CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/125.mrc Output diagnostic file name Micrographs_0601/125.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/125.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2496.000 8128.000 Mean, RMS : 4463.374 218.7718 TITLE 1: emi2mrc: 125.emi Sun Jan 05 16:17:18 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 12.471, Y 336.289, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02541 10200.00 10000.00 0.00 0.02649 10400.00 10000.00 0.00 0.02763 10600.00 10000.00 0.00 0.02904 10800.00 10000.00 0.00 0.03049 11000.00 10000.00 0.00 0.03218 11200.00 10000.00 0.00 0.03400 11400.00 10000.00 0.00 0.03594 11600.00 10000.00 0.00 0.03809 11800.00 10000.00 0.00 0.04038 12000.00 10000.00 0.00 0.04277 12200.00 10000.00 0.00 0.04511 12400.00 10000.00 0.00 0.04769 12600.00 10000.00 0.00 0.05023 12800.00 10000.00 0.00 0.05280 13000.00 10000.00 0.00 0.05549 13200.00 10000.00 0.00 0.05830 13400.00 10000.00 0.00 0.06130 13600.00 10000.00 0.00 0.06410 13800.00 10000.00 0.00 0.06709 14000.00 10000.00 0.00 0.07018 14200.00 10000.00 0.00 0.07353 14400.00 10000.00 0.00 0.07702 14600.00 10000.00 0.00 0.08047 14800.00 10000.00 0.00 0.08409 15000.00 10000.00 0.00 0.08762 15200.00 10000.00 0.00 0.09044 15400.00 10000.00 0.00 0.09160 15600.00 10000.00 0.00 0.09185 15200.00 10200.00 0.00 0.09219 15400.00 10200.00 0.00 0.09331 15600.00 10200.00 0.00 0.09353 15200.00 10400.00 0.00 0.09404 15400.00 10400.00 0.00 0.09518 15600.00 10400.00 0.00 0.09540 15200.00 10600.00 0.00 0.09608 15400.00 10600.00 0.00 0.09722 15600.00 10600.00 0.00 0.09739 15200.00 10800.00 0.00 0.09819 15400.00 10800.00 0.00 0.09927 15600.00 10800.00 0.00 0.09935 15200.00 11000.00 0.00 0.10059 15400.00 11000.00 0.00 0.10160 15200.00 11200.00 0.00 0.10291 15400.00 11200.00 0.00 0.10384 15200.00 11400.00 0.00 0.10542 15400.00 11400.00 0.00 0.10625 15200.00 11600.00 0.00 0.10787 15400.00 11600.00 0.00 0.10858 15200.00 11800.00 0.00 0.11046 15400.00 11800.00 0.00 0.11105 15200.00 12000.00 0.00 0.11312 15400.00 12000.00 0.00 0.11364 15200.00 12200.00 0.00 0.11588 15400.00 12200.00 0.00 0.11638 15200.00 12400.00 0.00 0.11893 15400.00 12400.00 0.00 0.11940 15200.00 12600.00 0.00 0.12188 15400.00 12600.00 0.00 0.12229 15000.00 12800.00 0.00 0.12248 15200.00 12800.00 0.00 0.12516 15400.00 12800.00 0.00 0.12549 15000.00 13000.00 0.00 0.12564 15200.00 13000.00 0.00 0.12830 15400.00 13000.00 0.00 0.12853 15000.00 13200.00 0.00 0.12897 15200.00 13200.00 0.00 0.13160 15400.00 13200.00 0.00 0.13169 15000.00 13400.00 0.00 0.13234 15200.00 13400.00 0.00 0.13492 15000.00 13600.00 0.00 0.13575 15200.00 13600.00 0.00 0.13827 15000.00 13800.00 0.00 0.13923 15200.00 13800.00 0.00 0.14167 15000.00 14000.00 0.00 0.14260 15200.00 14000.00 0.00 0.14490 15000.00 14200.00 0.00 0.14638 15200.00 14200.00 0.00 0.14847 15000.00 14400.00 0.00 0.14985 15200.00 14400.00 0.00 0.15167 15000.00 14600.00 0.00 0.15349 15200.00 14600.00 0.00 0.15486 15000.00 14800.00 0.00 0.15637 15200.00 14800.00 0.00 0.15725 15000.00 15000.00 0.00 0.15909 15200.00 15000.00 0.00 0.15924 15000.00 15200.00 0.00 0.16062 15000.00 15200.00 5.00 0.16074 15000.00 15200.00 10.00 0.16083 15000.00 15200.00 15.00 0.16091 15000.00 15200.00 20.00 0.16095 15000.00 15200.00 25.00 0.16097 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15041.01 15182.68 22.88 0.16106 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/125.ctf NX, NY, NZ: 512 512 1 MODE : real