CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/083.mrc Output diagnostic file name Micrographs_0601/083.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/083.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2526.000 8438.000 Mean, RMS : 4477.083 220.1946 TITLE 1: emi2mrc: 083.emi Sun Jan 05 14:45:56 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 89.179, Y 316.269, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03006 10200.00 10000.00 0.00 0.03153 10400.00 10000.00 0.00 0.03283 10600.00 10000.00 0.00 0.03439 10800.00 10000.00 0.00 0.03593 11000.00 10000.00 0.00 0.03765 11200.00 10000.00 0.00 0.03961 11400.00 10000.00 0.00 0.04162 11600.00 10000.00 0.00 0.04373 11800.00 10000.00 0.00 0.04576 12000.00 10000.00 0.00 0.04792 12200.00 10000.00 0.00 0.05019 12400.00 10000.00 0.00 0.05263 12600.00 10000.00 0.00 0.05513 12800.00 10000.00 0.00 0.05778 13000.00 10000.00 0.00 0.06059 13200.00 10000.00 0.00 0.06341 13400.00 10000.00 0.00 0.06640 13600.00 10000.00 0.00 0.06945 13800.00 10000.00 0.00 0.07266 14000.00 10000.00 0.00 0.07604 14200.00 10000.00 0.00 0.07969 14400.00 10000.00 0.00 0.08340 14600.00 10000.00 0.00 0.08733 14800.00 10000.00 0.00 0.09093 15000.00 10000.00 0.00 0.09291 15200.00 10000.00 0.00 0.09347 15000.00 10200.00 0.00 0.09471 15200.00 10200.00 0.00 0.09524 15000.00 10400.00 0.00 0.09664 15200.00 10400.00 0.00 0.09714 15000.00 10600.00 0.00 0.09878 15200.00 10600.00 0.00 0.09920 15000.00 10800.00 0.00 0.10087 15200.00 10800.00 0.00 0.10123 14800.00 11000.00 0.00 0.10132 15000.00 11000.00 0.00 0.10321 15200.00 11000.00 0.00 0.10350 14800.00 11200.00 0.00 0.10369 15000.00 11200.00 0.00 0.10552 15200.00 11200.00 0.00 0.10573 14800.00 11400.00 0.00 0.10634 15000.00 11400.00 0.00 0.10812 15200.00 11400.00 0.00 0.10823 14800.00 11600.00 0.00 0.10918 15000.00 11600.00 0.00 0.11090 15200.00 11600.00 0.00 0.11093 14800.00 11800.00 0.00 0.11229 15000.00 11800.00 0.00 0.11395 14800.00 12000.00 0.00 0.11552 15000.00 12000.00 0.00 0.11714 14800.00 12200.00 0.00 0.11880 15000.00 12200.00 0.00 0.12038 14800.00 12400.00 0.00 0.12222 15000.00 12400.00 0.00 0.12373 14800.00 12600.00 0.00 0.12543 15000.00 12600.00 0.00 0.12688 14800.00 12800.00 0.00 0.12903 15000.00 12800.00 0.00 0.13041 14800.00 13000.00 0.00 0.13268 15000.00 13000.00 0.00 0.13398 14800.00 13200.00 0.00 0.13655 15000.00 13200.00 0.00 0.13769 14800.00 13400.00 0.00 0.14033 15000.00 13400.00 0.00 0.14127 14800.00 13600.00 0.00 0.14408 15000.00 13600.00 0.00 0.14482 14800.00 13800.00 0.00 0.14805 15000.00 13800.00 0.00 0.14850 14800.00 14000.00 0.00 0.15207 14600.00 14200.00 0.00 0.15257 14800.00 14200.00 0.00 0.15648 14600.00 14400.00 0.00 0.15669 14800.00 14400.00 0.00 0.16036 14600.00 14600.00 0.00 0.16160 14800.00 14600.00 0.00 0.16455 14600.00 14800.00 0.00 0.16530 14800.00 14800.00 0.00 0.16657 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14713.08 14836.11 26.19 0.16714 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/083.ctf NX, NY, NZ: 512 512 1 MODE : real