CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/091.mrc Output diagnostic file name Micrographs_0601/091.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/091.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2460.000 8466.000 Mean, RMS : 4469.214 216.0611 TITLE 1: emi2mrc: 091.emi Sun Jan 05 14:52:39 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 75.777, Y 312.430, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.05290 10200.00 10000.00 0.00 0.05546 10400.00 10000.00 0.00 0.05775 10600.00 10000.00 0.00 0.06049 10800.00 10000.00 0.00 0.06330 11000.00 10000.00 0.00 0.06634 11200.00 10000.00 0.00 0.06961 11400.00 10000.00 0.00 0.07293 11600.00 10000.00 0.00 0.07623 11800.00 10000.00 0.00 0.07958 12000.00 10000.00 0.00 0.08325 12200.00 10000.00 0.00 0.08687 12400.00 10000.00 0.00 0.09068 12600.00 10000.00 0.00 0.09443 12800.00 10000.00 0.00 0.09839 13000.00 10000.00 0.00 0.10223 13200.00 10000.00 0.00 0.10523 13400.00 10000.00 0.00 0.10729 13600.00 10000.00 0.00 0.10800 13800.00 10000.00 0.00 0.10811 13200.00 10200.00 0.00 0.10822 13400.00 10200.00 0.00 0.11023 13600.00 10200.00 0.00 0.11085 13800.00 10200.00 0.00 0.11087 13200.00 10400.00 0.00 0.11126 13400.00 10400.00 0.00 0.11317 13600.00 10400.00 0.00 0.11371 13200.00 10600.00 0.00 0.11444 13400.00 10600.00 0.00 0.11633 13600.00 10600.00 0.00 0.11683 13200.00 10800.00 0.00 0.11777 13400.00 10800.00 0.00 0.11965 13600.00 10800.00 0.00 0.12011 13200.00 11000.00 0.00 0.12138 13400.00 11000.00 0.00 0.12325 13600.00 11000.00 0.00 0.12362 13200.00 11200.00 0.00 0.12493 13400.00 11200.00 0.00 0.12679 13600.00 11200.00 0.00 0.12710 13200.00 11400.00 0.00 0.12896 13400.00 11400.00 0.00 0.13077 13600.00 11400.00 0.00 0.13096 13200.00 11600.00 0.00 0.13300 13400.00 11600.00 0.00 0.13471 13600.00 11600.00 0.00 0.13471 13200.00 11800.00 0.00 0.13724 13400.00 11800.00 0.00 0.13883 13200.00 12000.00 0.00 0.14143 13400.00 12000.00 0.00 0.14286 13000.00 12200.00 0.00 0.14290 13200.00 12200.00 0.00 0.14549 13400.00 12200.00 0.00 0.14669 13000.00 12400.00 0.00 0.14730 13200.00 12400.00 0.00 0.14962 13400.00 12400.00 0.00 0.15051 13000.00 12600.00 0.00 0.15118 13200.00 12600.00 0.00 0.15320 13400.00 12600.00 0.00 0.15382 13000.00 12800.00 0.00 0.15553 13200.00 12800.00 0.00 0.15705 13400.00 12800.00 0.00 0.15727 13000.00 13000.00 0.00 0.15840 13200.00 13000.00 0.00 0.15953 13000.00 13200.00 0.00 0.16003 13200.00 13200.00 0.00 0.16102 13000.00 13400.00 25.00 0.16103 13000.00 13400.00 30.00 0.16109 13000.00 13400.00 35.00 0.16110 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13150.73 13318.88 35.00 0.16146 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/091.ctf NX, NY, NZ: 512 512 1 MODE : real