CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/084.mrc Output diagnostic file name Micrographs_0601/084.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/084.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2312.000 8732.000 Mean, RMS : 4471.724 217.0007 TITLE 1: emi2mrc: 084.emi Sun Jan 05 14:46:34 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 88.664, Y 313.388, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04674 10200.00 10000.00 0.00 0.04924 10400.00 10000.00 0.00 0.05154 10600.00 10000.00 0.00 0.05425 10800.00 10000.00 0.00 0.05695 11000.00 10000.00 0.00 0.05989 11200.00 10000.00 0.00 0.06294 11400.00 10000.00 0.00 0.06620 11600.00 10000.00 0.00 0.06967 11800.00 10000.00 0.00 0.07299 12000.00 10000.00 0.00 0.07665 12200.00 10000.00 0.00 0.08030 12400.00 10000.00 0.00 0.08387 12600.00 10000.00 0.00 0.08728 12800.00 10000.00 0.00 0.09082 13000.00 10000.00 0.00 0.09463 13200.00 10000.00 0.00 0.09843 13400.00 10000.00 0.00 0.10237 13600.00 10000.00 0.00 0.10542 13800.00 10000.00 0.00 0.10667 14000.00 10000.00 0.00 0.10670 13600.00 10200.00 0.00 0.10819 13800.00 10200.00 0.00 0.10943 13600.00 10400.00 0.00 0.11101 13800.00 10400.00 0.00 0.11221 13600.00 10600.00 0.00 0.11399 13800.00 10600.00 0.00 0.11513 13600.00 10800.00 0.00 0.11693 13800.00 10800.00 0.00 0.11798 13600.00 11000.00 0.00 0.12014 13800.00 11000.00 0.00 0.12106 13600.00 11200.00 0.00 0.12300 13800.00 11200.00 0.00 0.12381 13600.00 11400.00 0.00 0.12607 13800.00 11400.00 0.00 0.12677 13600.00 11600.00 0.00 0.12928 13800.00 11600.00 0.00 0.12987 13600.00 11800.00 0.00 0.13265 13800.00 11800.00 0.00 0.13305 13400.00 12000.00 0.00 0.13306 13600.00 12000.00 0.00 0.13590 13800.00 12000.00 0.00 0.13607 13400.00 12200.00 0.00 0.13626 13600.00 12200.00 0.00 0.13895 13400.00 12400.00 0.00 0.13955 13600.00 12400.00 0.00 0.14206 13400.00 12600.00 0.00 0.14244 13600.00 12600.00 0.00 0.14481 13400.00 12800.00 0.00 0.14574 13600.00 12800.00 0.00 0.14791 13400.00 13000.00 0.00 0.14854 13600.00 13000.00 0.00 0.15047 13400.00 13200.00 0.00 0.15139 13600.00 13200.00 0.00 0.15291 13400.00 13400.00 0.00 0.15423 13600.00 13400.00 0.00 0.15498 13400.00 13600.00 0.00 0.15593 13600.00 13600.00 0.00 0.15617 13400.00 13800.00 0.00 0.15639 13400.00 13800.00 5.00 0.15650 13400.00 13800.00 10.00 0.15655 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13471.06 13706.18 9.86 0.15690 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/084.ctf NX, NY, NZ: 512 512 1 MODE : real