CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/148.mrc Output diagnostic file name Micrographs_0601/148.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/148.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2530.000 9260.000 Mean, RMS : 4513.229 220.1358 TITLE 1: emi2mrc: 148.emi Sun Jan 05 16:39:06 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 24.582, Y 323.910, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02244 10200.00 10000.00 0.00 0.02356 10400.00 10000.00 0.00 0.02440 10600.00 10000.00 0.00 0.02553 10800.00 10000.00 0.00 0.02689 11000.00 10000.00 0.00 0.02825 11200.00 10000.00 0.00 0.02961 11400.00 10000.00 0.00 0.03092 11600.00 10000.00 0.00 0.03229 11800.00 10000.00 0.00 0.03385 12000.00 10000.00 0.00 0.03556 12200.00 10000.00 0.00 0.03730 12400.00 10000.00 0.00 0.03914 12600.00 10000.00 0.00 0.04104 12800.00 10000.00 0.00 0.04311 13000.00 10000.00 0.00 0.04512 13200.00 10000.00 0.00 0.04715 13400.00 10000.00 0.00 0.04939 13600.00 10000.00 0.00 0.05172 13800.00 10000.00 0.00 0.05418 14000.00 10000.00 0.00 0.05650 14200.00 10000.00 0.00 0.05888 14400.00 10000.00 0.00 0.06133 14600.00 10000.00 0.00 0.06384 14800.00 10000.00 0.00 0.06651 15000.00 10000.00 0.00 0.06922 15200.00 10000.00 0.00 0.07212 15400.00 10000.00 0.00 0.07498 15600.00 10000.00 0.00 0.07803 15800.00 10000.00 0.00 0.08104 16000.00 10000.00 0.00 0.08300 16200.00 10000.00 0.00 0.08377 16400.00 10000.00 0.00 0.08401 16600.00 10000.00 0.00 0.08406 16000.00 10200.00 0.00 0.08444 16200.00 10200.00 0.00 0.08519 16400.00 10200.00 0.00 0.08540 16600.00 10200.00 0.00 0.08545 16000.00 10400.00 0.00 0.08600 16200.00 10400.00 0.00 0.08675 16400.00 10400.00 0.00 0.08696 16600.00 10400.00 0.00 0.08704 16000.00 10600.00 0.00 0.08778 16200.00 10600.00 0.00 0.08852 16400.00 10600.00 0.00 0.08871 16600.00 10600.00 0.00 0.08879 16000.00 10800.00 0.00 0.08958 16200.00 10800.00 0.00 0.09027 16400.00 10800.00 0.00 0.09043 16600.00 10800.00 0.00 0.09046 16000.00 11000.00 0.00 0.09158 16200.00 11000.00 0.00 0.09222 16400.00 11000.00 0.00 0.09234 16600.00 11000.00 0.00 0.09237 16000.00 11200.00 0.00 0.09356 16200.00 11200.00 0.00 0.09419 16400.00 11200.00 0.00 0.09431 16600.00 11200.00 0.00 0.09432 16000.00 11400.00 0.00 0.09570 16200.00 11400.00 0.00 0.09632 16400.00 11400.00 0.00 0.09641 16000.00 11600.00 0.00 0.09790 16200.00 11600.00 0.00 0.09850 16400.00 11600.00 0.00 0.09853 16000.00 11800.00 0.00 0.10034 16200.00 11800.00 0.00 0.10085 16000.00 12000.00 0.00 0.10268 16200.00 12000.00 0.00 0.10310 16000.00 12200.00 0.00 0.10490 16200.00 12200.00 0.00 0.10529 15800.00 12400.00 0.00 0.10547 16000.00 12400.00 0.00 0.10731 16200.00 12400.00 0.00 0.10770 15800.00 12600.00 0.00 0.10789 16000.00 12600.00 0.00 0.10976 16200.00 12600.00 0.00 0.11016 15800.00 12800.00 0.00 0.11071 16000.00 12800.00 0.00 0.11260 16200.00 12800.00 0.00 0.11294 15800.00 13000.00 0.00 0.11346 16000.00 13000.00 0.00 0.11532 16200.00 13000.00 0.00 0.11554 15800.00 13200.00 0.00 0.11631 16000.00 13200.00 0.00 0.11811 16200.00 13200.00 0.00 0.11822 15800.00 13400.00 0.00 0.11922 16000.00 13400.00 0.00 0.12099 16200.00 13400.00 0.00 0.12102 15800.00 13600.00 0.00 0.12227 16000.00 13600.00 0.00 0.12397 15800.00 13800.00 0.00 0.12533 16000.00 13800.00 0.00 0.12696 15800.00 14000.00 0.00 0.12824 16000.00 14000.00 0.00 0.12982 15800.00 14200.00 0.00 0.13150 16000.00 14200.00 0.00 0.13301 15800.00 14400.00 0.00 0.13452 16000.00 14400.00 0.00 0.13591 15800.00 14600.00 0.00 0.13772 16000.00 14600.00 0.00 0.13897 15800.00 14800.00 0.00 0.14071 16000.00 14800.00 0.00 0.14184 15800.00 15000.00 0.00 0.14385 16000.00 15000.00 0.00 0.14477 15800.00 15200.00 0.00 0.14719 16000.00 15200.00 0.00 0.14780 15800.00 15400.00 0.00 0.15019 16000.00 15400.00 0.00 0.15038 15600.00 15600.00 0.00 0.15053 15800.00 15600.00 0.00 0.15331 15800.00 15800.00 0.00 0.15514 15800.00 16000.00 0.00 0.15529 15800.00 16000.00 5.00 0.15532 15800.00 16000.00 10.00 0.15532 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15745.49 15929.51 20.34 0.15566 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/148.ctf NX, NY, NZ: 512 512 1 MODE : real