CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/093.mrc Output diagnostic file name Micrographs_0601/093.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/093.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2408.000 8168.000 Mean, RMS : 4456.840 216.1737 TITLE 1: emi2mrc: 093.emi Sun Jan 05 14:54:40 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 80.878, Y 311.598, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02310 10200.00 10000.00 0.00 0.02417 10400.00 10000.00 0.00 0.02530 10600.00 10000.00 0.00 0.02681 10800.00 10000.00 0.00 0.02819 11000.00 10000.00 0.00 0.02955 11200.00 10000.00 0.00 0.03072 11400.00 10000.00 0.00 0.03187 11600.00 10000.00 0.00 0.03330 11800.00 10000.00 0.00 0.03485 12000.00 10000.00 0.00 0.03669 12200.00 10000.00 0.00 0.03844 12400.00 10000.00 0.00 0.04018 12600.00 10000.00 0.00 0.04214 12800.00 10000.00 0.00 0.04439 13000.00 10000.00 0.00 0.04680 13200.00 10000.00 0.00 0.04924 13400.00 10000.00 0.00 0.05190 13600.00 10000.00 0.00 0.05448 13800.00 10000.00 0.00 0.05690 14000.00 10000.00 0.00 0.05922 14200.00 10000.00 0.00 0.06161 14400.00 10000.00 0.00 0.06415 14600.00 10000.00 0.00 0.06673 14800.00 10000.00 0.00 0.06945 15000.00 10000.00 0.00 0.07244 15200.00 10000.00 0.00 0.07550 15400.00 10000.00 0.00 0.07826 15600.00 10000.00 0.00 0.08067 15800.00 10000.00 0.00 0.08227 16000.00 10000.00 0.00 0.08300 16200.00 10000.00 0.00 0.08312 15800.00 10200.00 0.00 0.08410 16000.00 10200.00 0.00 0.08482 16200.00 10200.00 0.00 0.08484 15800.00 10400.00 0.00 0.08619 16000.00 10400.00 0.00 0.08683 15800.00 10600.00 0.00 0.08826 16000.00 10600.00 0.00 0.08880 15800.00 10800.00 0.00 0.09025 16000.00 10800.00 0.00 0.09072 15600.00 11000.00 0.00 0.09083 15800.00 11000.00 0.00 0.09237 16000.00 11000.00 0.00 0.09280 15800.00 11200.00 0.00 0.09432 16000.00 11200.00 0.00 0.09475 15600.00 11400.00 0.00 0.09500 15800.00 11400.00 0.00 0.09657 16000.00 11400.00 0.00 0.09699 15600.00 11600.00 0.00 0.09729 15800.00 11600.00 0.00 0.09887 16000.00 11600.00 0.00 0.09920 15600.00 11800.00 0.00 0.09979 15800.00 11800.00 0.00 0.10130 16000.00 11800.00 0.00 0.10153 15600.00 12000.00 0.00 0.10231 15800.00 12000.00 0.00 0.10375 16000.00 12000.00 0.00 0.10390 15600.00 12200.00 0.00 0.10484 15800.00 12200.00 0.00 0.10619 16000.00 12200.00 0.00 0.10624 15600.00 12400.00 0.00 0.10760 15800.00 12400.00 0.00 0.10882 15600.00 12600.00 0.00 0.11018 15800.00 12600.00 0.00 0.11133 15600.00 12800.00 0.00 0.11301 15800.00 12800.00 0.00 0.11414 15600.00 13000.00 0.00 0.11575 15800.00 13000.00 0.00 0.11687 15600.00 13200.00 0.00 0.11871 15800.00 13200.00 0.00 0.11980 15600.00 13400.00 0.00 0.12178 15800.00 13400.00 0.00 0.12283 15600.00 13600.00 0.00 0.12485 15800.00 13600.00 0.00 0.12586 15600.00 13800.00 0.00 0.12798 15800.00 13800.00 0.00 0.12890 15600.00 14000.00 0.00 0.13091 15800.00 14000.00 0.00 0.13171 15600.00 14200.00 0.00 0.13408 15800.00 14200.00 0.00 0.13475 15400.00 14400.00 0.00 0.13478 15600.00 14400.00 0.00 0.13714 15800.00 14400.00 0.00 0.13765 15400.00 14600.00 0.00 0.13835 15600.00 14600.00 0.00 0.14063 15800.00 14600.00 0.00 0.14089 15400.00 14800.00 0.00 0.14187 15600.00 14800.00 0.00 0.14398 15400.00 15000.00 0.00 0.14537 15600.00 15000.00 0.00 0.14717 15400.00 15200.00 0.00 0.14846 15600.00 15200.00 0.00 0.14984 15400.00 15400.00 0.00 0.15018 15600.00 15400.00 0.00 0.15117 15600.00 15600.00 0.00 0.15120 15400.00 15600.00 15.00 0.15121 15400.00 15600.00 20.00 0.15130 15400.00 15600.00 25.00 0.15138 15400.00 15600.00 30.00 0.15145 15400.00 15600.00 35.00 0.15151 15400.00 15600.00 40.00 0.15157 15400.00 15600.00 45.00 0.15160 15400.00 15600.00 50.00 0.15163 15400.00 15600.00 55.00 0.15163 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15400.00 15600.00 53.51 0.15163 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/093.ctf NX, NY, NZ: 512 512 1 MODE : real