CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/099.mrc Output diagnostic file name Micrographs_0601/099.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/099.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2298.000 7778.000 Mean, RMS : 4457.305 219.1858 TITLE 1: emi2mrc: 099.emi Sun Jan 05 15:03:06 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.10%, Obj 88.08%, Dif 73.67% TITLE 3: X 26.785, Y 344.648, Z -97.511, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03132 10200.00 10000.00 0.00 0.03308 10400.00 10000.00 0.00 0.03481 10600.00 10000.00 0.00 0.03670 10800.00 10000.00 0.00 0.03852 11000.00 10000.00 0.00 0.04061 11200.00 10000.00 0.00 0.04291 11400.00 10000.00 0.00 0.04525 11600.00 10000.00 0.00 0.04780 11800.00 10000.00 0.00 0.05043 12000.00 10000.00 0.00 0.05319 12200.00 10000.00 0.00 0.05609 12400.00 10000.00 0.00 0.05919 12600.00 10000.00 0.00 0.06214 12800.00 10000.00 0.00 0.06525 13000.00 10000.00 0.00 0.06847 13200.00 10000.00 0.00 0.07157 13400.00 10000.00 0.00 0.07492 13600.00 10000.00 0.00 0.07820 13800.00 10000.00 0.00 0.08144 14000.00 10000.00 0.00 0.08460 14200.00 10000.00 0.00 0.08778 14400.00 10000.00 0.00 0.09071 14600.00 10000.00 0.00 0.09308 14800.00 10000.00 0.00 0.09486 15000.00 10000.00 0.00 0.09588 15200.00 10000.00 0.00 0.09614 14800.00 10200.00 0.00 0.09684 15000.00 10200.00 0.00 0.09780 15200.00 10200.00 0.00 0.09802 14800.00 10400.00 0.00 0.09883 15000.00 10400.00 0.00 0.09975 15200.00 10400.00 0.00 0.09997 14800.00 10600.00 0.00 0.10096 15000.00 10600.00 0.00 0.10185 15200.00 10600.00 0.00 0.10205 14800.00 10800.00 0.00 0.10307 15000.00 10800.00 0.00 0.10394 15200.00 10800.00 0.00 0.10413 14800.00 11000.00 0.00 0.10544 15000.00 11000.00 0.00 0.10625 15200.00 11000.00 0.00 0.10641 14800.00 11200.00 0.00 0.10776 15000.00 11200.00 0.00 0.10849 15200.00 11200.00 0.00 0.10861 14800.00 11400.00 0.00 0.11022 15000.00 11400.00 0.00 0.11091 15200.00 11400.00 0.00 0.11102 14600.00 11600.00 0.00 0.11106 14800.00 11600.00 0.00 0.11270 15000.00 11600.00 0.00 0.11335 15200.00 11600.00 0.00 0.11345 14600.00 11800.00 0.00 0.11380 14800.00 11800.00 0.00 0.11541 15000.00 11800.00 0.00 0.11602 15200.00 11800.00 0.00 0.11607 14600.00 12000.00 0.00 0.11672 14800.00 12000.00 0.00 0.11828 15000.00 12000.00 0.00 0.11884 14600.00 12200.00 0.00 0.11962 14800.00 12200.00 0.00 0.12112 15000.00 12200.00 0.00 0.12164 14600.00 12400.00 0.00 0.12260 14800.00 12400.00 0.00 0.12407 15000.00 12400.00 0.00 0.12454 14600.00 12600.00 0.00 0.12556 14800.00 12600.00 0.00 0.12699 15000.00 12600.00 0.00 0.12740 14600.00 12800.00 0.00 0.12873 14800.00 12800.00 0.00 0.13011 15000.00 12800.00 0.00 0.13038 14600.00 13000.00 0.00 0.13167 14800.00 13000.00 0.00 0.13299 15000.00 13000.00 0.00 0.13312 14600.00 13200.00 0.00 0.13481 14800.00 13200.00 0.00 0.13605 14600.00 13400.00 0.00 0.13778 14800.00 13400.00 0.00 0.13890 14600.00 13600.00 0.00 0.14081 14800.00 13600.00 0.00 0.14173 14400.00 13800.00 0.00 0.14191 14600.00 13800.00 0.00 0.14373 14800.00 13800.00 0.00 0.14434 14400.00 14000.00 0.00 0.14439 14600.00 14000.00 0.00 0.14591 14800.00 14000.00 0.00 0.14617 14400.00 14200.00 0.00 0.14704 14600.00 14200.00 0.00 0.14812 14400.00 14400.00 0.00 0.14847 14600.00 14400.00 0.00 0.14932 14400.00 14600.00 0.00 0.14962 14600.00 14600.00 0.00 0.15032 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14568.88 14718.82 2.21 0.15057 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/099.ctf NX, NY, NZ: 512 512 1 MODE : real