CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/120.mrc Output diagnostic file name Micrographs_0601/120.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/120.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2276.000 11342.00 Mean, RMS : 4469.311 219.1465 TITLE 1: emi2mrc: 120.emi Sun Jan 05 16:12:11 2014 TITLE 2: kV 300, Mag 78000x, Def 18210, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 23.854, Y 337.449, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.05762 10200.00 10000.00 0.00 0.06115 10400.00 10000.00 0.00 0.06448 10600.00 10000.00 0.00 0.06830 10800.00 10000.00 0.00 0.07223 11000.00 10000.00 0.00 0.07646 11200.00 10000.00 0.00 0.08080 11400.00 10000.00 0.00 0.08500 11600.00 10000.00 0.00 0.08901 11800.00 10000.00 0.00 0.09290 12000.00 10000.00 0.00 0.09740 12200.00 10000.00 0.00 0.10208 12400.00 10000.00 0.00 0.10720 12600.00 10000.00 0.00 0.11232 12800.00 10000.00 0.00 0.11605 13000.00 10000.00 0.00 0.11725 12800.00 10200.00 0.00 0.11962 13000.00 10200.00 0.00 0.12077 12800.00 10400.00 0.00 0.12367 13000.00 10400.00 0.00 0.12473 12800.00 10600.00 0.00 0.12800 13000.00 10600.00 0.00 0.12891 12800.00 10800.00 0.00 0.13216 13000.00 10800.00 0.00 0.13290 12800.00 11000.00 0.00 0.13662 13000.00 11000.00 0.00 0.13715 12600.00 11200.00 0.00 0.13726 12800.00 11200.00 0.00 0.14094 13000.00 11200.00 0.00 0.14121 12600.00 11400.00 0.00 0.14203 12800.00 11400.00 0.00 0.14558 13000.00 11400.00 0.00 0.14559 12600.00 11600.00 0.00 0.14673 12800.00 11600.00 0.00 0.15019 12600.00 11800.00 0.00 0.15184 12800.00 11800.00 0.00 0.15513 12600.00 12000.00 0.00 0.15685 12800.00 12000.00 0.00 0.15986 12600.00 12200.00 0.00 0.16163 12800.00 12200.00 0.00 0.16424 12600.00 12400.00 0.00 0.16678 12800.00 12400.00 0.00 0.16870 12600.00 12600.00 0.00 0.17056 12800.00 12600.00 0.00 0.17176 12600.00 12800.00 0.00 0.17327 12600.00 12800.00 5.00 0.17344 12600.00 12800.00 10.00 0.17357 12600.00 12800.00 15.00 0.17368 12600.00 12800.00 20.00 0.17374 12600.00 12800.00 25.00 0.17377 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12667.48 12831.71 22.92 0.17423 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/120.ctf NX, NY, NZ: 512 512 1 MODE : real