CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/094.mrc Output diagnostic file name Micrographs_0601/094.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/094.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2270.000 7646.000 Mean, RMS : 4358.703 211.0744 TITLE 1: emi2mrc: 094.emi Sun Jan 05 14:55:49 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 83.387, Y 311.502, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02916 10200.00 10000.00 0.00 0.03058 10400.00 10000.00 0.00 0.03179 10600.00 10000.00 0.00 0.03316 10800.00 10000.00 0.00 0.03461 11000.00 10000.00 0.00 0.03619 11200.00 10000.00 0.00 0.03768 11400.00 10000.00 0.00 0.03927 11600.00 10000.00 0.00 0.04109 11800.00 10000.00 0.00 0.04286 12000.00 10000.00 0.00 0.04490 12200.00 10000.00 0.00 0.04717 12400.00 10000.00 0.00 0.04948 12600.00 10000.00 0.00 0.05161 12800.00 10000.00 0.00 0.05375 13000.00 10000.00 0.00 0.05594 13200.00 10000.00 0.00 0.05817 13400.00 10000.00 0.00 0.06048 13600.00 10000.00 0.00 0.06275 13800.00 10000.00 0.00 0.06506 14000.00 10000.00 0.00 0.06723 14200.00 10000.00 0.00 0.06954 14400.00 10000.00 0.00 0.07197 14600.00 10000.00 0.00 0.07447 14800.00 10000.00 0.00 0.07702 15000.00 10000.00 0.00 0.07955 15200.00 10000.00 0.00 0.08230 15400.00 10000.00 0.00 0.08485 15600.00 10000.00 0.00 0.08668 15800.00 10000.00 0.00 0.08772 16000.00 10000.00 0.00 0.08832 16200.00 10000.00 0.00 0.08839 15800.00 10200.00 0.00 0.08918 16000.00 10200.00 0.00 0.08979 16200.00 10200.00 0.00 0.08985 15800.00 10400.00 0.00 0.09089 16000.00 10400.00 0.00 0.09147 16200.00 10400.00 0.00 0.09149 15600.00 10600.00 0.00 0.09164 15800.00 10600.00 0.00 0.09266 16000.00 10600.00 0.00 0.09316 15600.00 10800.00 0.00 0.09333 15800.00 10800.00 0.00 0.09426 16000.00 10800.00 0.00 0.09468 15600.00 11000.00 0.00 0.09520 15800.00 11000.00 0.00 0.09607 16000.00 11000.00 0.00 0.09645 15600.00 11200.00 0.00 0.09696 15800.00 11200.00 0.00 0.09781 16000.00 11200.00 0.00 0.09816 15600.00 11400.00 0.00 0.09904 15800.00 11400.00 0.00 0.09985 16000.00 11400.00 0.00 0.10018 15600.00 11600.00 0.00 0.10111 15800.00 11600.00 0.00 0.10186 16000.00 11600.00 0.00 0.10216 15600.00 11800.00 0.00 0.10312 15800.00 11800.00 0.00 0.10386 16000.00 11800.00 0.00 0.10418 15600.00 12000.00 0.00 0.10524 15800.00 12000.00 0.00 0.10602 16000.00 12000.00 0.00 0.10632 15600.00 12200.00 0.00 0.10748 15800.00 12200.00 0.00 0.10822 16000.00 12200.00 0.00 0.10844 15600.00 12400.00 0.00 0.10994 15800.00 12400.00 0.00 0.11064 16000.00 12400.00 0.00 0.11077 15400.00 12600.00 0.00 0.11081 15600.00 12600.00 0.00 0.11244 15800.00 12600.00 0.00 0.11310 16000.00 12600.00 0.00 0.11315 15400.00 12800.00 0.00 0.11350 15600.00 12800.00 0.00 0.11508 15800.00 12800.00 0.00 0.11568 15400.00 13000.00 0.00 0.11590 15600.00 13000.00 0.00 0.11747 15800.00 13000.00 0.00 0.11803 15400.00 13200.00 0.00 0.11855 15600.00 13200.00 0.00 0.12011 15800.00 13200.00 0.00 0.12063 15400.00 13400.00 0.00 0.12130 15600.00 13400.00 0.00 0.12284 15800.00 13400.00 0.00 0.12328 15400.00 13600.00 0.00 0.12402 15600.00 13600.00 0.00 0.12551 15800.00 13600.00 0.00 0.12585 15400.00 13800.00 0.00 0.12672 15600.00 13800.00 0.00 0.12817 15800.00 13800.00 0.00 0.12839 15400.00 14000.00 0.00 0.12930 15600.00 14000.00 0.00 0.13069 15800.00 14000.00 0.00 0.13076 15400.00 14200.00 0.00 0.13204 15600.00 14200.00 0.00 0.13329 15400.00 14400.00 0.00 0.13423 15600.00 14400.00 0.00 0.13534 15400.00 14600.00 0.00 0.13673 15600.00 14600.00 0.00 0.13767 15400.00 14800.00 0.00 0.13923 15600.00 14800.00 0.00 0.13991 15400.00 15000.00 0.00 0.14191 15600.00 15000.00 0.00 0.14214 15200.00 15200.00 0.00 0.14307 15400.00 15200.00 0.00 0.14441 15200.00 15400.00 0.00 0.14451 15400.00 15400.00 0.00 0.14533 15400.00 15600.00 15.00 0.14538 15200.00 15600.00 20.00 0.14548 15200.00 15600.00 25.00 0.14562 15200.00 15600.00 30.00 0.14572 15200.00 15600.00 35.00 0.14580 15200.00 15600.00 40.00 0.14585 15200.00 15600.00 45.00 0.14585 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15274.40 15562.94 40.25 0.14603 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/094.ctf NX, NY, NZ: 512 512 1 MODE : real