CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/092.mrc Output diagnostic file name Micrographs_0601/092.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/092.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1900.000 8304.000 Mean, RMS : 4474.091 217.5935 TITLE 1: emi2mrc: 092.emi Sun Jan 05 14:53:43 2014 TITLE 2: kV 300, Mag 78000x, Def 20180, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 78.378, Y 312.171, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03599 10200.00 10000.00 0.00 0.03796 10400.00 10000.00 0.00 0.03975 10600.00 10000.00 0.00 0.04169 10800.00 10000.00 0.00 0.04364 11000.00 10000.00 0.00 0.04589 11200.00 10000.00 0.00 0.04839 11400.00 10000.00 0.00 0.05078 11600.00 10000.00 0.00 0.05307 11800.00 10000.00 0.00 0.05539 12000.00 10000.00 0.00 0.05796 12200.00 10000.00 0.00 0.06052 12400.00 10000.00 0.00 0.06329 12600.00 10000.00 0.00 0.06621 12800.00 10000.00 0.00 0.06920 13000.00 10000.00 0.00 0.07218 13200.00 10000.00 0.00 0.07513 13400.00 10000.00 0.00 0.07819 13600.00 10000.00 0.00 0.08123 13800.00 10000.00 0.00 0.08453 14000.00 10000.00 0.00 0.08774 14200.00 10000.00 0.00 0.09069 14400.00 10000.00 0.00 0.09314 14600.00 10000.00 0.00 0.09478 14800.00 10000.00 0.00 0.09554 15000.00 10000.00 0.00 0.09572 14600.00 10200.00 0.00 0.09695 14800.00 10200.00 0.00 0.09767 15000.00 10200.00 0.00 0.09784 14600.00 10400.00 0.00 0.09940 14800.00 10400.00 0.00 0.10007 15000.00 10400.00 0.00 0.10022 14400.00 10600.00 0.00 0.10038 14600.00 10600.00 0.00 0.10186 14800.00 10600.00 0.00 0.10247 15000.00 10600.00 0.00 0.10258 14400.00 10800.00 0.00 0.10278 14600.00 10800.00 0.00 0.10417 14800.00 10800.00 0.00 0.10476 15000.00 10800.00 0.00 0.10482 14400.00 11000.00 0.00 0.10548 14600.00 11000.00 0.00 0.10683 14800.00 11000.00 0.00 0.10741 15000.00 11000.00 0.00 0.10745 14400.00 11200.00 0.00 0.10811 14600.00 11200.00 0.00 0.10945 14800.00 11200.00 0.00 0.11007 14400.00 11400.00 0.00 0.11092 14600.00 11400.00 0.00 0.11229 14800.00 11400.00 0.00 0.11287 14400.00 11600.00 0.00 0.11374 14600.00 11600.00 0.00 0.11508 14800.00 11600.00 0.00 0.11558 14400.00 11800.00 0.00 0.11677 14600.00 11800.00 0.00 0.11805 14800.00 11800.00 0.00 0.11843 14400.00 12000.00 0.00 0.11984 14600.00 12000.00 0.00 0.12107 14800.00 12000.00 0.00 0.12139 14400.00 12200.00 0.00 0.12295 14600.00 12200.00 0.00 0.12415 14800.00 12200.00 0.00 0.12439 14400.00 12400.00 0.00 0.12631 14600.00 12400.00 0.00 0.12741 14800.00 12400.00 0.00 0.12754 14400.00 12600.00 0.00 0.12944 14600.00 12600.00 0.00 0.13043 14800.00 12600.00 0.00 0.13044 14200.00 12800.00 0.00 0.13069 14400.00 12800.00 0.00 0.13288 14600.00 12800.00 0.00 0.13374 14200.00 13000.00 0.00 0.13401 14400.00 13000.00 0.00 0.13612 14600.00 13000.00 0.00 0.13687 14200.00 13200.00 0.00 0.13752 14400.00 13200.00 0.00 0.13957 14600.00 13200.00 0.00 0.14019 14200.00 13400.00 0.00 0.14108 14400.00 13400.00 0.00 0.14304 14600.00 13400.00 0.00 0.14347 14200.00 13600.00 0.00 0.14477 14400.00 13600.00 0.00 0.14658 14600.00 13600.00 0.00 0.14672 14200.00 13800.00 0.00 0.14856 14400.00 13800.00 0.00 0.15010 14200.00 14000.00 0.00 0.15175 14400.00 14000.00 0.00 0.15296 14200.00 14200.00 0.00 0.15486 14400.00 14200.00 0.00 0.15529 14200.00 14400.00 0.00 0.15568 14200.00 14400.00 5.00 0.15578 14200.00 14400.00 10.00 0.15587 14200.00 14400.00 15.00 0.15595 14200.00 14400.00 20.00 0.15601 14200.00 14400.00 25.00 0.15607 14200.00 14400.00 30.00 0.15610 14200.00 14400.00 35.00 0.15611 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14247.30 14353.22 35.00 0.15621 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/092.ctf NX, NY, NZ: 512 512 1 MODE : real