CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/138.mrc Output diagnostic file name Micrographs_0601/138.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/138.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2336.000 8292.000 Mean, RMS : 4472.518 217.0409 TITLE 1: emi2mrc: 138.emi Sun Jan 05 16:13:40 2014 TITLE 2: kV 300, Mag 78000x, Def 18210, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 23.214, Y 334.699, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.11328 10200.00 10000.00 0.00 0.11860 10400.00 10000.00 0.00 0.12350 10600.00 10000.00 0.00 0.12942 10800.00 10000.00 0.00 0.13510 11000.00 10000.00 0.00 0.14133 11200.00 10000.00 0.00 0.14593 11400.00 10000.00 0.00 0.14655 11000.00 10200.00 0.00 0.14673 11200.00 10200.00 0.00 0.15129 11400.00 10200.00 0.00 0.15154 11000.00 10400.00 0.00 0.15241 11200.00 10400.00 0.00 0.15684 11000.00 10600.00 0.00 0.15835 11200.00 10600.00 0.00 0.16247 11000.00 10800.00 0.00 0.16395 11200.00 10800.00 0.00 0.16768 11000.00 11000.00 0.00 0.17088 11200.00 11000.00 0.00 0.17332 11000.00 11200.00 0.00 0.17506 11200.00 11200.00 0.00 0.17513 11000.00 11200.00 5.00 0.17514 11000.00 11200.00 10.00 0.17518 11000.00 11200.00 15.00 0.17520 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 11089.87 11216.41 13.56 0.17622 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/138.ctf NX, NY, NZ: 512 512 1 MODE : real