CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/145.mrc Output diagnostic file name Micrographs_0601/145.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/145.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2532.000 7814.000 Mean, RMS : 4463.350 217.3489 TITLE 1: emi2mrc: 145.emi Sun Jan 05 16:32:20 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 9.688, Y 331.484, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04736 10200.00 10000.00 0.00 0.05007 10400.00 10000.00 0.00 0.05238 10600.00 10000.00 0.00 0.05519 10800.00 10000.00 0.00 0.05809 11000.00 10000.00 0.00 0.06144 11200.00 10000.00 0.00 0.06480 11400.00 10000.00 0.00 0.06788 11600.00 10000.00 0.00 0.07122 11800.00 10000.00 0.00 0.07445 12000.00 10000.00 0.00 0.07791 12200.00 10000.00 0.00 0.08149 12400.00 10000.00 0.00 0.08516 12600.00 10000.00 0.00 0.08881 12800.00 10000.00 0.00 0.09260 13000.00 10000.00 0.00 0.09627 13200.00 10000.00 0.00 0.09973 13400.00 10000.00 0.00 0.10301 13600.00 10000.00 0.00 0.10522 13800.00 10000.00 0.00 0.10640 14000.00 10000.00 0.00 0.10659 13600.00 10200.00 0.00 0.10791 13800.00 10200.00 0.00 0.10899 14000.00 10200.00 0.00 0.10904 13600.00 10400.00 0.00 0.11060 13800.00 10400.00 0.00 0.11159 13600.00 10600.00 0.00 0.11349 13800.00 10600.00 0.00 0.11443 13400.00 10800.00 0.00 0.11449 13600.00 10800.00 0.00 0.11653 13800.00 10800.00 0.00 0.11740 13400.00 11000.00 0.00 0.11790 13600.00 11000.00 0.00 0.11985 13800.00 11000.00 0.00 0.12058 13400.00 11200.00 0.00 0.12096 13600.00 11200.00 0.00 0.12283 13800.00 11200.00 0.00 0.12344 13400.00 11400.00 0.00 0.12417 13600.00 11400.00 0.00 0.12596 13800.00 11400.00 0.00 0.12646 13400.00 11600.00 0.00 0.12738 13600.00 11600.00 0.00 0.12910 13800.00 11600.00 0.00 0.12947 13400.00 11800.00 0.00 0.13083 13600.00 11800.00 0.00 0.13241 13800.00 11800.00 0.00 0.13260 13400.00 12000.00 0.00 0.13429 13600.00 12000.00 0.00 0.13569 13400.00 12200.00 0.00 0.13758 13600.00 12200.00 0.00 0.13883 13400.00 12400.00 0.00 0.14095 13600.00 12400.00 0.00 0.14198 13400.00 12600.00 0.00 0.14393 13600.00 12600.00 0.00 0.14471 13400.00 12800.00 0.00 0.14722 13600.00 12800.00 0.00 0.14759 13400.00 13000.00 0.00 0.14970 13200.00 13200.00 0.00 0.14974 13400.00 13200.00 0.00 0.15210 13200.00 13400.00 0.00 0.15211 13400.00 13400.00 0.00 0.15360 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13367.13 13415.61 18.49 0.15364 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/145.ctf NX, NY, NZ: 512 512 1 MODE : real