CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/140.mrc Output diagnostic file name Micrographs_0601/140.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/140.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2276.000 8102.000 Mean, RMS : 4505.637 223.0185 TITLE 1: emi2mrc: 140.emi Sun Jan 05 16:30:05 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.03%, Dif 73.67% TITLE 3: X 14.645, Y 330.610, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01037 16000.00 10000.00 0.00 -0.00999 16200.00 10000.00 0.00 -0.00943 16400.00 10000.00 0.00 -0.00880 16600.00 10000.00 0.00 -0.00814 16800.00 10000.00 0.00 -0.00748 17000.00 10000.00 0.00 -0.00689 17200.00 10000.00 0.00 -0.00624 17400.00 10000.00 0.00 -0.00558 17600.00 10000.00 0.00 -0.00496 17800.00 10000.00 0.00 -0.00435 18000.00 10000.00 0.00 -0.00370 18200.00 10000.00 0.00 -0.00299 18400.00 10000.00 0.00 -0.00231 18600.00 10000.00 0.00 -0.00153 18800.00 10000.00 0.00 -0.00069 19000.00 10000.00 0.00 0.00013 19200.00 10000.00 0.00 0.00114 19400.00 10000.00 0.00 0.00215 19600.00 10000.00 0.00 0.00300 19800.00 10000.00 0.00 0.00382 20000.00 10000.00 0.00 0.00467 20200.00 10000.00 0.00 0.00556 20400.00 10000.00 0.00 0.00649 20600.00 10000.00 0.00 0.00745 20800.00 10000.00 0.00 0.00824 21000.00 10000.00 0.00 0.00904 21200.00 10000.00 0.00 0.01000 21400.00 10000.00 0.00 0.01081 21600.00 10000.00 0.00 0.01168 21800.00 10000.00 0.00 0.01253 22000.00 10000.00 0.00 0.01332 22200.00 10000.00 0.00 0.01420 22400.00 10000.00 0.00 0.01516 22600.00 10000.00 0.00 0.01633 22800.00 10000.00 0.00 0.01751 23000.00 10000.00 0.00 0.01871 23200.00 10000.00 0.00 0.01999 23400.00 10000.00 0.00 0.02141 23600.00 10000.00 0.00 0.02293 23800.00 10000.00 0.00 0.02439 24000.00 10000.00 0.00 0.02599 24200.00 10000.00 0.00 0.02773 24400.00 10000.00 0.00 0.02958 24600.00 10000.00 0.00 0.03148 24800.00 10000.00 0.00 0.03323 25000.00 10000.00 0.00 0.03465 25200.00 10000.00 0.00 0.03558 25400.00 10000.00 0.00 0.03608 25600.00 10000.00 0.00 0.03624 25800.00 10000.00 0.00 0.03627 25400.00 10200.00 0.00 0.03660 25600.00 10200.00 0.00 0.03670 25400.00 10400.00 0.00 0.03692 25600.00 10400.00 0.00 0.03693 25400.00 10600.00 0.00 0.03715 25400.00 10800.00 0.00 0.03742 25600.00 10800.00 0.00 0.03743 25200.00 11000.00 0.00 0.03747 25400.00 11000.00 0.00 0.03777 25600.00 11000.00 0.00 0.03783 25800.00 11000.00 0.00 0.03786 25400.00 11200.00 0.00 0.03811 25600.00 11200.00 0.00 0.03819 25800.00 11200.00 0.00 0.03819 25400.00 11400.00 0.00 0.03848 25600.00 11400.00 0.00 0.03856 25800.00 11400.00 0.00 0.03857 25400.00 11600.00 0.00 0.03883 25600.00 11600.00 0.00 0.03893 25800.00 11600.00 0.00 0.03893 25400.00 11800.00 0.00 0.03927 25600.00 11800.00 0.00 0.03938 25200.00 12000.00 0.00 0.03940 25400.00 12000.00 0.00 0.03972 25600.00 12000.00 0.00 0.03981 25200.00 12200.00 0.00 0.03987 25400.00 12200.00 0.00 0.04018 25600.00 12200.00 0.00 0.04028 25200.00 12400.00 0.00 0.04037 25400.00 12400.00 0.00 0.04071 25600.00 12400.00 0.00 0.04085 25400.00 12600.00 0.00 0.04116 25600.00 12600.00 0.00 0.04131 25400.00 12800.00 0.00 0.04169 25600.00 12800.00 0.00 0.04182 25400.00 13000.00 0.00 0.04220 25600.00 13000.00 0.00 0.04232 25200.00 13200.00 0.00 0.04233 25400.00 13200.00 0.00 0.04279 25600.00 13200.00 0.00 0.04292 25200.00 13400.00 0.00 0.04307 25400.00 13400.00 0.00 0.04357 25600.00 13400.00 0.00 0.04372 25200.00 13600.00 0.00 0.04390 25400.00 13600.00 0.00 0.04444 25600.00 13600.00 0.00 0.04459 25200.00 13800.00 0.00 0.04484 25400.00 13800.00 0.00 0.04538 25600.00 13800.00 0.00 0.04549 25200.00 14000.00 0.00 0.04584 25400.00 14000.00 0.00 0.04633 25600.00 14000.00 0.00 0.04636 25200.00 14200.00 0.00 0.04673 25400.00 14200.00 0.00 0.04717 25200.00 14400.00 0.00 0.04750 25400.00 14400.00 0.00 0.04789 25200.00 14600.00 0.00 0.04831 25400.00 14600.00 0.00 0.04863 25200.00 14800.00 0.00 0.04906 25400.00 14800.00 0.00 0.04935 25200.00 15000.00 0.00 0.04988 25400.00 15000.00 0.00 0.05015 25200.00 15200.00 0.00 0.05064 25400.00 15200.00 0.00 0.05088 25200.00 15400.00 0.00 0.05131 25400.00 15400.00 0.00 0.05150 25200.00 15600.00 0.00 0.05196 25400.00 15600.00 0.00 0.05210 25200.00 15800.00 0.00 0.05261 25400.00 15800.00 0.00 0.05275 25000.00 16000.00 0.00 0.05281 25200.00 16000.00 0.00 0.05343 25400.00 16000.00 0.00 0.05356 25200.00 16200.00 0.00 0.05412 25400.00 16200.00 0.00 0.05426 25000.00 16400.00 0.00 0.05435 25200.00 16400.00 0.00 0.05500 25400.00 16400.00 0.00 0.05519 25000.00 16600.00 0.00 0.05550 25200.00 16600.00 0.00 0.05617 25400.00 16600.00 0.00 0.05632 25000.00 16800.00 0.00 0.05658 25200.00 16800.00 0.00 0.05719 25400.00 16800.00 0.00 0.05729 25000.00 17000.00 0.00 0.05757 25200.00 17000.00 0.00 0.05819 25400.00 17000.00 0.00 0.05832 25000.00 17200.00 0.00 0.05860 25200.00 17200.00 0.00 0.05926 25400.00 17200.00 0.00 0.05940 25000.00 17400.00 0.00 0.05970 25200.00 17400.00 0.00 0.06033 25400.00 17400.00 0.00 0.06044 25000.00 17600.00 0.00 0.06076 25200.00 17600.00 0.00 0.06138 25400.00 17600.00 0.00 0.06150 25000.00 17800.00 0.00 0.06185 25200.00 17800.00 0.00 0.06251 25400.00 17800.00 0.00 0.06268 25000.00 18000.00 0.00 0.06300 25200.00 18000.00 0.00 0.06372 25400.00 18000.00 0.00 0.06390 25000.00 18200.00 0.00 0.06424 25200.00 18200.00 0.00 0.06498 25400.00 18200.00 0.00 0.06514 25000.00 18400.00 0.00 0.06552 25200.00 18400.00 0.00 0.06625 25400.00 18400.00 0.00 0.06638 25000.00 18600.00 0.00 0.06677 25200.00 18600.00 0.00 0.06750 25400.00 18600.00 0.00 0.06762 25000.00 18800.00 0.00 0.06811 25200.00 18800.00 0.00 0.06887 25400.00 18800.00 0.00 0.06899 25000.00 19000.00 0.00 0.06961 25200.00 19000.00 0.00 0.07037 25400.00 19000.00 0.00 0.07043 25000.00 19200.00 0.00 0.07118 25200.00 19200.00 0.00 0.07188 25000.00 19400.00 0.00 0.07271 25200.00 19400.00 0.00 0.07335 25000.00 19600.00 0.00 0.07425 25200.00 19600.00 0.00 0.07486 25000.00 19800.00 0.00 0.07564 25200.00 19800.00 0.00 0.07624 25000.00 20000.00 0.00 0.07709 25200.00 20000.00 0.00 0.07768 25000.00 20200.00 0.00 0.07868 25200.00 20200.00 0.00 0.07922 25000.00 20400.00 0.00 0.08018 25200.00 20400.00 0.00 0.08066 25000.00 20600.00 0.00 0.08178 25200.00 20600.00 0.00 0.08217 25000.00 20800.00 0.00 0.08339 25200.00 20800.00 0.00 0.08371 25000.00 21000.00 0.00 0.08500 25200.00 21000.00 0.00 0.08526 25000.00 21200.00 0.00 0.08648 25200.00 21200.00 0.00 0.08674 25000.00 21400.00 0.00 0.08807 25200.00 21400.00 0.00 0.08834 24800.00 21600.00 0.00 0.08846 25000.00 21600.00 0.00 0.08979 25200.00 21600.00 0.00 0.09003 24800.00 21800.00 0.00 0.09020 25000.00 21800.00 0.00 0.09149 25200.00 21800.00 0.00 0.09169 24800.00 22000.00 0.00 0.09201 25000.00 22000.00 0.00 0.09326 25200.00 22000.00 0.00 0.09341 24800.00 22200.00 0.00 0.09379 25000.00 22200.00 0.00 0.09502 25200.00 22200.00 0.00 0.09512 24800.00 22400.00 0.00 0.09571 25000.00 22400.00 0.00 0.09690 25200.00 22400.00 0.00 0.09691 24800.00 22600.00 0.00 0.09757 25000.00 22600.00 0.00 0.09871 24800.00 22800.00 0.00 0.09956 25000.00 22800.00 0.00 0.10065 24800.00 23000.00 0.00 0.10166 25000.00 23000.00 0.00 0.10267 24800.00 23200.00 0.00 0.10373 25000.00 23200.00 0.00 0.10464 24800.00 23400.00 0.00 0.10575 25000.00 23400.00 0.00 0.10653 24800.00 23600.00 0.00 0.10770 25000.00 23600.00 0.00 0.10838 24800.00 23800.00 0.00 0.10987 25000.00 23800.00 0.00 0.11043 24800.00 24000.00 0.00 0.11192 25000.00 24000.00 0.00 0.11233 24800.00 24200.00 0.00 0.11417 25000.00 24200.00 0.00 0.11436 24600.00 24400.00 0.00 0.11443 24800.00 24400.00 0.00 0.11629 24600.00 24600.00 0.00 0.11648 24800.00 24600.00 0.00 0.11817 24600.00 24800.00 0.00 0.11818 24800.00 24800.00 0.00 0.11939 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24739.18 24894.65 36.75 0.11967 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/140.ctf NX, NY, NZ: 512 512 1 MODE : real