CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/113.mrc Output diagnostic file name Micrographs_0601/113.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/113.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2260.000 8960.000 Mean, RMS : 4429.256 235.1161 TITLE 1: emi2mrc: 113.emi Sun Jan 05 16:06:04 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 15.421, Y 341.267, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01987 10200.00 10000.00 0.00 0.02080 10400.00 10000.00 0.00 0.02169 10600.00 10000.00 0.00 0.02275 10800.00 10000.00 0.00 0.02374 11000.00 10000.00 0.00 0.02500 11200.00 10000.00 0.00 0.02658 11400.00 10000.00 0.00 0.02824 11600.00 10000.00 0.00 0.02999 11800.00 10000.00 0.00 0.03166 12000.00 10000.00 0.00 0.03343 12200.00 10000.00 0.00 0.03524 12400.00 10000.00 0.00 0.03704 12600.00 10000.00 0.00 0.03894 12800.00 10000.00 0.00 0.04092 13000.00 10000.00 0.00 0.04293 13200.00 10000.00 0.00 0.04507 13400.00 10000.00 0.00 0.04732 13600.00 10000.00 0.00 0.04962 13800.00 10000.00 0.00 0.05214 14000.00 10000.00 0.00 0.05472 14200.00 10000.00 0.00 0.05734 14400.00 10000.00 0.00 0.05985 14600.00 10000.00 0.00 0.06252 14800.00 10000.00 0.00 0.06545 15000.00 10000.00 0.00 0.06848 15200.00 10000.00 0.00 0.07187 15400.00 10000.00 0.00 0.07522 15600.00 10000.00 0.00 0.07866 15800.00 10000.00 0.00 0.08201 16000.00 10000.00 0.00 0.08422 16200.00 10000.00 0.00 0.08505 16000.00 10200.00 0.00 0.08589 16200.00 10200.00 0.00 0.08670 16000.00 10400.00 0.00 0.08761 16200.00 10400.00 0.00 0.08834 16000.00 10600.00 0.00 0.08923 16200.00 10600.00 0.00 0.08988 16000.00 10800.00 0.00 0.09096 16200.00 10800.00 0.00 0.09155 16000.00 11000.00 0.00 0.09296 16200.00 11000.00 0.00 0.09350 16000.00 11200.00 0.00 0.09479 16200.00 11200.00 0.00 0.09530 16000.00 11400.00 0.00 0.09693 16200.00 11400.00 0.00 0.09739 16000.00 11600.00 0.00 0.09899 16200.00 11600.00 0.00 0.09941 16000.00 11800.00 0.00 0.10111 16200.00 11800.00 0.00 0.10154 16000.00 12000.00 0.00 0.10351 16200.00 12000.00 0.00 0.10394 15800.00 12200.00 0.00 0.10405 16000.00 12200.00 0.00 0.10607 16200.00 12200.00 0.00 0.10646 15800.00 12400.00 0.00 0.10675 16000.00 12400.00 0.00 0.10873 16200.00 12400.00 0.00 0.10905 15800.00 12600.00 0.00 0.10921 16000.00 12600.00 0.00 0.11116 16200.00 12600.00 0.00 0.11142 15800.00 12800.00 0.00 0.11192 16000.00 12800.00 0.00 0.11385 16200.00 12800.00 0.00 0.11407 15800.00 13000.00 0.00 0.11463 16000.00 13000.00 0.00 0.11658 16200.00 13000.00 0.00 0.11676 15800.00 13200.00 0.00 0.11754 16000.00 13200.00 0.00 0.11951 16200.00 13200.00 0.00 0.11961 15800.00 13400.00 0.00 0.12058 16000.00 13400.00 0.00 0.12251 15800.00 13600.00 0.00 0.12374 16000.00 13600.00 0.00 0.12562 15800.00 13800.00 0.00 0.12693 16000.00 13800.00 0.00 0.12877 15800.00 14000.00 0.00 0.13007 16000.00 14000.00 0.00 0.13186 15800.00 14200.00 0.00 0.13352 16000.00 14200.00 0.00 0.13519 15800.00 14400.00 0.00 0.13671 16000.00 14400.00 0.00 0.13818 15800.00 14600.00 0.00 0.14026 16000.00 14600.00 0.00 0.14147 15800.00 14800.00 0.00 0.14359 16000.00 14800.00 0.00 0.14450 15800.00 15000.00 0.00 0.14688 16000.00 15000.00 0.00 0.14741 15800.00 15200.00 0.00 0.15010 16000.00 15200.00 0.00 0.15024 15800.00 15400.00 0.00 0.15271 15600.00 15600.00 0.00 0.15289 15800.00 15600.00 0.00 0.15500 15800.00 15800.00 0.00 0.15594 15800.00 16000.00 0.00 0.15656 15800.00 16000.00 5.00 0.15666 15800.00 16000.00 10.00 0.15672 15800.00 16000.00 15.00 0.15674 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15755.14 16025.88 17.49 0.15682 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/113.ctf NX, NY, NZ: 512 512 1 MODE : real