CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/087.mrc Output diagnostic file name Micrographs_0601/087.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/087.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2242.000 7832.000 Mean, RMS : 4454.278 219.2013 TITLE 1: emi2mrc: 087.emi Sun Jan 05 14:49:06 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.10%, Obj 88.08%, Dif 73.67% TITLE 3: X 80.685, Y 314.881, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03779 10200.00 10000.00 0.00 0.03960 10400.00 10000.00 0.00 0.04118 10600.00 10000.00 0.00 0.04305 10800.00 10000.00 0.00 0.04477 11000.00 10000.00 0.00 0.04672 11200.00 10000.00 0.00 0.04872 11400.00 10000.00 0.00 0.05073 11600.00 10000.00 0.00 0.05307 11800.00 10000.00 0.00 0.05544 12000.00 10000.00 0.00 0.05800 12200.00 10000.00 0.00 0.06062 12400.00 10000.00 0.00 0.06339 12600.00 10000.00 0.00 0.06615 12800.00 10000.00 0.00 0.06890 13000.00 10000.00 0.00 0.07167 13200.00 10000.00 0.00 0.07453 13400.00 10000.00 0.00 0.07776 13600.00 10000.00 0.00 0.08103 13800.00 10000.00 0.00 0.08440 14000.00 10000.00 0.00 0.08779 14200.00 10000.00 0.00 0.09135 14400.00 10000.00 0.00 0.09464 14600.00 10000.00 0.00 0.09677 14800.00 10000.00 0.00 0.09764 15000.00 10000.00 0.00 0.09781 15200.00 10000.00 0.00 0.09781 14600.00 10200.00 0.00 0.09874 14800.00 10200.00 0.00 0.09963 15000.00 10200.00 0.00 0.09980 14600.00 10400.00 0.00 0.10083 14800.00 10400.00 0.00 0.10173 15000.00 10400.00 0.00 0.10185 14600.00 10600.00 0.00 0.10293 14800.00 10600.00 0.00 0.10383 15000.00 10600.00 0.00 0.10391 14600.00 10800.00 0.00 0.10517 14800.00 10800.00 0.00 0.10608 15000.00 10800.00 0.00 0.10614 14600.00 11000.00 0.00 0.10766 14800.00 11000.00 0.00 0.10857 15000.00 11000.00 0.00 0.10858 14600.00 11200.00 0.00 0.11015 14800.00 11200.00 0.00 0.11105 14600.00 11400.00 0.00 0.11302 14800.00 11400.00 0.00 0.11389 14600.00 11600.00 0.00 0.11582 14800.00 11600.00 0.00 0.11669 14400.00 11800.00 0.00 0.11672 14600.00 11800.00 0.00 0.11898 14800.00 11800.00 0.00 0.11977 14400.00 12000.00 0.00 0.12002 14600.00 12000.00 0.00 0.12220 14800.00 12000.00 0.00 0.12285 14400.00 12200.00 0.00 0.12326 14600.00 12200.00 0.00 0.12540 14800.00 12200.00 0.00 0.12596 14400.00 12400.00 0.00 0.12677 14600.00 12400.00 0.00 0.12888 14800.00 12400.00 0.00 0.12935 14400.00 12600.00 0.00 0.13009 14600.00 12600.00 0.00 0.13215 14800.00 12600.00 0.00 0.13250 14400.00 12800.00 0.00 0.13378 14600.00 12800.00 0.00 0.13576 14800.00 12800.00 0.00 0.13595 14400.00 13000.00 0.00 0.13731 14600.00 13000.00 0.00 0.13919 14800.00 13000.00 0.00 0.13921 14400.00 13200.00 0.00 0.14107 14600.00 13200.00 0.00 0.14280 14400.00 13400.00 0.00 0.14480 14600.00 13400.00 0.00 0.14639 14400.00 13600.00 0.00 0.14884 14600.00 13600.00 0.00 0.15022 14400.00 13800.00 0.00 0.15316 14600.00 13800.00 0.00 0.15419 14400.00 14000.00 0.00 0.15709 14600.00 14000.00 0.00 0.15766 14200.00 14200.00 0.00 0.15867 14400.00 14200.00 0.00 0.16144 14200.00 14400.00 0.00 0.16216 14400.00 14400.00 0.00 0.16375 14400.00 14600.00 0.00 0.16390 14400.00 14600.00 5.00 0.16401 14400.00 14600.00 10.00 0.16410 14400.00 14600.00 15.00 0.16417 14400.00 14600.00 20.00 0.16421 14400.00 14600.00 25.00 0.16423 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14311.81 14569.62 28.76 0.16456 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/087.ctf NX, NY, NZ: 512 512 1 MODE : real