CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/118.mrc Output diagnostic file name Micrographs_0601/118.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/118.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2276.000 8338.000 Mean, RMS : 4478.257 219.2972 TITLE 1: emi2mrc: 118.emi Sun Jan 05 16:10:22 2014 TITLE 2: kV 300, Mag 78000x, Def 18210, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 18.154, Y 337.853, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04195 10200.00 10000.00 0.00 0.04396 10400.00 10000.00 0.00 0.04573 10600.00 10000.00 0.00 0.04791 10800.00 10000.00 0.00 0.05018 11000.00 10000.00 0.00 0.05271 11200.00 10000.00 0.00 0.05521 11400.00 10000.00 0.00 0.05757 11600.00 10000.00 0.00 0.06013 11800.00 10000.00 0.00 0.06290 12000.00 10000.00 0.00 0.06606 12200.00 10000.00 0.00 0.06921 12400.00 10000.00 0.00 0.07239 12600.00 10000.00 0.00 0.07563 12800.00 10000.00 0.00 0.07904 13000.00 10000.00 0.00 0.08238 13200.00 10000.00 0.00 0.08579 13400.00 10000.00 0.00 0.08967 13600.00 10000.00 0.00 0.09348 13800.00 10000.00 0.00 0.09734 14000.00 10000.00 0.00 0.10010 14200.00 10000.00 0.00 0.10116 14400.00 10000.00 0.00 0.10142 14600.00 10000.00 0.00 0.10146 14000.00 10200.00 0.00 0.10266 14200.00 10200.00 0.00 0.10374 14400.00 10200.00 0.00 0.10400 14600.00 10200.00 0.00 0.10400 14000.00 10400.00 0.00 0.10533 14200.00 10400.00 0.00 0.10642 14400.00 10400.00 0.00 0.10662 14000.00 10600.00 0.00 0.10818 14200.00 10600.00 0.00 0.10923 14400.00 10600.00 0.00 0.10937 14000.00 10800.00 0.00 0.11118 14200.00 10800.00 0.00 0.11217 14400.00 10800.00 0.00 0.11221 14000.00 11000.00 0.00 0.11440 14200.00 11000.00 0.00 0.11530 14000.00 11200.00 0.00 0.11751 14200.00 11200.00 0.00 0.11832 14000.00 11400.00 0.00 0.12088 14200.00 11400.00 0.00 0.12159 14000.00 11600.00 0.00 0.12404 14200.00 11600.00 0.00 0.12467 13800.00 11800.00 0.00 0.12484 14000.00 11800.00 0.00 0.12748 14200.00 11800.00 0.00 0.12804 13800.00 12000.00 0.00 0.12852 14000.00 12000.00 0.00 0.13115 14200.00 12000.00 0.00 0.13165 13800.00 12200.00 0.00 0.13214 14000.00 12200.00 0.00 0.13478 14200.00 12200.00 0.00 0.13519 13800.00 12400.00 0.00 0.13619 14000.00 12400.00 0.00 0.13883 14200.00 12400.00 0.00 0.13911 13800.00 12600.00 0.00 0.14021 14000.00 12600.00 0.00 0.14280 14200.00 12600.00 0.00 0.14283 13800.00 12800.00 0.00 0.14441 14000.00 12800.00 0.00 0.14685 13800.00 13000.00 0.00 0.14842 14000.00 13000.00 0.00 0.15073 13800.00 13200.00 0.00 0.15322 14000.00 13200.00 0.00 0.15529 13800.00 13400.00 0.00 0.15802 14000.00 13400.00 0.00 0.15971 13800.00 13600.00 0.00 0.16285 14000.00 13600.00 0.00 0.16394 13800.00 13800.00 0.00 0.16813 13800.00 14000.00 0.00 0.17011 13800.00 14000.00 5.00 0.17022 13800.00 14000.00 10.00 0.17030 13800.00 14000.00 15.00 0.17033 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13817.82 14000.00 15.00 0.17036 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/118.ctf NX, NY, NZ: 512 512 1 MODE : real