CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/122.mrc Output diagnostic file name Micrographs_0601/122.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/122.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2374.000 8916.000 Mean, RMS : 4471.005 215.8028 TITLE 1: emi2mrc: 122.emi Sun Jan 05 16:14:57 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 20.363, Y 335.066, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.15462 10200.00 10000.00 0.00 0.16366 10400.00 10000.00 0.00 0.16887 10200.00 10200.00 0.00 0.17136 10400.00 10200.00 0.00 0.17522 10200.00 10400.00 0.00 0.17704 10400.00 10400.00 0.00 0.17954 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10334.31 10512.24 12.08 0.18065 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/122.ctf NX, NY, NZ: 512 512 1 MODE : real