00 0.00 0.02113 24800.00 10000.00 0.00 0.02280 25000.00 10000.00 0.00 0.02453 25200.00 10000.00 0.00 0.02629 25400.00 10000.00 0.00 0.02783 25600.00 10000.00 0.00 0.02899 25800.00 10000.00 0.00 0.02981 26000.00 10000.00 0.00 0.03027 26200.00 10000.00 0.00 0.03064 26400.00 10000.00 0.00 0.03089 26200.00 10200.00 0.00 0.03110 26400.00 10200.00 0.00 0.03133 26200.00 10400.00 0.00 0.03144 26400.00 10400.00 0.00 0.03166 26200.00 10600.00 0.00 0.03173 26400.00 10600.00 0.00 0.03196 26200.00 10800.00 0.00 0.03217 26400.00 10800.00 0.00 0.03237 26200.00 11000.00 0.00 0.03267 26400.00 11000.00 0.00 0.03280 26200.00 11200.00 0.00 0.03310 26400.00 11200.00 0.00 0.03315 26200.00 11400.00 0.00 0.03340 26400.00 11400.00 0.00 0.03342 26200.00 11600.00 0.00 0.03373 26400.00 11600.00 0.00 0.03376 26000.00 11800.00 0.00 0.03382 26200.00 11800.00 0.00 0.03415 26400.00 11800.00 0.00 0.03415 26200.00 12000.00 0.00 0.03438 26400.00 12000.00 0.00 0.03439 26200.00 12200.00 0.00 0.03466 26400.00 12200.00 0.00 0.03471 26200.00 12400.00 0.00 0.03502 26400.00 12400.00 0.00 0.03508 26200.00 12600.00 0.00 0.03542 26400.00 12600.00 0.00 0.03544 26000.00 12800.00 0.00 0.03563 26200.00 12800.00 0.00 0.03598 26000.00 13000.00 0.00 0.03619 26200.00 13000.00 0.00 0.03647 26000.00 13200.00 0.00 0.03673 26200.00 13200.00 0.00 0.03696 25800.00 13400.00 0.00 0.03702 26000.00 13400.00 0.00 0.03732 26200.00 13400.00 0.00 0.03757 26000.00 13600.00 0.00 0.03785 26200.00 13600.00 0.00 0.03813 26000.00 13800.00 0.00 0.03838 26200.00 13800.00 0.00 0.03868 26000.00 14000.00 0.00 0.03891 26200.00 14000.00 0.00 0.03918 26000.00 14200.00 0.00 0.03942 26200.00 14200.00 0.00 0.03967 25800.00 14400.00 0.00 0.03970 26000.00 14400.00 0.00 0.04003 26200.00 14400.00 0.00 0.04025 26000.00 14600.00 0.00 0.04055 26200.00 14600.00 0.00 0.04080 26000.00 14800.00 0.00 0.04104 26200.00 14800.00 0.00 0.04131 25800.00 15000.00 0.00 0.04139 26000.00 15000.00 0.00 0.04175 26200.00 15000.00 0.00 0.04204 25800.00 15200.00 0.00 0.04207 26000.00 15200.00 0.00 0.04243 26200.00 15200.00 0.00 0.04271 25800.00 15400.00 0.00 0.04273 26000.00 15400.00 0.00 0.04312 26200.00 15400.00 0.00 0.04344 25800.00 15600.00 0.00 0.04354 26000.00 15600.00 0.00 0.04399 26200.00 15600.00 0.00 0.04434 25800.00 15800.00 0.00 0.04444 26000.00 15800.00 0.00 0.04494 26200.00 15800.00 0.00 0.04524 25800.00 16000.00 0.00 0.04533 26000.00 16000.00 0.00 0.04581 26200.00 16000.00 0.00 0.04604 25800.00 16200.00 0.00 0.04608 26000.00 16200.00 0.00 0.04654 26200.00 16200.00 0.00 0.04673 25800.00 16400.00 0.00 0.04700 26000.00 16400.00 0.00 0.04743 26200.00 16400.00 0.00 0.04761 25800.00 16600.00 0.00 0.04791 26000.00 16600.00 0.00 0.04831 26200.00 16600.00 0.00 0.04843 25800.00 16800.00 0.00 0.04883 26000.00 16800.00 0.00 0.04917 26200.00 16800.00 0.00 0.04924 25800.00 17000.00 0.00 0.04977 26000.00 17000.00 0.00 0.05006 26200.00 17000.00 0.00 0.05011 25800.00 17200.00 0.00 0.05057 26000.00 17200.00 0.00 0.05084 26200.00 17200.00 0.00 0.05090 25800.00 17400.00 0.00 0.05140 26000.00 17400.00 0.00 0.05167 26200.00 17400.00 0.00 0.05172 25800.00 17600.00 0.00 0.05224 26000.00 17600.00 0.00 0.05251 26200.00 17600.00 0.00 0.05255 25600.00 17800.00 0.00 0.05259 25800.00 17800.00 0.00 0.05324 26000.00 17800.00 0.00 0.05350 26200.00 17800.00 0.00 0.05354 25600.00 18000.00 0.00 0.05360 25800.00 18000.00 0.00 0.05425 26000.00 18000.00 0.00 0.05452 26200.00 18000.00 0.00 0.05457 25600.00 18200.00 0.00 0.05467 25800.00 18200.00 0.00 0.05535 26000.00 18200.00 0.00 0.05562 26200.00 18200.00 0.00 0.05568 25600.00 18400.00 0.00 0.05589 25800.00 18400.00 0.00 0.05659 26000.00 18400.00 0.00 0.05686 26200.00 18400.00 0.00 0.05691 25600.00 18600.00 0.00 0.05715 25800.00 18600.00 0.00 0.05785 26000.00 18600.00 0.00 0.05808 26200.00 18600.00 0.00 0.05810 25600.00 18800.00 0.00 0.05846 25800.00 18800.00 0.00 0.05913 26000.00 18800.00 0.00 0.05932 25600.00 19000.00 0.00 0.05964 25800.00 19000.00 0.00 0.06028 26000.00 19000.00 0.00 0.06045 26200.00 19000.00 0.00 0.06046 25600.00 19200.00 0.00 0.06083 25800.00 19200.00 0.00 0.06146 26000.00 19200.00 0.00 0.06167 26200.00 19200.00 0.00 0.06175 25600.00 19400.00 0.00 0.06208 25800.00 19400.00 0.00 0.06274 26000.00 19400.00 0.00 0.06300 26200.00 19400.00 0.00 0.06313 25600.00 19600.00 0.00 0.06337 25800.00 19600.00 0.00 0.06408 26000.00 19600.00 0.00 0.06439 26200.00 19600.00 0.00 0.06452 25600.00 19800.00 0.00 0.06476 25800.00 19800.00 0.00 0.06552 26000.00 19800.00 0.00 0.06585 26200.00 19800.00 0.00 0.06594 25600.00 20000.00 0.00 0.06631 25800.00 20000.00 0.00 0.06709 26000.00 20000.00 0.00 0.06738 26200.00 20000.00 0.00 0.06739 25600.00 20200.00 0.00 0.06796 25800.00 20200.00 0.00 0.06870 26000.00 20200.00 0.00 0.06891 25600.00 20400.00 0.00 0.06947 25800.00 20400.00 0.00 0.07011 26000.00 20400.00 0.00 0.07026 25600.00 20600.00 0.00 0.07089 25800.00 20600.00 0.00 0.07148 26000.00 20600.00 0.00 0.07162 25600.00 20800.00 0.00 0.07225 25800.00 20800.00 0.00 0.07286 26000.00 20800.00 0.00 0.07304 25600.00 21000.00 0.00 0.07378 25800.00 21000.00 0.00 0.07441 26000.00 21000.00 0.00 0.07461 25600.00 21200.00 0.00 0.07548 25800.00 21200.00 0.00 0.07614 26000.00 21200.00 0.00 0.07632 25600.00 21400.00 0.00 0.07726 25800.00 21400.00 0.00 0.07790 26000.00 21400.00 0.00 0.07802 25600.00 21600.00 0.00 0.07902 25800.00 21600.00 0.00 0.07963 26000.00 21600.00 0.00 0.07969 25600.00 21800.00 0.00 0.08073 25800.00 21800.00 0.00 0.08130 26000.00 21800.00 0.00 0.08135 25600.00 22000.00 0.00 0.08242 25800.00 22000.00 0.00 0.08299 26000.00 22000.00 0.00 0.08302 25600.00 22200.00 0.00 0.08405 25800.00 22200.00 0.00 0.08462 25600.00 22400.00 0.00 0.08570 25800.00 22400.00 0.00 0.08623 25600.00 22600.00 0.00 0.08728 25800.00 22600.00 0.00 0.08776 25600.00 22800.00 0.00 0.08896 25800.00 22800.00 0.00 0.08936 25400.00 23000.00 0.00 0.08949 25600.00 23000.00 0.00 0.09079 25800.00 23000.00 0.00 0.09112 25400.00 23200.00 0.00 0.09149 25600.00 23200.00 0.00 0.09273 25800.00 23200.00 0.00 0.09300 25400.00 23400.00 0.00 0.09356 25600.00 23400.00 0.00 0.09477 25800.00 23400.00 0.00 0.09500 25400.00 23600.00 0.00 0.09572 25600.00 23600.00 0.00 0.09689 25800.00 23600.00 0.00 0.09704 25400.00 23800.00 0.00 0.09798 25600.00 23800.00 0.00 0.09908 25800.00 23800.00 0.00 0.09915 25400.00 24000.00 0.00 0.10023 25600.00 24000.00 0.00 0.10128 25400.00 24200.00 0.00 0.10263 25600.00 24200.00 0.00 0.10360 25400.00 24400.00 0.00 0.10496 25600.00 24400.00 0.00 0.10585 25400.00 24600.00 0.00 0.10761 25600.00 24600.00 0.00 0.10833 25200.00 24800.00 0.00 0.10841 25400.00 24800.00 0.00 0.11028 25600.00 24800.00 0.00 0.11078 25200.00 25000.00 0.00 0.11103 25400.00 25000.00 0.00 0.11273 25600.00 25000.00 0.00 0.11296 25200.00 25200.00 0.00 0.11379 25400.00 25200.00 0.00 0.11510 25200.00 25400.00 0.00 0.11554 25400.00 25400.00 0.00 0.11628 25200.00 25600.00 10.00 0.11633 25400.00 25600.00 10.00 0.11633 25200.00 25600.00 15.00 0.11653 25200.00 25600.00 20.00 0.11671 25200.00 25600.00 25.00 0.11685 25200.00 25600.00 30.00 0.11694 25200.00 25600.00 35.00 0.11697 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25259.36 25564.34 35.78 0.11707 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/151.ctf NX, NY, NZ: 512 512 1 MODE : real