CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/133.mrc Output diagnostic file name Micrographs_0601/133.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/133.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2466.000 8090.000 Mean, RMS : 4463.716 213.9116 TITLE 1: emi2mrc: 133.emi Sun Jan 05 16:21:07 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 10.204, Y 333.399, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04089 10200.00 10000.00 0.00 0.04309 10400.00 10000.00 0.00 0.04518 10600.00 10000.00 0.00 0.04778 10800.00 10000.00 0.00 0.05024 11000.00 10000.00 0.00 0.05282 11200.00 10000.00 0.00 0.05566 11400.00 10000.00 0.00 0.05872 11600.00 10000.00 0.00 0.06209 11800.00 10000.00 0.00 0.06537 12000.00 10000.00 0.00 0.06882 12200.00 10000.00 0.00 0.07236 12400.00 10000.00 0.00 0.07605 12600.00 10000.00 0.00 0.07996 12800.00 10000.00 0.00 0.08389 13000.00 10000.00 0.00 0.08779 13200.00 10000.00 0.00 0.09213 13400.00 10000.00 0.00 0.09667 13600.00 10000.00 0.00 0.10034 13800.00 10000.00 0.00 0.10264 14000.00 10000.00 0.00 0.10302 13800.00 10200.00 0.00 0.10520 14000.00 10200.00 0.00 0.10553 13600.00 10400.00 0.00 0.10560 13800.00 10400.00 0.00 0.10780 14000.00 10400.00 0.00 0.10806 13600.00 10600.00 0.00 0.10837 13800.00 10600.00 0.00 0.11057 14000.00 10600.00 0.00 0.11078 13600.00 10800.00 0.00 0.11112 13800.00 10800.00 0.00 0.11333 14000.00 10800.00 0.00 0.11346 13600.00 11000.00 0.00 0.11431 13800.00 11000.00 0.00 0.11648 14000.00 11000.00 0.00 0.11648 13600.00 11200.00 0.00 0.11746 13800.00 11200.00 0.00 0.11953 13600.00 11400.00 0.00 0.12075 13800.00 11400.00 0.00 0.12274 13600.00 11600.00 0.00 0.12402 13800.00 11600.00 0.00 0.12592 13600.00 11800.00 0.00 0.12733 13800.00 11800.00 0.00 0.12914 13600.00 12000.00 0.00 0.13060 13800.00 12000.00 0.00 0.13237 13600.00 12200.00 0.00 0.13392 13800.00 12200.00 0.00 0.13561 13600.00 12400.00 0.00 0.13722 13800.00 12400.00 0.00 0.13876 13600.00 12600.00 0.00 0.14014 13800.00 12600.00 0.00 0.14149 13600.00 12800.00 0.00 0.14339 13800.00 12800.00 0.00 0.14444 13600.00 13000.00 0.00 0.14599 13800.00 13000.00 0.00 0.14673 13600.00 13200.00 0.00 0.14852 13800.00 13200.00 0.00 0.14887 13600.00 13400.00 0.00 0.15053 13600.00 13600.00 0.00 0.15207 13600.00 13800.00 0.00 0.15304 13600.00 13800.00 5.00 0.15316 13600.00 13800.00 10.00 0.15326 13600.00 13800.00 15.00 0.15332 13600.00 13800.00 20.00 0.15336 13600.00 13800.00 25.00 0.15336 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13582.81 13800.00 23.54 0.15337 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/133.ctf NX, NY, NZ: 512 512 1 MODE : real