CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/124.mrc Output diagnostic file name Micrographs_0601/124.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/124.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2224.000 8158.000 Mean, RMS : 4465.582 215.9540 TITLE 1: emi2mrc: 124.emi Sun Jan 05 16:16:21 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 15.355, Y 335.789, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.05868 10200.00 10000.00 0.00 0.06171 10400.00 10000.00 0.00 0.06449 10600.00 10000.00 0.00 0.06789 10800.00 10000.00 0.00 0.07141 11000.00 10000.00 0.00 0.07525 11200.00 10000.00 0.00 0.07930 11400.00 10000.00 0.00 0.08329 11600.00 10000.00 0.00 0.08734 11800.00 10000.00 0.00 0.09143 12000.00 10000.00 0.00 0.09595 12200.00 10000.00 0.00 0.10048 12400.00 10000.00 0.00 0.10523 12600.00 10000.00 0.00 0.10925 12800.00 10000.00 0.00 0.11145 13000.00 10000.00 0.00 0.11209 12600.00 10200.00 0.00 0.11291 12800.00 10200.00 0.00 0.11504 13000.00 10200.00 0.00 0.11559 12600.00 10400.00 0.00 0.11684 12800.00 10400.00 0.00 0.11890 13000.00 10400.00 0.00 0.11933 12600.00 10600.00 0.00 0.12088 12800.00 10600.00 0.00 0.12287 13000.00 10600.00 0.00 0.12317 12600.00 10800.00 0.00 0.12474 12800.00 10800.00 0.00 0.12670 13000.00 10800.00 0.00 0.12687 12600.00 11000.00 0.00 0.12896 12800.00 11000.00 0.00 0.13083 12600.00 11200.00 0.00 0.13288 12800.00 11200.00 0.00 0.13461 12600.00 11400.00 0.00 0.13731 12800.00 11400.00 0.00 0.13885 12600.00 11600.00 0.00 0.14173 12800.00 11600.00 0.00 0.14309 12600.00 11800.00 0.00 0.14630 12800.00 11800.00 0.00 0.14741 12400.00 12000.00 0.00 0.14743 12600.00 12000.00 0.00 0.15102 12800.00 12000.00 0.00 0.15183 12400.00 12200.00 0.00 0.15204 12600.00 12200.00 0.00 0.15552 12800.00 12200.00 0.00 0.15588 12400.00 12400.00 0.00 0.15724 12600.00 12400.00 0.00 0.16005 12400.00 12600.00 0.00 0.16081 12600.00 12600.00 0.00 0.16244 12600.00 12800.00 0.00 0.16267 12600.00 12800.00 5.00 0.16269 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12522.48 12740.46 15.94 0.16314 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/124.ctf NX, NY, NZ: 512 512 1 MODE : real