CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/146.mrc Output diagnostic file name Micrographs_0601/146.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/146.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2148.000 8952.000 Mean, RMS : 4508.360 221.2657 TITLE 1: emi2mrc: 146.emi Sun Jan 05 16:36:35 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 20.071, Y 326.969, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00845 10200.00 10000.00 0.00 0.00868 10400.00 10000.00 0.00 0.00889 10600.00 10000.00 0.00 0.00940 10800.00 10000.00 0.00 0.00987 11000.00 10000.00 0.00 0.01029 11200.00 10000.00 0.00 0.01079 11400.00 10000.00 0.00 0.01133 11600.00 10000.00 0.00 0.01188 11800.00 10000.00 0.00 0.01250 12000.00 10000.00 0.00 0.01326 12200.00 10000.00 0.00 0.01413 12400.00 10000.00 0.00 0.01506 12600.00 10000.00 0.00 0.01604 12800.00 10000.00 0.00 0.01695 13000.00 10000.00 0.00 0.01778 13200.00 10000.00 0.00 0.01868 13400.00 10000.00 0.00 0.01956 13600.00 10000.00 0.00 0.02047 13800.00 10000.00 0.00 0.02150 14000.00 10000.00 0.00 0.02246 14200.00 10000.00 0.00 0.02348 14400.00 10000.00 0.00 0.02458 14600.00 10000.00 0.00 0.02575 14800.00 10000.00 0.00 0.02707 15000.00 10000.00 0.00 0.02841 15200.00 10000.00 0.00 0.02963 15400.00 10000.00 0.00 0.03085 15600.00 10000.00 0.00 0.03224 15800.00 10000.00 0.00 0.03366 16000.00 10000.00 0.00 0.03519 16200.00 10000.00 0.00 0.03668 16400.00 10000.00 0.00 0.03816 16600.00 10000.00 0.00 0.03976 16800.00 10000.00 0.00 0.04131 17000.00 10000.00 0.00 0.04291 17200.00 10000.00 0.00 0.04450 17400.00 10000.00 0.00 0.04620 17600.00 10000.00 0.00 0.04808 17800.00 10000.00 0.00 0.04997 18000.00 10000.00 0.00 0.05172 18200.00 10000.00 0.00 0.05352 18400.00 10000.00 0.00 0.05558 18600.00 10000.00 0.00 0.05759 18800.00 10000.00 0.00 0.05967 19000.00 10000.00 0.00 0.06173 19200.00 10000.00 0.00 0.06348 19400.00 10000.00 0.00 0.06466 19600.00 10000.00 0.00 0.06534 19800.00 10000.00 0.00 0.06588 20000.00 10000.00 0.00 0.06617 20200.00 10000.00 0.00 0.06628 20400.00 10000.00 0.00 0.06629 19800.00 10200.00 0.00 0.06667 20000.00 10200.00 0.00 0.06697 20200.00 10200.00 0.00 0.06709 20400.00 10200.00 0.00 0.06714 19800.00 10400.00 0.00 0.06768 20000.00 10400.00 0.00 0.06799 20200.00 10400.00 0.00 0.06813 20400.00 10400.00 0.00 0.06820 19600.00 10600.00 0.00 0.06827 19800.00 10600.00 0.00 0.06887 20000.00 10600.00 0.00 0.06915 20200.00 10600.00 0.00 0.06927 20400.00 10600.00 0.00 0.06930 19600.00 10800.00 0.00 0.06937 19800.00 10800.00 0.00 0.06993 20000.00 10800.00 0.00 0.07014 20200.00 10800.00 0.00 0.07024 19600.00 11000.00 0.00 0.07052 19800.00 11000.00 0.00 0.07098 20000.00 11000.00 0.00 0.07116 20200.00 11000.00 0.00 0.07125 19600.00 11200.00 0.00 0.07143 19800.00 11200.00 0.00 0.07186 20000.00 11200.00 0.00 0.07202 20200.00 11200.00 0.00 0.07211 19600.00 11400.00 0.00 0.07240 19800.00 11400.00 0.00 0.07284 20000.00 11400.00 0.00 0.07301 20200.00 11400.00 0.00 0.07309 19600.00 11600.00 0.00 0.07351 19800.00 11600.00 0.00 0.07392 20000.00 11600.00 0.00 0.07407 20200.00 11600.00 0.00 0.07413 19600.00 11800.00 0.00 0.07472 19800.00 11800.00 0.00 0.07510 20000.00 11800.00 0.00 0.07524 20200.00 11800.00 0.00 0.07532 19400.00 12000.00 0.00 0.07533 19600.00 12000.00 0.00 0.07600 19800.00 12000.00 0.00 0.07638 20000.00 12000.00 0.00 0.07656 20200.00 12000.00 0.00 0.07666 19400.00 12200.00 0.00 0.07670 19600.00 12200.00 0.00 0.07737 19800.00 12200.00 0.00 0.07775 20000.00 12200.00 0.00 0.07791 20200.00 12200.00 0.00 0.07795 19400.00 12400.00 0.00 0.07816 19600.00 12400.00 0.00 0.07877 19800.00 12400.00 0.00 0.07910 20000.00 12400.00 0.00 0.07921 19400.00 12600.00 0.00 0.07951 19600.00 12600.00 0.00 0.08006 19800.00 12600.00 0.00 0.08035 20000.00 12600.00 0.00 0.08043 19400.00 12800.00 0.00 0.08096 19600.00 12800.00 0.00 0.08147 19800.00 12800.00 0.00 0.08172 20000.00 12800.00 0.00 0.08178 19400.00 13000.00 0.00 0.08229 19600.00 13000.00 0.00 0.08275 19800.00 13000.00 0.00 0.08298 20000.00 13000.00 0.00 0.08303 19400.00 13200.00 0.00 0.08371 19600.00 13200.00 0.00 0.08415 19800.00 13200.00 0.00 0.08438 20000.00 13200.00 0.00 0.08441 19400.00 13400.00 0.00 0.08525 19600.00 13400.00 0.00 0.08565 19800.00 13400.00 0.00 0.08587 19400.00 13600.00 0.00 0.08682 19600.00 13600.00 0.00 0.08716 19800.00 13600.00 0.00 0.08734 19200.00 13800.00 0.00 0.08737 19400.00 13800.00 0.00 0.08833 19600.00 13800.00 0.00 0.08866 19800.00 13800.00 0.00 0.08886 19200.00 14000.00 0.00 0.08891 19400.00 14000.00 0.00 0.08987 19600.00 14000.00 0.00 0.09023 19800.00 14000.00 0.00 0.09043 19200.00 14200.00 0.00 0.09072 19400.00 14200.00 0.00 0.09168 19600.00 14200.00 0.00 0.09203 19800.00 14200.00 0.00 0.09221 19200.00 14400.00 0.00 0.09242 19400.00 14400.00 0.00 0.09337 19600.00 14400.00 0.00 0.09371 19800.00 14400.00 0.00 0.09387 19200.00 14600.00 0.00 0.09428 19400.00 14600.00 0.00 0.09523 19600.00 14600.00 0.00 0.09557 19800.00 14600.00 0.00 0.09571 19200.00 14800.00 0.00 0.09630 19400.00 14800.00 0.00 0.09726 19600.00 14800.00 0.00 0.09758 19800.00 14800.00 0.00 0.09767 19200.00 15000.00 0.00 0.09837 19400.00 15000.00 0.00 0.09931 19600.00 15000.00 0.00 0.09960 19800.00 15000.00 0.00 0.09964 19200.00 15200.00 0.00 0.10036 19400.00 15200.00 0.00 0.10130 19600.00 15200.00 0.00 0.10156 19200.00 15400.00 0.00 0.10238 19400.00 15400.00 0.00 0.10332 19600.00 15400.00 0.00 0.10355 19200.00 15600.00 0.00 0.10455 19400.00 15600.00 0.00 0.10547 19600.00 15600.00 0.00 0.10563 19200.00 15800.00 0.00 0.10652 19400.00 15800.00 0.00 0.10739 19600.00 15800.00 0.00 0.10747 19200.00 16000.00 0.00 0.10858 19400.00 16000.00 0.00 0.10944 19600.00 16000.00 0.00 0.10945 19200.00 16200.00 0.00 0.11070 19400.00 16200.00 0.00 0.11153 19200.00 16400.00 0.00 0.11289 19400.00 16400.00 0.00 0.11362 19200.00 16600.00 0.00 0.11506 19400.00 16600.00 0.00 0.11569 19000.00 16800.00 0.00 0.11584 19200.00 16800.00 0.00 0.11740 19400.00 16800.00 0.00 0.11797 19000.00 17000.00 0.00 0.11824 19200.00 17000.00 0.00 0.11979 19400.00 17000.00 0.00 0.12035 19000.00 17200.00 0.00 0.12067 19200.00 17200.00 0.00 0.12223 19400.00 17200.00 0.00 0.12275 19000.00 17400.00 0.00 0.12346 19200.00 17400.00 0.00 0.12498 19400.00 17400.00 0.00 0.12538 19000.00 17600.00 0.00 0.12603 19200.00 17600.00 0.00 0.12749 19400.00 17600.00 0.00 0.12777 19000.00 17800.00 0.00 0.12881 19200.00 17800.00 0.00 0.13022 19400.00 17800.00 0.00 0.13040 19000.00 18000.00 0.00 0.13156 19200.00 18000.00 0.00 0.13292 19400.00 18000.00 0.00 0.13292 19000.00 18200.00 0.00 0.13446 19200.00 18200.00 0.00 0.13569 19000.00 18400.00 0.00 0.13745 19200.00 18400.00 0.00 0.13849 19000.00 18600.00 0.00 0.14033 19200.00 18600.00 0.00 0.14108 18800.00 18800.00 0.00 0.14165 19000.00 18800.00 0.00 0.14331 19200.00 18800.00 0.00 0.14361 18800.00 19000.00 0.00 0.14367 19000.00 19000.00 0.00 0.14473 19000.00 19200.00 15.00 0.14479 19000.00 19200.00 20.00 0.14489 19000.00 19200.00 25.00 0.14498 19000.00 19200.00 30.00 0.14506 19000.00 19200.00 35.00 0.14512 19000.00 19200.00 40.00 0.14516 19000.00 19200.00 45.00 0.14519 19000.00 19200.00 50.00 0.14519 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18910.03 19168.52 43.80 0.14540 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/146.ctf NX, NY, NZ: 512 512 1 MODE : real