CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/110.mrc Output diagnostic file name Micrographs_0601/110.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/110.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2028.000 7656.000 Mean, RMS : 4476.728 224.7641 TITLE 1: emi2mrc: 110.emi Sun Jan 05 15:13:02 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 26.300, Y 339.611, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01589 10200.00 10000.00 0.00 0.01677 10400.00 10000.00 0.00 0.01755 10600.00 10000.00 0.00 0.01850 10800.00 10000.00 0.00 0.01944 11000.00 10000.00 0.00 0.02045 11200.00 10000.00 0.00 0.02154 11400.00 10000.00 0.00 0.02276 11600.00 10000.00 0.00 0.02415 11800.00 10000.00 0.00 0.02563 12000.00 10000.00 0.00 0.02730 12200.00 10000.00 0.00 0.02908 12400.00 10000.00 0.00 0.03101 12600.00 10000.00 0.00 0.03283 12800.00 10000.00 0.00 0.03489 13000.00 10000.00 0.00 0.03717 13200.00 10000.00 0.00 0.03948 13400.00 10000.00 0.00 0.04208 13600.00 10000.00 0.00 0.04464 13800.00 10000.00 0.00 0.04735 14000.00 10000.00 0.00 0.05028 14200.00 10000.00 0.00 0.05329 14400.00 10000.00 0.00 0.05610 14600.00 10000.00 0.00 0.05883 14800.00 10000.00 0.00 0.06176 15000.00 10000.00 0.00 0.06470 15200.00 10000.00 0.00 0.06793 15400.00 10000.00 0.00 0.07111 15600.00 10000.00 0.00 0.07437 15800.00 10000.00 0.00 0.07776 16000.00 10000.00 0.00 0.08051 16200.00 10000.00 0.00 0.08220 16400.00 10000.00 0.00 0.08294 16600.00 10000.00 0.00 0.08312 16200.00 10200.00 0.00 0.08378 16400.00 10200.00 0.00 0.08453 16600.00 10200.00 0.00 0.08466 16200.00 10400.00 0.00 0.08547 16400.00 10400.00 0.00 0.08617 16600.00 10400.00 0.00 0.08623 16200.00 10600.00 0.00 0.08727 16400.00 10600.00 0.00 0.08791 16600.00 10600.00 0.00 0.08794 16200.00 10800.00 0.00 0.08912 16400.00 10800.00 0.00 0.08971 16200.00 11000.00 0.00 0.09107 16400.00 11000.00 0.00 0.09160 16200.00 11200.00 0.00 0.09284 16400.00 11200.00 0.00 0.09332 16200.00 11400.00 0.00 0.09492 16400.00 11400.00 0.00 0.09535 16000.00 11600.00 0.00 0.09537 16200.00 11600.00 0.00 0.09713 16400.00 11600.00 0.00 0.09746 16000.00 11800.00 0.00 0.09780 16200.00 11800.00 0.00 0.09947 16400.00 11800.00 0.00 0.09970 16000.00 12000.00 0.00 0.10043 16200.00 12000.00 0.00 0.10204 16400.00 12000.00 0.00 0.10220 16000.00 12200.00 0.00 0.10318 16200.00 12200.00 0.00 0.10472 16400.00 12200.00 0.00 0.10484 16000.00 12400.00 0.00 0.10599 16200.00 12400.00 0.00 0.10748 16400.00 12400.00 0.00 0.10755 16000.00 12600.00 0.00 0.10869 16200.00 12600.00 0.00 0.11016 16400.00 12600.00 0.00 0.11018 16000.00 12800.00 0.00 0.11160 16200.00 12800.00 0.00 0.11304 16000.00 13000.00 0.00 0.11447 16200.00 13000.00 0.00 0.11586 16000.00 13200.00 0.00 0.11762 16200.00 13200.00 0.00 0.11896 16000.00 13400.00 0.00 0.12091 16200.00 13400.00 0.00 0.12219 16000.00 13600.00 0.00 0.12436 16200.00 13600.00 0.00 0.12561 16000.00 13800.00 0.00 0.12797 16200.00 13800.00 0.00 0.12917 16000.00 14000.00 0.00 0.13138 16200.00 14000.00 0.00 0.13250 16000.00 14200.00 0.00 0.13500 16200.00 14200.00 0.00 0.13602 16000.00 14400.00 0.00 0.13851 16200.00 14400.00 0.00 0.13940 15800.00 14600.00 0.00 0.13966 16000.00 14600.00 0.00 0.14228 16200.00 14600.00 0.00 0.14304 15800.00 14800.00 0.00 0.14344 16000.00 14800.00 0.00 0.14604 16200.00 14800.00 0.00 0.14667 15800.00 15000.00 0.00 0.14757 16000.00 15000.00 0.00 0.15013 16200.00 15000.00 0.00 0.15049 15800.00 15200.00 0.00 0.15179 16000.00 15200.00 0.00 0.15416 15800.00 15400.00 0.00 0.15570 16000.00 15400.00 0.00 0.15780 15800.00 15600.00 0.00 0.15985 16000.00 15600.00 0.00 0.16154 15800.00 15800.00 0.00 0.16305 16000.00 15800.00 0.00 0.16442 15800.00 16000.00 0.00 0.16547 16000.00 16000.00 0.00 0.16608 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15910.41 16044.51 23.92 0.16638 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/110.ctf NX, NY, NZ: 512 512 1 MODE : real