CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/112.mrc Output diagnostic file name Micrographs_0601/112.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/112.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2296.000 8058.000 Mean, RMS : 4480.223 219.5475 TITLE 1: emi2mrc: 112.emi Sun Jan 05 15:15:19 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 20.982, Y 340.353, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02401 10200.00 10000.00 0.00 0.02525 10400.00 10000.00 0.00 0.02623 10600.00 10000.00 0.00 0.02747 10800.00 10000.00 0.00 0.02868 11000.00 10000.00 0.00 0.03001 11200.00 10000.00 0.00 0.03152 11400.00 10000.00 0.00 0.03296 11600.00 10000.00 0.00 0.03468 11800.00 10000.00 0.00 0.03663 12000.00 10000.00 0.00 0.03896 12200.00 10000.00 0.00 0.04140 12400.00 10000.00 0.00 0.04397 12600.00 10000.00 0.00 0.04670 12800.00 10000.00 0.00 0.04944 13000.00 10000.00 0.00 0.05238 13200.00 10000.00 0.00 0.05540 13400.00 10000.00 0.00 0.05844 13600.00 10000.00 0.00 0.06140 13800.00 10000.00 0.00 0.06442 14000.00 10000.00 0.00 0.06761 14200.00 10000.00 0.00 0.07105 14400.00 10000.00 0.00 0.07466 14600.00 10000.00 0.00 0.07840 14800.00 10000.00 0.00 0.08252 15000.00 10000.00 0.00 0.08626 15200.00 10000.00 0.00 0.08829 15400.00 10000.00 0.00 0.08881 15600.00 10000.00 0.00 0.08901 15200.00 10200.00 0.00 0.09048 15400.00 10200.00 0.00 0.09100 15600.00 10200.00 0.00 0.09115 15200.00 10400.00 0.00 0.09258 15400.00 10400.00 0.00 0.09311 15600.00 10400.00 0.00 0.09319 15200.00 10600.00 0.00 0.09475 15400.00 10600.00 0.00 0.09528 15600.00 10600.00 0.00 0.09529 15200.00 10800.00 0.00 0.09713 15400.00 10800.00 0.00 0.09760 15000.00 11000.00 0.00 0.09763 15200.00 11000.00 0.00 0.09972 15400.00 11000.00 0.00 0.10010 15000.00 11200.00 0.00 0.10018 15200.00 11200.00 0.00 0.10228 15400.00 11200.00 0.00 0.10258 15000.00 11400.00 0.00 0.10303 15200.00 11400.00 0.00 0.10509 15400.00 11400.00 0.00 0.10529 15000.00 11600.00 0.00 0.10576 15200.00 11600.00 0.00 0.10775 15400.00 11600.00 0.00 0.10781 15000.00 11800.00 0.00 0.10855 15200.00 11800.00 0.00 0.11048 15000.00 12000.00 0.00 0.11145 15200.00 12000.00 0.00 0.11332 15000.00 12200.00 0.00 0.11445 15200.00 12200.00 0.00 0.11624 15000.00 12400.00 0.00 0.11762 15200.00 12400.00 0.00 0.11936 15000.00 12600.00 0.00 0.12069 15200.00 12600.00 0.00 0.12241 15000.00 12800.00 0.00 0.12413 15200.00 12800.00 0.00 0.12582 15000.00 13000.00 0.00 0.12767 15200.00 13000.00 0.00 0.12930 15000.00 13200.00 0.00 0.13150 15200.00 13200.00 0.00 0.13307 15000.00 13400.00 0.00 0.13531 15200.00 13400.00 0.00 0.13680 15000.00 13600.00 0.00 0.13934 15200.00 13600.00 0.00 0.14072 15000.00 13800.00 0.00 0.14374 15200.00 13800.00 0.00 0.14496 15000.00 14000.00 0.00 0.14812 15200.00 14000.00 0.00 0.14912 15000.00 14200.00 0.00 0.15297 15200.00 14200.00 0.00 0.15360 14800.00 14400.00 0.00 0.15379 15000.00 14400.00 0.00 0.15754 15200.00 14400.00 0.00 0.15768 14800.00 14600.00 0.00 0.15915 15000.00 14600.00 0.00 0.16242 14800.00 14800.00 0.00 0.16440 15000.00 14800.00 0.00 0.16689 14800.00 15000.00 0.00 0.16834 15000.00 15000.00 0.00 0.17004 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15112.08 14921.03 -65.74 0.17089 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/112.ctf NX, NY, NZ: 512 512 1 MODE : real