CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/104.mrc Output diagnostic file name Micrographs_0601/104.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/104.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2200.000 7852.000 Mean, RMS : 4487.953 224.0072 TITLE 1: emi2mrc: 104.emi Sun Jan 05 15:08:06 2014 TITLE 2: kV 300, Mag 78000x, Def 20350, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 13.836, Y 343.769, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00266 10200.00 10000.00 0.00 0.00278 10600.00 10000.00 0.00 0.00283 10800.00 10000.00 0.00 0.00299 11000.00 10000.00 0.00 0.00311 11200.00 10000.00 0.00 0.00326 11400.00 10000.00 0.00 0.00341 11600.00 10000.00 0.00 0.00370 11800.00 10000.00 0.00 0.00396 12000.00 10000.00 0.00 0.00420 12200.00 10000.00 0.00 0.00457 12400.00 10000.00 0.00 0.00496 12600.00 10000.00 0.00 0.00544 12800.00 10000.00 0.00 0.00588 13000.00 10000.00 0.00 0.00637 13200.00 10000.00 0.00 0.00693 13400.00 10000.00 0.00 0.00758 13600.00 10000.00 0.00 0.00839 13800.00 10000.00 0.00 0.00920 14000.00 10000.00 0.00 0.01021 14200.00 10000.00 0.00 0.01131 14400.00 10000.00 0.00 0.01224 14600.00 10000.00 0.00 0.01315 14800.00 10000.00 0.00 0.01419 15000.00 10000.00 0.00 0.01539 15200.00 10000.00 0.00 0.01657 15400.00 10000.00 0.00 0.01770 15600.00 10000.00 0.00 0.01898 15800.00 10000.00 0.00 0.02036 16000.00 10000.00 0.00 0.02173 16200.00 10000.00 0.00 0.02313 16400.00 10000.00 0.00 0.02455 16600.00 10000.00 0.00 0.02592 16800.00 10000.00 0.00 0.02736 17000.00 10000.00 0.00 0.02884 17200.00 10000.00 0.00 0.03030 17400.00 10000.00 0.00 0.03189 17600.00 10000.00 0.00 0.03358 17800.00 10000.00 0.00 0.03542 18000.00 10000.00 0.00 0.03728 18200.00 10000.00 0.00 0.03912 18400.00 10000.00 0.00 0.04108 18600.00 10000.00 0.00 0.04307 18800.00 10000.00 0.00 0.04516 19000.00 10000.00 0.00 0.04739 19200.00 10000.00 0.00 0.04979 19400.00 10000.00 0.00 0.05238 19600.00 10000.00 0.00 0.05494 19800.00 10000.00 0.00 0.05692 20000.00 10000.00 0.00 0.05796 20200.00 10000.00 0.00 0.05851 20400.00 10000.00 0.00 0.05893 20600.00 10000.00 0.00 0.05919 20200.00 10200.00 0.00 0.05927 20400.00 10200.00 0.00 0.05967 20600.00 10200.00 0.00 0.05992 20200.00 10400.00 0.00 0.06007 20400.00 10400.00 0.00 0.06047 20600.00 10400.00 0.00 0.06070 20200.00 10600.00 0.00 0.06085 20400.00 10600.00 0.00 0.06123 20600.00 10600.00 0.00 0.06144 20200.00 10800.00 0.00 0.06167 20400.00 10800.00 0.00 0.06203 20600.00 10800.00 0.00 0.06219 20200.00 11000.00 0.00 0.06255 20400.00 11000.00 0.00 0.06287 20600.00 11000.00 0.00 0.06299 20200.00 11200.00 0.00 0.06335 20400.00 11200.00 0.00 0.06364 20600.00 11200.00 0.00 0.06374 20000.00 11400.00 0.00 0.06389 20200.00 11400.00 0.00 0.06429 20400.00 11400.00 0.00 0.06457 20600.00 11400.00 0.00 0.06467 20000.00 11600.00 0.00 0.06480 20200.00 11600.00 0.00 0.06519 20400.00 11600.00 0.00 0.06547 20600.00 11600.00 0.00 0.06555 20000.00 11800.00 0.00 0.06577 20200.00 11800.00 0.00 0.06614 20400.00 11800.00 0.00 0.06642 20600.00 11800.00 0.00 0.06647 20000.00 12000.00 0.00 0.06679 20200.00 12000.00 0.00 0.06716 20400.00 12000.00 0.00 0.06741 20600.00 12000.00 0.00 0.06743 20000.00 12200.00 0.00 0.06780 20200.00 12200.00 0.00 0.06815 20400.00 12200.00 0.00 0.06840 20600.00 12200.00 0.00 0.06841 20000.00 12400.00 0.00 0.06883 20200.00 12400.00 0.00 0.06921 20400.00 12400.00 0.00 0.06948 20600.00 12400.00 0.00 0.06953 20000.00 12600.00 0.00 0.06986 20200.00 12600.00 0.00 0.07027 20400.00 12600.00 0.00 0.07055 20600.00 12600.00 0.00 0.07061 20000.00 12800.00 0.00 0.07101 20200.00 12800.00 0.00 0.07143 20400.00 12800.00 0.00 0.07169 20600.00 12800.00 0.00 0.07172 20000.00 13000.00 0.00 0.07211 20200.00 13000.00 0.00 0.07254 20400.00 13000.00 0.00 0.07280 20600.00 13000.00 0.00 0.07283 20000.00 13200.00 0.00 0.07342 20200.00 13200.00 0.00 0.07386 20400.00 13200.00 0.00 0.07412 20600.00 13200.00 0.00 0.07418 20000.00 13400.00 0.00 0.07483 20200.00 13400.00 0.00 0.07530 20400.00 13400.00 0.00 0.07557 20600.00 13400.00 0.00 0.07563 20000.00 13600.00 0.00 0.07633 20200.00 13600.00 0.00 0.07683 20400.00 13600.00 0.00 0.07711 20600.00 13600.00 0.00 0.07715 20000.00 13800.00 0.00 0.07797 20200.00 13800.00 0.00 0.07848 20400.00 13800.00 0.00 0.07872 20600.00 13800.00 0.00 0.07873 20000.00 14000.00 0.00 0.07954 20200.00 14000.00 0.00 0.08005 20400.00 14000.00 0.00 0.08027 20600.00 14000.00 0.00 0.08027 20000.00 14200.00 0.00 0.08117 20200.00 14200.00 0.00 0.08170 20400.00 14200.00 0.00 0.08188 20000.00 14400.00 0.00 0.08279 20200.00 14400.00 0.00 0.08327 20400.00 14400.00 0.00 0.08338 19800.00 14600.00 0.00 0.08349 20000.00 14600.00 0.00 0.08447 20200.00 14600.00 0.00 0.08489 20400.00 14600.00 0.00 0.08496 19800.00 14800.00 0.00 0.08518 20000.00 14800.00 0.00 0.08617 20200.00 14800.00 0.00 0.08657 20400.00 14800.00 0.00 0.08666 19800.00 15000.00 0.00 0.08705 20000.00 15000.00 0.00 0.08807 20200.00 15000.00 0.00 0.08849 20400.00 15000.00 0.00 0.08858 19800.00 15200.00 0.00 0.08904 20000.00 15200.00 0.00 0.09011 20200.00 15200.00 0.00 0.09051 20400.00 15200.00 0.00 0.09056 19800.00 15400.00 0.00 0.09117 20000.00 15400.00 0.00 0.09224 20200.00 15400.00 0.00 0.09259 20400.00 15400.00 0.00 0.09259 19800.00 15600.00 0.00 0.09358 20000.00 15600.00 0.00 0.09458 20200.00 15600.00 0.00 0.09482 19800.00 15800.00 0.00 0.09587 20000.00 15800.00 0.00 0.09674 20200.00 15800.00 0.00 0.09689 19800.00 16000.00 0.00 0.09811 20000.00 16000.00 0.00 0.09891 20200.00 16000.00 0.00 0.09905 19800.00 16200.00 0.00 0.10035 20000.00 16200.00 0.00 0.10116 20200.00 16200.00 0.00 0.10130 19800.00 16400.00 0.00 0.10283 20000.00 16400.00 0.00 0.10366 20200.00 16400.00 0.00 0.10373 19800.00 16600.00 0.00 0.10539 20000.00 16600.00 0.00 0.10616 19800.00 16800.00 0.00 0.10793 20000.00 16800.00 0.00 0.10862 19800.00 17000.00 0.00 0.11046 20000.00 17000.00 0.00 0.11108 19800.00 17200.00 0.00 0.11301 20000.00 17200.00 0.00 0.11352 19800.00 17400.00 0.00 0.11566 20000.00 17400.00 0.00 0.11604 19800.00 17600.00 0.00 0.11819 20000.00 17600.00 0.00 0.11850 19600.00 17800.00 0.00 0.11899 19800.00 17800.00 0.00 0.12117 20000.00 17800.00 0.00 0.12140 19600.00 18000.00 0.00 0.12207 19800.00 18000.00 0.00 0.12420 20000.00 18000.00 0.00 0.12426 19600.00 18200.00 0.00 0.12520 19800.00 18200.00 0.00 0.12726 19600.00 18400.00 0.00 0.12840 19800.00 18400.00 0.00 0.13039 19600.00 18600.00 0.00 0.13165 19800.00 18600.00 0.00 0.13353 19600.00 18800.00 0.00 0.13512 19800.00 18800.00 0.00 0.13683 19600.00 19000.00 0.00 0.13841 19800.00 19000.00 0.00 0.13989 19600.00 19200.00 0.00 0.14204 19800.00 19200.00 0.00 0.14319 19600.00 19400.00 0.00 0.14528 19800.00 19400.00 0.00 0.14605 19600.00 19600.00 0.00 0.14852 19800.00 19600.00 0.00 0.14852 19600.00 19800.00 0.00 0.14974 19600.00 19800.00 5.00 0.14989 19600.00 19800.00 10.00 0.15002 19600.00 19800.00 15.00 0.15012 19600.00 19800.00 20.00 0.15019 19600.00 19800.00 25.00 0.15022 19600.00 19800.00 30.00 0.15022 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19620.74 19774.76 27.38 0.15026 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/104.ctf NX, NY, NZ: 512 512 1 MODE : real