CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/141.mrc Output diagnostic file name Micrographs_0601/141.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/141.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2464.000 9042.000 Mean, RMS : 4459.655 220.8292 TITLE 1: emi2mrc: 141.emi Sun Jan 05 16:31:26 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 12.029, Y 330.815, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02864 10200.00 10000.00 0.00 0.03012 10400.00 10000.00 0.00 0.03158 10600.00 10000.00 0.00 0.03333 10800.00 10000.00 0.00 0.03498 11000.00 10000.00 0.00 0.03677 11200.00 10000.00 0.00 0.03872 11400.00 10000.00 0.00 0.04062 11600.00 10000.00 0.00 0.04268 11800.00 10000.00 0.00 0.04491 12000.00 10000.00 0.00 0.04744 12200.00 10000.00 0.00 0.05009 12400.00 10000.00 0.00 0.05286 12600.00 10000.00 0.00 0.05564 12800.00 10000.00 0.00 0.05849 13000.00 10000.00 0.00 0.06153 13200.00 10000.00 0.00 0.06461 13400.00 10000.00 0.00 0.06778 13600.00 10000.00 0.00 0.07102 13800.00 10000.00 0.00 0.07425 14000.00 10000.00 0.00 0.07744 14200.00 10000.00 0.00 0.08088 14400.00 10000.00 0.00 0.08442 14600.00 10000.00 0.00 0.08799 14800.00 10000.00 0.00 0.09091 15000.00 10000.00 0.00 0.09242 15200.00 10000.00 0.00 0.09295 15000.00 10200.00 0.00 0.09453 15200.00 10200.00 0.00 0.09509 14800.00 10400.00 0.00 0.09513 15000.00 10400.00 0.00 0.09675 15200.00 10400.00 0.00 0.09729 14800.00 10600.00 0.00 0.09756 15000.00 10600.00 0.00 0.09917 15200.00 10600.00 0.00 0.09965 14800.00 10800.00 0.00 0.10016 15000.00 10800.00 0.00 0.10176 15200.00 10800.00 0.00 0.10218 14800.00 11000.00 0.00 0.10292 15000.00 11000.00 0.00 0.10447 15200.00 11000.00 0.00 0.10480 14800.00 11200.00 0.00 0.10550 15000.00 11200.00 0.00 0.10699 15200.00 11200.00 0.00 0.10724 14800.00 11400.00 0.00 0.10839 15000.00 11400.00 0.00 0.10984 15200.00 11400.00 0.00 0.10999 14800.00 11600.00 0.00 0.11134 15000.00 11600.00 0.00 0.11267 14800.00 11800.00 0.00 0.11431 15000.00 11800.00 0.00 0.11549 14800.00 12000.00 0.00 0.11727 15000.00 12000.00 0.00 0.11836 14800.00 12200.00 0.00 0.12038 15000.00 12200.00 0.00 0.12146 14800.00 12400.00 0.00 0.12384 15000.00 12400.00 0.00 0.12486 14800.00 12600.00 0.00 0.12726 15000.00 12600.00 0.00 0.12813 14800.00 12800.00 0.00 0.13095 15000.00 12800.00 0.00 0.13161 14800.00 13000.00 0.00 0.13432 15000.00 13000.00 0.00 0.13486 14600.00 13200.00 0.00 0.13503 14800.00 13200.00 0.00 0.13792 15000.00 13200.00 0.00 0.13833 14600.00 13400.00 0.00 0.13865 14800.00 13400.00 0.00 0.14153 15000.00 13400.00 0.00 0.14176 14600.00 13600.00 0.00 0.14240 14800.00 13600.00 0.00 0.14523 14600.00 13800.00 0.00 0.14637 14800.00 13800.00 0.00 0.14912 14600.00 14000.00 0.00 0.15039 14800.00 14000.00 0.00 0.15306 14600.00 14200.00 0.00 0.15512 14800.00 14200.00 0.00 0.15763 14600.00 14400.00 0.00 0.15934 14800.00 14400.00 0.00 0.16163 14600.00 14600.00 0.00 0.16402 14800.00 14600.00 0.00 0.16556 14600.00 14800.00 0.00 0.16666 14600.00 14800.00 5.00 0.16681 14600.00 14800.00 10.00 0.16695 14600.00 14800.00 15.00 0.16706 14600.00 14800.00 20.00 0.16714 14600.00 14800.00 25.00 0.16720 14600.00 14800.00 30.00 0.16721 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14683.69 14800.00 30.00 0.16764 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/141.ctf NX, NY, NZ: 512 512 1 MODE : real