CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/123.mrc Output diagnostic file name Micrographs_0601/123.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/123.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2100.000 7656.000 Mean, RMS : 4473.444 216.5922 TITLE 1: emi2mrc: 123.emi Sun Jan 05 16:15:37 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 17.834, Y 335.437, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04852 10200.00 10000.00 0.00 0.05105 10400.00 10000.00 0.00 0.05332 10600.00 10000.00 0.00 0.05586 10800.00 10000.00 0.00 0.05835 11000.00 10000.00 0.00 0.06120 11200.00 10000.00 0.00 0.06421 11400.00 10000.00 0.00 0.06718 11600.00 10000.00 0.00 0.07051 11800.00 10000.00 0.00 0.07397 12000.00 10000.00 0.00 0.07756 12200.00 10000.00 0.00 0.08091 12400.00 10000.00 0.00 0.08448 12600.00 10000.00 0.00 0.08831 12800.00 10000.00 0.00 0.09231 13000.00 10000.00 0.00 0.09645 13200.00 10000.00 0.00 0.10102 13400.00 10000.00 0.00 0.10564 13600.00 10000.00 0.00 0.10814 13800.00 10000.00 0.00 0.10859 13600.00 10200.00 0.00 0.11083 13800.00 10200.00 0.00 0.11127 13600.00 10400.00 0.00 0.11367 13800.00 10400.00 0.00 0.11407 13400.00 10600.00 0.00 0.11409 13600.00 10600.00 0.00 0.11663 13800.00 10600.00 0.00 0.11689 13400.00 10800.00 0.00 0.11699 13600.00 10800.00 0.00 0.11948 13800.00 10800.00 0.00 0.11957 13400.00 11000.00 0.00 0.12033 13600.00 11000.00 0.00 0.12275 13400.00 11200.00 0.00 0.12372 13600.00 11200.00 0.00 0.12607 13400.00 11400.00 0.00 0.12756 13600.00 11400.00 0.00 0.12980 13400.00 11600.00 0.00 0.13130 13600.00 11600.00 0.00 0.13338 13400.00 11800.00 0.00 0.13502 13600.00 11800.00 0.00 0.13689 13400.00 12000.00 0.00 0.13873 13600.00 12000.00 0.00 0.14040 13400.00 12200.00 0.00 0.14247 13600.00 12200.00 0.00 0.14397 13400.00 12400.00 0.00 0.14648 13600.00 12400.00 0.00 0.14773 13400.00 12600.00 0.00 0.15018 13600.00 12600.00 0.00 0.15108 13400.00 12800.00 0.00 0.15436 13600.00 12800.00 0.00 0.15473 13400.00 13000.00 0.00 0.15785 13400.00 13200.00 0.00 0.16143 13400.00 13400.00 0.00 0.16439 13400.00 13600.00 5.00 0.16445 13400.00 13600.00 10.00 0.16454 13400.00 13600.00 15.00 0.16459 13400.00 13600.00 20.00 0.16459 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13369.27 13532.84 22.25 0.16486 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/123.ctf NX, NY, NZ: 512 512 1 MODE : real