CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/119.mrc Output diagnostic file name Micrographs_0601/119.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/119.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2344.000 7864.000 Mean, RMS : 4471.605 218.4207 TITLE 1: emi2mrc: 119.emi Sun Jan 05 16:11:08 2014 TITLE 2: kV 300, Mag 78000x, Def 18210, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 20.892, Y 337.575, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.05390 10200.00 10000.00 0.00 0.05666 10400.00 10000.00 0.00 0.05916 10600.00 10000.00 0.00 0.06225 10800.00 10000.00 0.00 0.06529 11000.00 10000.00 0.00 0.06866 11200.00 10000.00 0.00 0.07217 11400.00 10000.00 0.00 0.07578 11600.00 10000.00 0.00 0.07963 11800.00 10000.00 0.00 0.08328 12000.00 10000.00 0.00 0.08722 12200.00 10000.00 0.00 0.09119 12400.00 10000.00 0.00 0.09557 12600.00 10000.00 0.00 0.10008 12800.00 10000.00 0.00 0.10482 13000.00 10000.00 0.00 0.10947 13200.00 10000.00 0.00 0.11205 13400.00 10000.00 0.00 0.11261 13000.00 10200.00 0.00 0.11276 13200.00 10200.00 0.00 0.11537 13400.00 10200.00 0.00 0.11587 13000.00 10400.00 0.00 0.11628 13200.00 10400.00 0.00 0.11888 13400.00 10400.00 0.00 0.11925 13000.00 10600.00 0.00 0.12004 13200.00 10600.00 0.00 0.12256 13400.00 10600.00 0.00 0.12277 13000.00 10800.00 0.00 0.12378 13200.00 10800.00 0.00 0.12620 13400.00 10800.00 0.00 0.12627 13000.00 11000.00 0.00 0.12793 13200.00 11000.00 0.00 0.13025 13000.00 11200.00 0.00 0.13193 13200.00 11200.00 0.00 0.13421 13000.00 11400.00 0.00 0.13650 13200.00 11400.00 0.00 0.13872 13000.00 11600.00 0.00 0.14115 13200.00 11600.00 0.00 0.14327 13000.00 11800.00 0.00 0.14587 13200.00 11800.00 0.00 0.14782 13000.00 12000.00 0.00 0.15049 13200.00 12000.00 0.00 0.15222 13000.00 12200.00 0.00 0.15510 13200.00 12200.00 0.00 0.15652 13000.00 12400.00 0.00 0.16018 13200.00 12400.00 0.00 0.16107 13000.00 12600.00 0.00 0.16486 13200.00 12600.00 0.00 0.16514 12800.00 12800.00 0.00 0.16585 13000.00 12800.00 0.00 0.17062 12800.00 13000.00 0.00 0.17160 13000.00 13000.00 0.00 0.17471 13000.00 13200.00 10.00 0.17478 13000.00 13200.00 15.00 0.17482 13000.00 13200.00 20.00 0.17482 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12952.54 13122.16 20.00 0.17547 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/119.ctf NX, NY, NZ: 512 512 1 MODE : real