CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/086.mrc Output diagnostic file name Micrographs_0601/086.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/086.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2544.000 8828.000 Mean, RMS : 4485.661 222.0940 TITLE 1: emi2mrc: 086.emi Sun Jan 05 14:48:12 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 83.391, Y 314.297, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00039 10200.00 10000.00 0.00 0.00050 10400.00 10000.00 0.00 0.00060 10600.00 10000.00 0.00 0.00070 10800.00 10000.00 0.00 0.00094 11000.00 10000.00 0.00 0.00114 11200.00 10000.00 0.00 0.00117 11400.00 10000.00 0.00 0.00123 11600.00 10000.00 0.00 0.00140 11800.00 10000.00 0.00 0.00168 12000.00 10000.00 0.00 0.00211 12200.00 10000.00 0.00 0.00247 12400.00 10000.00 0.00 0.00275 12600.00 10000.00 0.00 0.00308 12800.00 10000.00 0.00 0.00344 13000.00 10000.00 0.00 0.00386 13200.00 10000.00 0.00 0.00424 13400.00 10000.00 0.00 0.00458 13600.00 10000.00 0.00 0.00509 13800.00 10000.00 0.00 0.00572 14000.00 10000.00 0.00 0.00627 14200.00 10000.00 0.00 0.00678 14400.00 10000.00 0.00 0.00746 14600.00 10000.00 0.00 0.00829 14800.00 10000.00 0.00 0.00907 15000.00 10000.00 0.00 0.00973 15200.00 10000.00 0.00 0.01041 15400.00 10000.00 0.00 0.01115 15600.00 10000.00 0.00 0.01193 15800.00 10000.00 0.00 0.01277 16000.00 10000.00 0.00 0.01376 16200.00 10000.00 0.00 0.01488 16400.00 10000.00 0.00 0.01608 16600.00 10000.00 0.00 0.01724 16800.00 10000.00 0.00 0.01838 17000.00 10000.00 0.00 0.01967 17200.00 10000.00 0.00 0.02096 17400.00 10000.00 0.00 0.02211 17600.00 10000.00 0.00 0.02319 17800.00 10000.00 0.00 0.02448 18000.00 10000.00 0.00 0.02583 18200.00 10000.00 0.00 0.02704 18400.00 10000.00 0.00 0.02830 18600.00 10000.00 0.00 0.02966 18800.00 10000.00 0.00 0.03109 19000.00 10000.00 0.00 0.03253 19200.00 10000.00 0.00 0.03412 19400.00 10000.00 0.00 0.03583 19600.00 10000.00 0.00 0.03746 19800.00 10000.00 0.00 0.03904 20000.00 10000.00 0.00 0.04066 20200.00 10000.00 0.00 0.04251 20400.00 10000.00 0.00 0.04438 20600.00 10000.00 0.00 0.04617 20800.00 10000.00 0.00 0.04807 21000.00 10000.00 0.00 0.05013 21200.00 10000.00 0.00 0.05206 21400.00 10000.00 0.00 0.05334 21600.00 10000.00 0.00 0.05407 21800.00 10000.00 0.00 0.05446 22000.00 10000.00 0.00 0.05462 21600.00 10200.00 0.00 0.05470 21800.00 10200.00 0.00 0.05513 22000.00 10200.00 0.00 0.05530 21600.00 10400.00 0.00 0.05538 21800.00 10400.00 0.00 0.05579 22000.00 10400.00 0.00 0.05590 21600.00 10600.00 0.00 0.05602 21800.00 10600.00 0.00 0.05638 22000.00 10600.00 0.00 0.05649 21600.00 10800.00 0.00 0.05666 21800.00 10800.00 0.00 0.05704 22000.00 10800.00 0.00 0.05717 21600.00 11000.00 0.00 0.05743 21800.00 11000.00 0.00 0.05785 22000.00 11000.00 0.00 0.05797 21600.00 11200.00 0.00 0.05811 21800.00 11200.00 0.00 0.05851 22000.00 11200.00 0.00 0.05860 21600.00 11400.00 0.00 0.05874 21800.00 11400.00 0.00 0.05914 22000.00 11400.00 0.00 0.05921 21600.00 11600.00 0.00 0.05936 21800.00 11600.00 0.00 0.05978 22000.00 11600.00 0.00 0.05985 21600.00 11800.00 0.00 0.06009 21800.00 11800.00 0.00 0.06050 22000.00 11800.00 0.00 0.06051 21600.00 12000.00 0.00 0.06085 21800.00 12000.00 0.00 0.06121 21600.00 12200.00 0.00 0.06154 21800.00 12200.00 0.00 0.06185 21600.00 12400.00 0.00 0.06227 21800.00 12400.00 0.00 0.06258 21600.00 12600.00 0.00 0.06313 21800.00 12600.00 0.00 0.06347 22000.00 12600.00 0.00 0.06353 21600.00 12800.00 0.00 0.06414 21800.00 12800.00 0.00 0.06451 22000.00 12800.00 0.00 0.06458 21600.00 13000.00 0.00 0.06512 21800.00 13000.00 0.00 0.06552 22000.00 13000.00 0.00 0.06558 21600.00 13200.00 0.00 0.06639 21800.00 13200.00 0.00 0.06676 21400.00 13400.00 0.00 0.06677 21600.00 13400.00 0.00 0.06765 21800.00 13400.00 0.00 0.06793 21600.00 13600.00 0.00 0.06865 21800.00 13600.00 0.00 0.06889 21400.00 13800.00 0.00 0.06897 21600.00 13800.00 0.00 0.06979 21800.00 13800.00 0.00 0.07006 21400.00 14000.00 0.00 0.07022 21600.00 14000.00 0.00 0.07105 21800.00 14000.00 0.00 0.07133 21400.00 14200.00 0.00 0.07154 21600.00 14200.00 0.00 0.07236 21800.00 14200.00 0.00 0.07261 21400.00 14400.00 0.00 0.07280 21600.00 14400.00 0.00 0.07363 21800.00 14400.00 0.00 0.07387 21400.00 14600.00 0.00 0.07420 21600.00 14600.00 0.00 0.07502 21800.00 14600.00 0.00 0.07524 21400.00 14800.00 0.00 0.07562 21600.00 14800.00 0.00 0.07643 21800.00 14800.00 0.00 0.07660 21400.00 15000.00 0.00 0.07709 21600.00 15000.00 0.00 0.07787 21800.00 15000.00 0.00 0.07800 21400.00 15200.00 0.00 0.07861 21600.00 15200.00 0.00 0.07935 21800.00 15200.00 0.00 0.07945 21400.00 15400.00 0.00 0.08007 21600.00 15400.00 0.00 0.08078 21800.00 15400.00 0.00 0.08087 21400.00 15600.00 0.00 0.08162 21600.00 15600.00 0.00 0.08231 21800.00 15600.00 0.00 0.08240 21400.00 15800.00 0.00 0.08316 21600.00 15800.00 0.00 0.08382 21800.00 15800.00 0.00 0.08390 21400.00 16000.00 0.00 0.08474 21600.00 16000.00 0.00 0.08539 21800.00 16000.00 0.00 0.08546 21400.00 16200.00 0.00 0.08634 21600.00 16200.00 0.00 0.08701 21800.00 16200.00 0.00 0.08708 21400.00 16400.00 0.00 0.08805 21600.00 16400.00 0.00 0.08874 21800.00 16400.00 0.00 0.08877 21400.00 16600.00 0.00 0.08978 21600.00 16600.00 0.00 0.09042 21400.00 16800.00 0.00 0.09143 21600.00 16800.00 0.00 0.09199 21400.00 17000.00 0.00 0.09314 21600.00 17000.00 0.00 0.09365 21400.00 17200.00 0.00 0.09485 21600.00 17200.00 0.00 0.09533 21400.00 17400.00 0.00 0.09658 21600.00 17400.00 0.00 0.09705 21400.00 17600.00 0.00 0.09844 21600.00 17600.00 0.00 0.09890 21200.00 17800.00 0.00 0.09892 21400.00 17800.00 0.00 0.10048 21600.00 17800.00 0.00 0.10090 21200.00 18000.00 0.00 0.10090 21400.00 18000.00 0.00 0.10244 21600.00 18000.00 0.00 0.10279 21200.00 18200.00 0.00 0.10297 21400.00 18200.00 0.00 0.10445 21600.00 18200.00 0.00 0.10470 21200.00 18400.00 0.00 0.10495 21400.00 18400.00 0.00 0.10635 21600.00 18400.00 0.00 0.10652 21200.00 18600.00 0.00 0.10691 21400.00 18600.00 0.00 0.10822 21600.00 18600.00 0.00 0.10830 21200.00 18800.00 0.00 0.10903 21400.00 18800.00 0.00 0.11024 21200.00 19000.00 0.00 0.11107 21400.00 19000.00 0.00 0.11223 21200.00 19200.00 0.00 0.11334 21400.00 19200.00 0.00 0.11445 21200.00 19400.00 0.00 0.11559 21400.00 19400.00 0.00 0.11664 21200.00 19600.00 0.00 0.11794 21400.00 19600.00 0.00 0.11893 21200.00 19800.00 0.00 0.12034 21400.00 19800.00 0.00 0.12126 21200.00 20000.00 0.00 0.12293 21400.00 20000.00 0.00 0.12375 21200.00 20200.00 0.00 0.12567 21400.00 20200.00 0.00 0.12633 21200.00 20400.00 0.00 0.12837 21400.00 20400.00 0.00 0.12883 21200.00 20600.00 0.00 0.13134 21400.00 20600.00 0.00 0.13146 21200.00 20800.00 0.00 0.13390 21000.00 21000.00 0.00 0.13436 21200.00 21000.00 0.00 0.13643 21000.00 21200.00 0.00 0.13664 21200.00 21200.00 0.00 0.13786 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21122.55 21292.40 33.00 0.13817 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/086.ctf NX, NY, NZ: 512 512 1 MODE : real