CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/143.mrc Output diagnostic file name Micrographs_0601/143.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/143.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2654.000 8126.000 Mean, RMS : 4514.722 224.7711 TITLE 1: emi2mrc: 143.emi Sun Jan 05 16:34:46 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 14.815, Y 327.940, Z -97.762, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00934 10200.00 10000.00 0.00 -0.00932 10400.00 10000.00 0.00 -0.00917 10600.00 10000.00 0.00 -0.00898 10800.00 10000.00 0.00 -0.00890 11000.00 10000.00 0.00 -0.00884 11200.00 10000.00 0.00 -0.00877 11400.00 10000.00 0.00 -0.00869 11600.00 10000.00 0.00 -0.00862 11800.00 10000.00 0.00 -0.00860 12000.00 10000.00 0.00 -0.00854 12200.00 10000.00 0.00 -0.00840 12400.00 10000.00 0.00 -0.00835 12800.00 10000.00 0.00 -0.00824 13000.00 10000.00 0.00 -0.00801 13200.00 10000.00 0.00 -0.00773 13400.00 10000.00 0.00 -0.00740 13600.00 10000.00 0.00 -0.00699 13800.00 10000.00 0.00 -0.00655 14000.00 10000.00 0.00 -0.00611 14200.00 10000.00 0.00 -0.00569 14400.00 10000.00 0.00 -0.00525 14600.00 10000.00 0.00 -0.00473 14800.00 10000.00 0.00 -0.00420 15000.00 10000.00 0.00 -0.00372 15200.00 10000.00 0.00 -0.00325 15400.00 10000.00 0.00 -0.00274 15600.00 10000.00 0.00 -0.00215 15800.00 10000.00 0.00 -0.00160 16000.00 10000.00 0.00 -0.00103 16200.00 10000.00 0.00 -0.00041 16400.00 10000.00 0.00 0.00030 16600.00 10000.00 0.00 0.00122 16800.00 10000.00 0.00 0.00207 17000.00 10000.00 0.00 0.00281 17200.00 10000.00 0.00 0.00358 17400.00 10000.00 0.00 0.00445 17600.00 10000.00 0.00 0.00551 17800.00 10000.00 0.00 0.00655 18000.00 10000.00 0.00 0.00759 18200.00 10000.00 0.00 0.00855 18400.00 10000.00 0.00 0.00931 18600.00 10000.00 0.00 0.01010 18800.00 10000.00 0.00 0.01098 19000.00 10000.00 0.00 0.01194 19200.00 10000.00 0.00 0.01288 19400.00 10000.00 0.00 0.01380 19600.00 10000.00 0.00 0.01485 19800.00 10000.00 0.00 0.01584 20000.00 10000.00 0.00 0.01676 20200.00 10000.00 0.00 0.01784 20400.00 10000.00 0.00 0.01902 20600.00 10000.00 0.00 0.02025 20800.00 10000.00 0.00 0.02139 21000.00 10000.00 0.00 0.02252 21200.00 10000.00 0.00 0.02376 21400.00 10000.00 0.00 0.02496 21600.00 10000.00 0.00 0.02640 21800.00 10000.00 0.00 0.02800 22000.00 10000.00 0.00 0.02949 22200.00 10000.00 0.00 0.03117 22400.00 10000.00 0.00 0.03299 22600.00 10000.00 0.00 0.03488 22800.00 10000.00 0.00 0.03683 23000.00 10000.00 0.00 0.03876 23200.00 10000.00 0.00 0.04050 23400.00 10000.00 0.00 0.04155 23600.00 10000.00 0.00 0.04199 23800.00 10000.00 0.00 0.04209 23600.00 10200.00 0.00 0.04234 23800.00 10200.00 0.00 0.04243 23600.00 10400.00 0.00 0.04272 23800.00 10400.00 0.00 0.04282 23400.00 10600.00 0.00 0.04289 23600.00 10600.00 0.00 0.04330 23800.00 10600.00 0.00 0.04342 23600.00 10800.00 0.00 0.04385 23800.00 10800.00 0.00 0.04399 23600.00 11000.00 0.00 0.04444 23800.00 11000.00 0.00 0.04458 23600.00 11200.00 0.00 0.04503 23800.00 11200.00 0.00 0.04514 23600.00 11400.00 0.00 0.04560 23800.00 11400.00 0.00 0.04568 23400.00 11600.00 0.00 0.04572 23600.00 11600.00 0.00 0.04614 23800.00 11600.00 0.00 0.04622 23400.00 11800.00 0.00 0.04631 23600.00 11800.00 0.00 0.04672 23800.00 11800.00 0.00 0.04683 23400.00 12000.00 0.00 0.04695 23600.00 12000.00 0.00 0.04736 23800.00 12000.00 0.00 0.04747 23400.00 12200.00 0.00 0.04762 23600.00 12200.00 0.00 0.04801 23800.00 12200.00 0.00 0.04809 23400.00 12400.00 0.00 0.04825 23600.00 12400.00 0.00 0.04858 23800.00 12400.00 0.00 0.04866 23400.00 12600.00 0.00 0.04884 23600.00 12600.00 0.00 0.04914 23800.00 12600.00 0.00 0.04922 23400.00 12800.00 0.00 0.04950 23600.00 12800.00 0.00 0.04981 23800.00 12800.00 0.00 0.04988 23400.00 13000.00 0.00 0.05012 23600.00 13000.00 0.00 0.05041 23800.00 13000.00 0.00 0.05047 23400.00 13200.00 0.00 0.05074 23600.00 13200.00 0.00 0.05103 23800.00 13200.00 0.00 0.05110 23400.00 13400.00 0.00 0.05138 23600.00 13400.00 0.00 0.05169 23800.00 13400.00 0.00 0.05181 23400.00 13600.00 0.00 0.05201 23600.00 13600.00 0.00 0.05238 23800.00 13600.00 0.00 0.05252 23400.00 13800.00 0.00 0.05268 23600.00 13800.00 0.00 0.05311 23800.00 13800.00 0.00 0.05327 23400.00 14000.00 0.00 0.05348 23600.00 14000.00 0.00 0.05397 23800.00 14000.00 0.00 0.05410 23400.00 14200.00 0.00 0.05439 23600.00 14200.00 0.00 0.05488 23800.00 14200.00 0.00 0.05497 23400.00 14400.00 0.00 0.05531 23600.00 14400.00 0.00 0.05579 23800.00 14400.00 0.00 0.05586 23400.00 14600.00 0.00 0.05625 23600.00 14600.00 0.00 0.05672 23800.00 14600.00 0.00 0.05676 23400.00 14800.00 0.00 0.05716 23600.00 14800.00 0.00 0.05762 23400.00 15000.00 0.00 0.05815 23600.00 15000.00 0.00 0.05860 23400.00 15200.00 0.00 0.05904 23600.00 15200.00 0.00 0.05944 23400.00 15400.00 0.00 0.06001 23600.00 15400.00 0.00 0.06037 23400.00 15600.00 0.00 0.06118 23600.00 15600.00 0.00 0.06147 23400.00 15800.00 0.00 0.06232 23600.00 15800.00 0.00 0.06256 23200.00 16000.00 0.00 0.06258 23400.00 16000.00 0.00 0.06365 23600.00 16000.00 0.00 0.06387 23200.00 16200.00 0.00 0.06390 23400.00 16200.00 0.00 0.06498 23600.00 16200.00 0.00 0.06519 23200.00 16400.00 0.00 0.06529 23400.00 16400.00 0.00 0.06637 23600.00 16400.00 0.00 0.06657 23200.00 16600.00 0.00 0.06668 23400.00 16600.00 0.00 0.06775 23600.00 16600.00 0.00 0.06792 23200.00 16800.00 0.00 0.06804 23400.00 16800.00 0.00 0.06910 23600.00 16800.00 0.00 0.06924 23200.00 17000.00 0.00 0.06938 23400.00 17000.00 0.00 0.07045 23600.00 17000.00 0.00 0.07060 23200.00 17200.00 0.00 0.07071 23400.00 17200.00 0.00 0.07179 23600.00 17200.00 0.00 0.07192 23200.00 17400.00 0.00 0.07220 23400.00 17400.00 0.00 0.07325 23600.00 17400.00 0.00 0.07332 23200.00 17600.00 0.00 0.07356 23400.00 17600.00 0.00 0.07453 23200.00 17800.00 0.00 0.07498 23400.00 17800.00 0.00 0.07588 23200.00 18000.00 0.00 0.07650 23400.00 18000.00 0.00 0.07739 23600.00 18000.00 0.00 0.07740 23200.00 18200.00 0.00 0.07800 23400.00 18200.00 0.00 0.07894 23600.00 18200.00 0.00 0.07900 23200.00 18400.00 0.00 0.07960 23400.00 18400.00 0.00 0.08060 23600.00 18400.00 0.00 0.08065 23200.00 18600.00 0.00 0.08131 23400.00 18600.00 0.00 0.08233 23600.00 18600.00 0.00 0.08235 23200.00 18800.00 0.00 0.08303 23400.00 18800.00 0.00 0.08406 23200.00 19000.00 0.00 0.08475 23400.00 19000.00 0.00 0.08573 23200.00 19200.00 0.00 0.08662 23400.00 19200.00 0.00 0.08751 23200.00 19400.00 0.00 0.08837 23400.00 19400.00 0.00 0.08924 23200.00 19600.00 0.00 0.09024 23400.00 19600.00 0.00 0.09112 23200.00 19800.00 0.00 0.09211 23400.00 19800.00 0.00 0.09297 23200.00 20000.00 0.00 0.09402 23400.00 20000.00 0.00 0.09488 23200.00 20200.00 0.00 0.09611 23400.00 20200.00 0.00 0.09696 23200.00 20400.00 0.00 0.09817 23400.00 20400.00 0.00 0.09896 23200.00 20600.00 0.00 0.10031 23400.00 20600.00 0.00 0.10105 23200.00 20800.00 0.00 0.10248 23400.00 20800.00 0.00 0.10318 23200.00 21000.00 0.00 0.10480 23400.00 21000.00 0.00 0.10544 23200.00 21200.00 0.00 0.10710 23400.00 21200.00 0.00 0.10767 23200.00 21400.00 0.00 0.10962 23400.00 21400.00 0.00 0.11010 23200.00 21600.00 0.00 0.11226 23400.00 21600.00 0.00 0.11264 23000.00 21800.00 0.00 0.11272 23200.00 21800.00 0.00 0.11492 23400.00 21800.00 0.00 0.11518 23000.00 22000.00 0.00 0.11567 23200.00 22000.00 0.00 0.11783 23400.00 22000.00 0.00 0.11794 23000.00 22200.00 0.00 0.11852 23200.00 22200.00 0.00 0.12061 23000.00 22400.00 0.00 0.12175 23200.00 22400.00 0.00 0.12370 23000.00 22600.00 0.00 0.12506 23200.00 22600.00 0.00 0.12680 23000.00 22800.00 0.00 0.12861 23200.00 22800.00 0.00 0.12989 23000.00 23000.00 0.00 0.13172 23200.00 23000.00 0.00 0.13223 23000.00 23200.00 0.00 0.13277 23000.00 23200.00 5.00 0.13298 23000.00 23200.00 10.00 0.13318 23000.00 23200.00 15.00 0.13336 23000.00 23200.00 20.00 0.13351 23000.00 23200.00 25.00 0.13364 23000.00 23200.00 30.00 0.13373 23000.00 23200.00 35.00 0.13378 23000.00 23200.00 40.00 0.13379 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23000.00 23200.00 38.38 0.13379 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/143.ctf NX, NY, NZ: 512 512 1 MODE : real