CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/088.mrc Output diagnostic file name Micrographs_0601/088.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/088.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2338.000 8172.000 Mean, RMS : 4447.635 213.4088 TITLE 1: emi2mrc: 088.emi Sun Jan 05 14:49:43 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.10%, Obj 88.08%, Dif 73.67% TITLE 3: X 78.081, Y 315.046, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.05569 10200.00 10000.00 0.00 0.05850 10400.00 10000.00 0.00 0.06080 10600.00 10000.00 0.00 0.06373 10800.00 10000.00 0.00 0.06688 11000.00 10000.00 0.00 0.07006 11200.00 10000.00 0.00 0.07324 11400.00 10000.00 0.00 0.07640 11600.00 10000.00 0.00 0.07978 11800.00 10000.00 0.00 0.08305 12000.00 10000.00 0.00 0.08650 12200.00 10000.00 0.00 0.08992 12400.00 10000.00 0.00 0.09352 12600.00 10000.00 0.00 0.09716 12800.00 10000.00 0.00 0.10076 13000.00 10000.00 0.00 0.10411 13200.00 10000.00 0.00 0.10657 13400.00 10000.00 0.00 0.10812 13600.00 10000.00 0.00 0.10850 13200.00 10200.00 0.00 0.10949 13400.00 10200.00 0.00 0.11095 13600.00 10200.00 0.00 0.11123 13200.00 10400.00 0.00 0.11258 13400.00 10400.00 0.00 0.11396 13600.00 10400.00 0.00 0.11420 13200.00 10600.00 0.00 0.11581 13400.00 10600.00 0.00 0.11713 13600.00 10600.00 0.00 0.11733 13200.00 10800.00 0.00 0.11888 13400.00 10800.00 0.00 0.12015 13600.00 10800.00 0.00 0.12028 13200.00 11000.00 0.00 0.12214 13400.00 11000.00 0.00 0.12336 13200.00 11200.00 0.00 0.12513 13400.00 11200.00 0.00 0.12628 13200.00 11400.00 0.00 0.12845 13400.00 11400.00 0.00 0.12947 13000.00 11600.00 0.00 0.12956 13200.00 11600.00 0.00 0.13166 13400.00 11600.00 0.00 0.13252 13000.00 11800.00 0.00 0.13298 13200.00 11800.00 0.00 0.13490 13400.00 11800.00 0.00 0.13558 13000.00 12000.00 0.00 0.13639 13200.00 12000.00 0.00 0.13813 13400.00 12000.00 0.00 0.13863 13000.00 12200.00 0.00 0.13971 13200.00 12200.00 0.00 0.14126 13400.00 12200.00 0.00 0.14152 13000.00 12400.00 0.00 0.14325 13200.00 12400.00 0.00 0.14451 13000.00 12600.00 0.00 0.14618 13200.00 12600.00 0.00 0.14709 13000.00 12800.00 0.00 0.14938 13200.00 12800.00 0.00 0.14968 13000.00 13000.00 0.00 0.15096 13000.00 13200.00 0.00 0.15101 13000.00 13200.00 5.00 0.15104 13000.00 13200.00 10.00 0.15106 13000.00 13200.00 15.00 0.15108 13000.00 13200.00 20.00 0.15110 13000.00 13200.00 25.00 0.15110 13000.00 13200.00 30.00 0.15110 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12932.01 13156.59 39.48 0.15123 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/088.ctf NX, NY, NZ: 512 512 1 MODE : real