CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/142.mrc Output diagnostic file name Micrographs_0601/142.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/142.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2468.000 8444.000 Mean, RMS : 4469.484 216.5634 TITLE 1: emi2mrc: 142.emi Sun Jan 05 16:33:09 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 11.973, Y 327.905, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.12484 10200.00 10000.00 0.00 0.13082 10400.00 10000.00 0.00 0.13594 10600.00 10000.00 0.00 0.14175 10800.00 10000.00 0.00 0.14657 11000.00 10000.00 0.00 0.15001 11200.00 10000.00 0.00 0.15076 10800.00 10200.00 0.00 0.15198 11000.00 10200.00 0.00 0.15516 11200.00 10200.00 0.00 0.15542 10800.00 10400.00 0.00 0.15716 11000.00 10400.00 0.00 0.15989 10800.00 10600.00 0.00 0.16192 11000.00 10600.00 0.00 0.16403 10800.00 10800.00 0.00 0.16459 11000.00 10800.00 0.00 0.16644 10800.00 11000.00 0.00 0.16651 11000.00 11000.00 0.00 0.16766 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10920.06 11036.80 41.38 0.16790 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/142.ctf NX, NY, NZ: 512 512 1 MODE : real