CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/121.mrc Output diagnostic file name Micrographs_0601/121.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/121.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2140.000 8570.000 Mean, RMS : 4471.262 219.3649 TITLE 1: emi2mrc: 121.emi Sun Jan 05 16:12:53 2014 TITLE 2: kV 300, Mag 78000x, Def 18210, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 26.305, Y 337.101, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.05887 10200.00 10000.00 0.00 0.06252 10400.00 10000.00 0.00 0.06593 10600.00 10000.00 0.00 0.07002 10800.00 10000.00 0.00 0.07414 11000.00 10000.00 0.00 0.07852 11200.00 10000.00 0.00 0.08297 11400.00 10000.00 0.00 0.08749 11600.00 10000.00 0.00 0.09225 11800.00 10000.00 0.00 0.09708 12000.00 10000.00 0.00 0.10260 12200.00 10000.00 0.00 0.10800 12400.00 10000.00 0.00 0.11355 12600.00 10000.00 0.00 0.11790 12800.00 10000.00 0.00 0.11946 12600.00 10200.00 0.00 0.12185 12800.00 10200.00 0.00 0.12331 12600.00 10400.00 0.00 0.12634 12800.00 10400.00 0.00 0.12771 12600.00 10600.00 0.00 0.13107 12800.00 10600.00 0.00 0.13229 12600.00 10800.00 0.00 0.13554 12800.00 10800.00 0.00 0.13662 12600.00 11000.00 0.00 0.14058 12800.00 11000.00 0.00 0.14148 12600.00 11200.00 0.00 0.14538 12800.00 11200.00 0.00 0.14603 12400.00 11400.00 0.00 0.14630 12600.00 11400.00 0.00 0.15038 12800.00 11400.00 0.00 0.15071 12400.00 11600.00 0.00 0.15116 12600.00 11600.00 0.00 0.15516 12400.00 11800.00 0.00 0.15636 12600.00 11800.00 0.00 0.16015 12400.00 12000.00 0.00 0.16175 12600.00 12000.00 0.00 0.16526 12400.00 12200.00 0.00 0.16721 12600.00 12200.00 0.00 0.17030 12400.00 12400.00 0.00 0.17365 12600.00 12400.00 0.00 0.17545 12400.00 12600.00 0.00 0.17736 12400.00 12600.00 5.00 0.17751 12400.00 12600.00 10.00 0.17763 12400.00 12600.00 15.00 0.17772 12400.00 12600.00 20.00 0.17776 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12465.51 12668.40 18.91 0.17856 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/121.ctf NX, NY, NZ: 512 512 1 MODE : real