CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/128.mrc Output diagnostic file name Micrographs_0601/128.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/128.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2414.000 7602.000 Mean, RMS : 4423.259 218.0226 TITLE 1: emi2mrc: 128.emi Sun Jan 05 16:19:44 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 5.081, Y 337.656, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02553 10200.00 10000.00 0.00 0.02704 10400.00 10000.00 0.00 0.02859 10600.00 10000.00 0.00 0.03032 10800.00 10000.00 0.00 0.03187 11000.00 10000.00 0.00 0.03354 11200.00 10000.00 0.00 0.03546 11400.00 10000.00 0.00 0.03754 11600.00 10000.00 0.00 0.03971 11800.00 10000.00 0.00 0.04177 12000.00 10000.00 0.00 0.04404 12200.00 10000.00 0.00 0.04632 12400.00 10000.00 0.00 0.04864 12600.00 10000.00 0.00 0.05114 12800.00 10000.00 0.00 0.05375 13000.00 10000.00 0.00 0.05636 13200.00 10000.00 0.00 0.05904 13400.00 10000.00 0.00 0.06176 13600.00 10000.00 0.00 0.06438 13800.00 10000.00 0.00 0.06716 14000.00 10000.00 0.00 0.06985 14200.00 10000.00 0.00 0.07245 14400.00 10000.00 0.00 0.07512 14600.00 10000.00 0.00 0.07783 14800.00 10000.00 0.00 0.08052 15000.00 10000.00 0.00 0.08276 15200.00 10000.00 0.00 0.08431 15400.00 10000.00 0.00 0.08522 15600.00 10000.00 0.00 0.08566 15800.00 10000.00 0.00 0.08567 15200.00 10200.00 0.00 0.08613 15400.00 10200.00 0.00 0.08698 15600.00 10200.00 0.00 0.08737 15200.00 10400.00 0.00 0.08806 15400.00 10400.00 0.00 0.08891 15600.00 10400.00 0.00 0.08929 15200.00 10600.00 0.00 0.09018 15400.00 10600.00 0.00 0.09105 15600.00 10600.00 0.00 0.09140 15200.00 10800.00 0.00 0.09232 15400.00 10800.00 0.00 0.09316 15600.00 10800.00 0.00 0.09340 15200.00 11000.00 0.00 0.09463 15400.00 11000.00 0.00 0.09535 15600.00 11000.00 0.00 0.09547 15200.00 11200.00 0.00 0.09681 15400.00 11200.00 0.00 0.09743 15600.00 11200.00 0.00 0.09749 15000.00 11400.00 0.00 0.09788 15200.00 11400.00 0.00 0.09921 15400.00 11400.00 0.00 0.09981 15600.00 11400.00 0.00 0.09988 15000.00 11600.00 0.00 0.10025 15200.00 11600.00 0.00 0.10161 15400.00 11600.00 0.00 0.10222 15600.00 11600.00 0.00 0.10226 15000.00 11800.00 0.00 0.10286 15200.00 11800.00 0.00 0.10425 15400.00 11800.00 0.00 0.10484 15000.00 12000.00 0.00 0.10572 15200.00 12000.00 0.00 0.10712 15400.00 12000.00 0.00 0.10765 15000.00 12200.00 0.00 0.10855 15200.00 12200.00 0.00 0.10991 15400.00 12200.00 0.00 0.11034 15000.00 12400.00 0.00 0.11133 15200.00 12400.00 0.00 0.11263 15400.00 12400.00 0.00 0.11296 15000.00 12600.00 0.00 0.11386 15200.00 12600.00 0.00 0.11512 15400.00 12600.00 0.00 0.11538 15000.00 12800.00 0.00 0.11667 15200.00 12800.00 0.00 0.11789 15400.00 12800.00 0.00 0.11809 15000.00 13000.00 0.00 0.11942 15200.00 13000.00 0.00 0.12064 15400.00 13000.00 0.00 0.12076 15000.00 13200.00 0.00 0.12248 15200.00 13200.00 0.00 0.12368 15400.00 13200.00 0.00 0.12368 15000.00 13400.00 0.00 0.12556 15200.00 13400.00 0.00 0.12669 14800.00 13600.00 0.00 0.12675 15000.00 13600.00 0.00 0.12868 15200.00 13600.00 0.00 0.12968 14800.00 13800.00 0.00 0.13001 15000.00 13800.00 0.00 0.13183 15200.00 13800.00 0.00 0.13267 14800.00 14000.00 0.00 0.13288 15000.00 14000.00 0.00 0.13456 15200.00 14000.00 0.00 0.13521 14800.00 14200.00 0.00 0.13586 15000.00 14200.00 0.00 0.13732 15200.00 14200.00 0.00 0.13773 14800.00 14400.00 0.00 0.13839 15000.00 14400.00 0.00 0.13964 15200.00 14400.00 0.00 0.13979 14800.00 14600.00 0.00 0.14130 15000.00 14600.00 0.00 0.14213 14800.00 14800.00 0.00 0.14342 15000.00 14800.00 0.00 0.14377 14800.00 15000.00 0.00 0.14431 15000.00 15000.00 0.00 0.14462 14800.00 15200.00 5.00 0.14468 14800.00 15200.00 10.00 0.14473 14800.00 15200.00 15.00 0.14475 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14800.00 15130.00 22.19 0.14486 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/128.ctf NX, NY, NZ: 512 512 1 MODE : real