CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/117.mrc Output diagnostic file name Micrographs_0601/117.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/117.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2206.000 8010.000 Mean, RMS : 4471.357 212.5975 TITLE 1: emi2mrc: 117.emi Sun Jan 05 16:09:01 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 15.648, Y 338.093, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03531 10200.00 10000.00 0.00 0.03694 10400.00 10000.00 0.00 0.03840 10600.00 10000.00 0.00 0.04029 10800.00 10000.00 0.00 0.04232 11000.00 10000.00 0.00 0.04440 11200.00 10000.00 0.00 0.04642 11400.00 10000.00 0.00 0.04853 11600.00 10000.00 0.00 0.05073 11800.00 10000.00 0.00 0.05285 12000.00 10000.00 0.00 0.05526 12200.00 10000.00 0.00 0.05784 12400.00 10000.00 0.00 0.06077 12600.00 10000.00 0.00 0.06362 12800.00 10000.00 0.00 0.06629 13000.00 10000.00 0.00 0.06909 13200.00 10000.00 0.00 0.07181 13400.00 10000.00 0.00 0.07466 13600.00 10000.00 0.00 0.07763 13800.00 10000.00 0.00 0.08066 14000.00 10000.00 0.00 0.08360 14200.00 10000.00 0.00 0.08655 14400.00 10000.00 0.00 0.08952 14600.00 10000.00 0.00 0.09219 14800.00 10000.00 0.00 0.09379 15000.00 10000.00 0.00 0.09444 15200.00 10000.00 0.00 0.09483 14800.00 10200.00 0.00 0.09560 15000.00 10200.00 0.00 0.09622 15200.00 10200.00 0.00 0.09662 14800.00 10400.00 0.00 0.09752 15000.00 10400.00 0.00 0.09819 15200.00 10400.00 0.00 0.09861 14800.00 10600.00 0.00 0.09972 15000.00 10600.00 0.00 0.10035 15200.00 10600.00 0.00 0.10067 14800.00 10800.00 0.00 0.10175 15000.00 10800.00 0.00 0.10227 15200.00 10800.00 0.00 0.10249 14800.00 11000.00 0.00 0.10387 15000.00 11000.00 0.00 0.10435 15200.00 11000.00 0.00 0.10453 14800.00 11200.00 0.00 0.10587 15000.00 11200.00 0.00 0.10632 15200.00 11200.00 0.00 0.10649 14600.00 11400.00 0.00 0.10652 14800.00 11400.00 0.00 0.10800 15000.00 11400.00 0.00 0.10843 15200.00 11400.00 0.00 0.10855 14600.00 11600.00 0.00 0.10873 14800.00 11600.00 0.00 0.11024 15000.00 11600.00 0.00 0.11062 15200.00 11600.00 0.00 0.11073 14600.00 11800.00 0.00 0.11118 14800.00 11800.00 0.00 0.11272 15000.00 11800.00 0.00 0.11307 15200.00 11800.00 0.00 0.11316 14600.00 12000.00 0.00 0.11398 14800.00 12000.00 0.00 0.11551 15000.00 12000.00 0.00 0.11580 15200.00 12000.00 0.00 0.11585 14600.00 12200.00 0.00 0.11691 14800.00 12200.00 0.00 0.11837 15000.00 12200.00 0.00 0.11858 14600.00 12400.00 0.00 0.11990 14800.00 12400.00 0.00 0.12129 15000.00 12400.00 0.00 0.12140 14600.00 12600.00 0.00 0.12279 14800.00 12600.00 0.00 0.12408 14600.00 12800.00 0.00 0.12566 14800.00 12800.00 0.00 0.12682 14600.00 13000.00 0.00 0.12818 14800.00 13000.00 0.00 0.12923 14600.00 13200.00 0.00 0.13099 14800.00 13200.00 0.00 0.13195 14600.00 13400.00 0.00 0.13389 14800.00 13400.00 0.00 0.13474 14600.00 13600.00 0.00 0.13682 14800.00 13600.00 0.00 0.13749 14600.00 13800.00 0.00 0.13963 14800.00 13800.00 0.00 0.14006 14600.00 14000.00 0.00 0.14212 14800.00 14000.00 0.00 0.14225 14400.00 14200.00 0.00 0.14263 14600.00 14200.00 0.00 0.14464 14600.00 14400.00 0.00 0.14624 14400.00 14600.00 0.00 0.14641 14600.00 14600.00 0.00 0.14781 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14567.94 14694.61 4.04 0.14809 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/117.ctf NX, NY, NZ: 512 512 1 MODE : real