CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/100.mrc Output diagnostic file name Micrographs_0601/100.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/100.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2382.000 8336.000 Mean, RMS : 4454.466 214.8102 TITLE 1: emi2mrc: 100.emi Sun Jan 05 15:03:53 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.10%, Obj 88.08%, Dif 73.67% TITLE 3: X 24.071, Y 345.032, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02872 10200.00 10000.00 0.00 0.03051 10400.00 10000.00 0.00 0.03197 10600.00 10000.00 0.00 0.03358 10800.00 10000.00 0.00 0.03537 11000.00 10000.00 0.00 0.03759 11200.00 10000.00 0.00 0.03993 11400.00 10000.00 0.00 0.04217 11600.00 10000.00 0.00 0.04448 11800.00 10000.00 0.00 0.04686 12000.00 10000.00 0.00 0.04961 12200.00 10000.00 0.00 0.05242 12400.00 10000.00 0.00 0.05539 12600.00 10000.00 0.00 0.05826 12800.00 10000.00 0.00 0.06106 13000.00 10000.00 0.00 0.06413 13200.00 10000.00 0.00 0.06731 13400.00 10000.00 0.00 0.07043 13600.00 10000.00 0.00 0.07320 13800.00 10000.00 0.00 0.07606 14000.00 10000.00 0.00 0.07895 14200.00 10000.00 0.00 0.08174 14400.00 10000.00 0.00 0.08420 14600.00 10000.00 0.00 0.08625 14800.00 10000.00 0.00 0.08780 15000.00 10000.00 0.00 0.08848 15200.00 10000.00 0.00 0.08879 14800.00 10200.00 0.00 0.08970 15000.00 10200.00 0.00 0.09037 15200.00 10200.00 0.00 0.09063 14800.00 10400.00 0.00 0.09191 15000.00 10400.00 0.00 0.09251 15200.00 10400.00 0.00 0.09270 14600.00 10600.00 0.00 0.09278 14800.00 10600.00 0.00 0.09430 15000.00 10600.00 0.00 0.09478 15200.00 10600.00 0.00 0.09490 14600.00 10800.00 0.00 0.09512 14800.00 10800.00 0.00 0.09655 15000.00 10800.00 0.00 0.09695 15200.00 10800.00 0.00 0.09708 14600.00 11000.00 0.00 0.09756 14800.00 11000.00 0.00 0.09894 15000.00 11000.00 0.00 0.09933 15200.00 11000.00 0.00 0.09947 14600.00 11200.00 0.00 0.09995 14800.00 11200.00 0.00 0.10129 15000.00 11200.00 0.00 0.10164 15200.00 11200.00 0.00 0.10175 14600.00 11400.00 0.00 0.10251 14800.00 11400.00 0.00 0.10379 15000.00 11400.00 0.00 0.10410 15200.00 11400.00 0.00 0.10414 14600.00 11600.00 0.00 0.10506 14800.00 11600.00 0.00 0.10628 15000.00 11600.00 0.00 0.10652 14600.00 11800.00 0.00 0.10776 14800.00 11800.00 0.00 0.10888 15000.00 11800.00 0.00 0.10906 14600.00 12000.00 0.00 0.11048 14800.00 12000.00 0.00 0.11153 15000.00 12000.00 0.00 0.11165 14600.00 12200.00 0.00 0.11320 14800.00 12200.00 0.00 0.11419 15000.00 12200.00 0.00 0.11423 14600.00 12400.00 0.00 0.11602 14800.00 12400.00 0.00 0.11695 14600.00 12600.00 0.00 0.11868 14800.00 12600.00 0.00 0.11958 14400.00 12800.00 0.00 0.12002 14600.00 12800.00 0.00 0.12175 14800.00 12800.00 0.00 0.12255 14400.00 13000.00 0.00 0.12299 14600.00 13000.00 0.00 0.12462 14800.00 13000.00 0.00 0.12526 14400.00 13200.00 0.00 0.12590 14600.00 13200.00 0.00 0.12739 14800.00 13200.00 0.00 0.12789 14400.00 13400.00 0.00 0.12876 14600.00 13400.00 0.00 0.13014 14800.00 13400.00 0.00 0.13053 14400.00 13600.00 0.00 0.13177 14600.00 13600.00 0.00 0.13300 14800.00 13600.00 0.00 0.13322 14400.00 13800.00 0.00 0.13475 14600.00 13800.00 0.00 0.13578 14800.00 13800.00 0.00 0.13582 14400.00 14000.00 0.00 0.13741 14600.00 14000.00 0.00 0.13825 14200.00 14200.00 0.00 0.13878 14400.00 14200.00 0.00 0.14035 14600.00 14200.00 0.00 0.14081 14200.00 14400.00 0.00 0.14087 14400.00 14400.00 0.00 0.14166 14600.00 14400.00 0.00 0.14193 14400.00 14600.00 0.00 0.14219 14600.00 14600.00 0.00 0.14243 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14504.31 14693.89 36.02 0.14258 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/100.ctf NX, NY, NZ: 512 512 1 MODE : real