CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/132.mrc Output diagnostic file name Micrographs_0601/132.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/132.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2472.000 7416.000 Mean, RMS : 4489.425 219.2137 TITLE 1: emi2mrc: 132.emi Sun Jan 05 16:24:33 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 20.222, Y 332.185, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03775 10200.00 10000.00 0.00 0.03957 10400.00 10000.00 0.00 0.04109 10600.00 10000.00 0.00 0.04302 10800.00 10000.00 0.00 0.04510 11000.00 10000.00 0.00 0.04728 11200.00 10000.00 0.00 0.04954 11400.00 10000.00 0.00 0.05193 11600.00 10000.00 0.00 0.05439 11800.00 10000.00 0.00 0.05697 12000.00 10000.00 0.00 0.06000 12200.00 10000.00 0.00 0.06285 12400.00 10000.00 0.00 0.06573 12600.00 10000.00 0.00 0.06892 12800.00 10000.00 0.00 0.07226 13000.00 10000.00 0.00 0.07551 13200.00 10000.00 0.00 0.07875 13400.00 10000.00 0.00 0.08225 13600.00 10000.00 0.00 0.08589 13800.00 10000.00 0.00 0.08980 14000.00 10000.00 0.00 0.09389 14200.00 10000.00 0.00 0.09775 14400.00 10000.00 0.00 0.10016 14600.00 10000.00 0.00 0.10092 14400.00 10200.00 0.00 0.10235 14600.00 10200.00 0.00 0.10300 14400.00 10400.00 0.00 0.10468 14600.00 10400.00 0.00 0.10525 14400.00 10600.00 0.00 0.10720 14600.00 10600.00 0.00 0.10762 14400.00 10800.00 0.00 0.10968 14600.00 10800.00 0.00 0.10994 14200.00 11000.00 0.00 0.11027 14400.00 11000.00 0.00 0.11249 14600.00 11000.00 0.00 0.11263 14200.00 11200.00 0.00 0.11319 14400.00 11200.00 0.00 0.11531 14600.00 11200.00 0.00 0.11536 14200.00 11400.00 0.00 0.11638 14400.00 11400.00 0.00 0.11840 14600.00 11400.00 0.00 0.11840 14200.00 11600.00 0.00 0.11946 14400.00 11600.00 0.00 0.12142 14600.00 11600.00 0.00 0.12143 14200.00 11800.00 0.00 0.12276 14400.00 11800.00 0.00 0.12471 14200.00 12000.00 0.00 0.12610 14400.00 12000.00 0.00 0.12807 14200.00 12200.00 0.00 0.12950 14400.00 12200.00 0.00 0.13146 14200.00 12400.00 0.00 0.13335 14400.00 12400.00 0.00 0.13526 14200.00 12600.00 0.00 0.13703 14400.00 12600.00 0.00 0.13887 14200.00 12800.00 0.00 0.14089 14400.00 12800.00 0.00 0.14264 14200.00 13000.00 0.00 0.14464 14400.00 13000.00 0.00 0.14625 14200.00 13200.00 0.00 0.14871 14400.00 13200.00 0.00 0.15009 14200.00 13400.00 0.00 0.15265 14400.00 13400.00 0.00 0.15374 14200.00 13600.00 0.00 0.15678 14400.00 13600.00 0.00 0.15750 14200.00 13800.00 0.00 0.16103 14400.00 13800.00 0.00 0.16113 14200.00 14000.00 0.00 0.16473 14000.00 14200.00 0.00 0.16511 14200.00 14200.00 0.00 0.16811 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14299.66 14171.04 -59.94 0.16878 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/132.ctf NX, NY, NZ: 512 512 1 MODE : real