CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/137.mrc Output diagnostic file name Micrographs_0601/137.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/137.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2416.000 7800.000 Mean, RMS : 4517.575 222.2069 TITLE 1: emi2mrc: 137.emi Sun Jan 05 16:27:28 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.03%, Dif 73.67% TITLE 3: X 22.681, Y 329.154, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00221 10200.00 10000.00 0.00 -0.00177 10400.00 10000.00 0.00 -0.00145 10600.00 10000.00 0.00 -0.00119 10800.00 10000.00 0.00 -0.00094 11000.00 10000.00 0.00 -0.00060 11200.00 10000.00 0.00 -0.00014 11400.00 10000.00 0.00 0.00036 11600.00 10000.00 0.00 0.00084 11800.00 10000.00 0.00 0.00136 12000.00 10000.00 0.00 0.00189 12200.00 10000.00 0.00 0.00229 12400.00 10000.00 0.00 0.00267 12600.00 10000.00 0.00 0.00310 12800.00 10000.00 0.00 0.00358 13000.00 10000.00 0.00 0.00423 13200.00 10000.00 0.00 0.00498 13400.00 10000.00 0.00 0.00579 13600.00 10000.00 0.00 0.00659 13800.00 10000.00 0.00 0.00736 14000.00 10000.00 0.00 0.00814 14200.00 10000.00 0.00 0.00890 14400.00 10000.00 0.00 0.00968 14600.00 10000.00 0.00 0.01077 14800.00 10000.00 0.00 0.01203 15000.00 10000.00 0.00 0.01328 15200.00 10000.00 0.00 0.01459 15400.00 10000.00 0.00 0.01572 15600.00 10000.00 0.00 0.01675 15800.00 10000.00 0.00 0.01789 16000.00 10000.00 0.00 0.01907 16200.00 10000.00 0.00 0.02024 16400.00 10000.00 0.00 0.02143 16600.00 10000.00 0.00 0.02279 16800.00 10000.00 0.00 0.02427 17000.00 10000.00 0.00 0.02583 17200.00 10000.00 0.00 0.02755 17400.00 10000.00 0.00 0.02921 17600.00 10000.00 0.00 0.03070 17800.00 10000.00 0.00 0.03226 18000.00 10000.00 0.00 0.03398 18200.00 10000.00 0.00 0.03575 18400.00 10000.00 0.00 0.03764 18600.00 10000.00 0.00 0.03963 18800.00 10000.00 0.00 0.04175 19000.00 10000.00 0.00 0.04378 19200.00 10000.00 0.00 0.04571 19400.00 10000.00 0.00 0.04784 19600.00 10000.00 0.00 0.04995 19800.00 10000.00 0.00 0.05198 20000.00 10000.00 0.00 0.05379 20200.00 10000.00 0.00 0.05493 20400.00 10000.00 0.00 0.05541 20600.00 10000.00 0.00 0.05562 21000.00 10000.00 0.00 0.05571 21200.00 10000.00 0.00 0.05585 20400.00 10200.00 0.00 0.05613 20600.00 10200.00 0.00 0.05636 20800.00 10200.00 0.00 0.05641 21000.00 10200.00 0.00 0.05654 21200.00 10200.00 0.00 0.05668 20400.00 10400.00 0.00 0.05684 20600.00 10400.00 0.00 0.05711 20800.00 10400.00 0.00 0.05723 21000.00 10400.00 0.00 0.05742 21200.00 10400.00 0.00 0.05756 20400.00 10600.00 0.00 0.05765 20600.00 10600.00 0.00 0.05798 20800.00 10600.00 0.00 0.05815 21000.00 10600.00 0.00 0.05836 21200.00 10600.00 0.00 0.05847 20400.00 10800.00 0.00 0.05863 20600.00 10800.00 0.00 0.05899 20800.00 10800.00 0.00 0.05917 21000.00 10800.00 0.00 0.05938 21200.00 10800.00 0.00 0.05945 20400.00 11000.00 0.00 0.05964 20600.00 11000.00 0.00 0.05999 20800.00 11000.00 0.00 0.06017 21000.00 11000.00 0.00 0.06037 21200.00 11000.00 0.00 0.06040 20400.00 11200.00 0.00 0.06064 20600.00 11200.00 0.00 0.06096 20800.00 11200.00 0.00 0.06115 21000.00 11200.00 0.00 0.06135 20400.00 11400.00 0.00 0.06187 20600.00 11400.00 0.00 0.06216 20800.00 11400.00 0.00 0.06237 21000.00 11400.00 0.00 0.06256 20400.00 11600.00 0.00 0.06293 20600.00 11600.00 0.00 0.06321 20800.00 11600.00 0.00 0.06344 21000.00 11600.00 0.00 0.06360 20400.00 11800.00 0.00 0.06393 20600.00 11800.00 0.00 0.06425 20800.00 11800.00 0.00 0.06452 21000.00 11800.00 0.00 0.06464 20400.00 12000.00 0.00 0.06509 20600.00 12000.00 0.00 0.06543 20800.00 12000.00 0.00 0.06569 21000.00 12000.00 0.00 0.06574 20400.00 12200.00 0.00 0.06618 20600.00 12200.00 0.00 0.06650 20800.00 12200.00 0.00 0.06670 20200.00 12400.00 0.00 0.06676 20400.00 12400.00 0.00 0.06731 20600.00 12400.00 0.00 0.06760 20800.00 12400.00 0.00 0.06773 20200.00 12600.00 0.00 0.06791 20400.00 12600.00 0.00 0.06839 20600.00 12600.00 0.00 0.06861 20800.00 12600.00 0.00 0.06868 20200.00 12800.00 0.00 0.06894 20400.00 12800.00 0.00 0.06936 20600.00 12800.00 0.00 0.06955 20800.00 12800.00 0.00 0.06960 20200.00 13000.00 0.00 0.06993 20400.00 13000.00 0.00 0.07036 20600.00 13000.00 0.00 0.07057 20800.00 13000.00 0.00 0.07064 20200.00 13200.00 0.00 0.07114 20400.00 13200.00 0.00 0.07160 20600.00 13200.00 0.00 0.07183 20800.00 13200.00 0.00 0.07187 20200.00 13400.00 0.00 0.07245 20400.00 13400.00 0.00 0.07289 20600.00 13400.00 0.00 0.07307 20800.00 13400.00 0.00 0.07307 20200.00 13600.00 0.00 0.07380 20400.00 13600.00 0.00 0.07418 20600.00 13600.00 0.00 0.07434 20200.00 13800.00 0.00 0.07515 20400.00 13800.00 0.00 0.07552 20600.00 13800.00 0.00 0.07567 20800.00 13800.00 0.00 0.07569 20200.00 14000.00 0.00 0.07647 20400.00 14000.00 0.00 0.07685 20600.00 14000.00 0.00 0.07704 20800.00 14000.00 0.00 0.07708 20200.00 14200.00 0.00 0.07799 20400.00 14200.00 0.00 0.07838 20600.00 14200.00 0.00 0.07858 20800.00 14200.00 0.00 0.07864 20200.00 14400.00 0.00 0.07952 20400.00 14400.00 0.00 0.07991 20600.00 14400.00 0.00 0.08011 20800.00 14400.00 0.00 0.08016 20200.00 14600.00 0.00 0.08118 20400.00 14600.00 0.00 0.08157 20600.00 14600.00 0.00 0.08179 20800.00 14600.00 0.00 0.08181 20200.00 14800.00 0.00 0.08279 20400.00 14800.00 0.00 0.08321 20600.00 14800.00 0.00 0.08343 20200.00 15000.00 0.00 0.08441 20400.00 15000.00 0.00 0.08484 20600.00 15000.00 0.00 0.08502 20200.00 15200.00 0.00 0.08619 20400.00 15200.00 0.00 0.08660 20600.00 15200.00 0.00 0.08674 20000.00 15400.00 0.00 0.08684 20200.00 15400.00 0.00 0.08803 20400.00 15400.00 0.00 0.08842 20600.00 15400.00 0.00 0.08848 20000.00 15600.00 0.00 0.08887 20200.00 15600.00 0.00 0.09004 20400.00 15600.00 0.00 0.09034 20000.00 15800.00 0.00 0.09083 20200.00 15800.00 0.00 0.09193 20400.00 15800.00 0.00 0.09214 20000.00 16000.00 0.00 0.09274 20200.00 16000.00 0.00 0.09380 20400.00 16000.00 0.00 0.09398 20000.00 16200.00 0.00 0.09477 20200.00 16200.00 0.00 0.09582 20400.00 16200.00 0.00 0.09599 20000.00 16400.00 0.00 0.09689 20200.00 16400.00 0.00 0.09790 20400.00 16400.00 0.00 0.09802 20000.00 16600.00 0.00 0.09890 20200.00 16600.00 0.00 0.09986 20400.00 16600.00 0.00 0.09992 20000.00 16800.00 0.00 0.10102 20200.00 16800.00 0.00 0.10194 20400.00 16800.00 0.00 0.10195 20000.00 17000.00 0.00 0.10311 20200.00 17000.00 0.00 0.10401 20000.00 17200.00 0.00 0.10508 20200.00 17200.00 0.00 0.10599 20000.00 17400.00 0.00 0.10750 20200.00 17400.00 0.00 0.10840 20000.00 17600.00 0.00 0.10998 20200.00 17600.00 0.00 0.11081 20000.00 17800.00 0.00 0.11239 20200.00 17800.00 0.00 0.11315 20000.00 18000.00 0.00 0.11476 20200.00 18000.00 0.00 0.11547 20000.00 18200.00 0.00 0.11720 20200.00 18200.00 0.00 0.11784 20000.00 18400.00 0.00 0.11968 20200.00 18400.00 0.00 0.12023 19800.00 18600.00 0.00 0.12045 20000.00 18600.00 0.00 0.12231 20200.00 18600.00 0.00 0.12272 19800.00 18800.00 0.00 0.12331 20000.00 18800.00 0.00 0.12508 20200.00 18800.00 0.00 0.12533 19800.00 19000.00 0.00 0.12620 20000.00 19000.00 0.00 0.12787 20200.00 19000.00 0.00 0.12796 19800.00 19200.00 0.00 0.12958 20000.00 19200.00 0.00 0.13109 19800.00 19400.00 0.00 0.13259 20000.00 19400.00 0.00 0.13388 19800.00 19600.00 0.00 0.13569 20000.00 19600.00 0.00 0.13661 19800.00 19800.00 0.00 0.13834 20000.00 19800.00 0.00 0.13859 19800.00 20000.00 0.00 0.13876 19800.00 20000.00 5.00 0.13889 19800.00 20000.00 10.00 0.13902 19800.00 20000.00 15.00 0.13913 19800.00 20000.00 20.00 0.13924 19800.00 20000.00 25.00 0.13933 19800.00 20000.00 30.00 0.13940 19800.00 20000.00 35.00 0.13944 19800.00 20000.00 40.00 0.13946 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19782.34 20007.79 40.00 0.13947 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/137.ctf NX, NY, NZ: 512 512 1 MODE : real