CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/081.mrc Output diagnostic file name Micrographs_0601/081.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/081.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2648.000 8032.000 Mean, RMS : 4479.475 222.5848 TITLE 1: emi2mrc: 081.emi Sun Jan 05 14:44:19 2014 TITLE 2: kV 300, Mag 78000x, Def 20020, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X 84.223, Y 316.761, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00450 10200.00 10000.00 0.00 0.00504 10400.00 10000.00 0.00 0.00547 10600.00 10000.00 0.00 0.00594 10800.00 10000.00 0.00 0.00634 11000.00 10000.00 0.00 0.00685 11200.00 10000.00 0.00 0.00756 11400.00 10000.00 0.00 0.00831 11600.00 10000.00 0.00 0.00907 11800.00 10000.00 0.00 0.00979 12000.00 10000.00 0.00 0.01054 12200.00 10000.00 0.00 0.01125 12400.00 10000.00 0.00 0.01191 12600.00 10000.00 0.00 0.01253 12800.00 10000.00 0.00 0.01329 13000.00 10000.00 0.00 0.01415 13200.00 10000.00 0.00 0.01503 13400.00 10000.00 0.00 0.01604 13600.00 10000.00 0.00 0.01695 13800.00 10000.00 0.00 0.01779 14000.00 10000.00 0.00 0.01879 14200.00 10000.00 0.00 0.01998 14400.00 10000.00 0.00 0.02131 14600.00 10000.00 0.00 0.02266 14800.00 10000.00 0.00 0.02398 15000.00 10000.00 0.00 0.02535 15200.00 10000.00 0.00 0.02681 15400.00 10000.00 0.00 0.02819 15600.00 10000.00 0.00 0.02963 15800.00 10000.00 0.00 0.03114 16000.00 10000.00 0.00 0.03264 16200.00 10000.00 0.00 0.03419 16400.00 10000.00 0.00 0.03578 16600.00 10000.00 0.00 0.03747 16800.00 10000.00 0.00 0.03917 17000.00 10000.00 0.00 0.04094 17200.00 10000.00 0.00 0.04274 17400.00 10000.00 0.00 0.04461 17600.00 10000.00 0.00 0.04651 17800.00 10000.00 0.00 0.04839 18000.00 10000.00 0.00 0.05035 18200.00 10000.00 0.00 0.05227 18400.00 10000.00 0.00 0.05437 18600.00 10000.00 0.00 0.05652 18800.00 10000.00 0.00 0.05876 19000.00 10000.00 0.00 0.06107 19200.00 10000.00 0.00 0.06271 19400.00 10000.00 0.00 0.06365 19600.00 10000.00 0.00 0.06415 19800.00 10000.00 0.00 0.06427 20000.00 10000.00 0.00 0.06437 20200.00 10000.00 0.00 0.06437 19600.00 10200.00 0.00 0.06476 19800.00 10200.00 0.00 0.06491 20000.00 10200.00 0.00 0.06504 20200.00 10200.00 0.00 0.06504 19600.00 10400.00 0.00 0.06548 19800.00 10400.00 0.00 0.06566 20000.00 10400.00 0.00 0.06582 20200.00 10400.00 0.00 0.06583 19600.00 10600.00 0.00 0.06633 19800.00 10600.00 0.00 0.06654 20000.00 10600.00 0.00 0.06673 20200.00 10600.00 0.00 0.06674 19600.00 10800.00 0.00 0.06725 19800.00 10800.00 0.00 0.06750 20000.00 10800.00 0.00 0.06772 20200.00 10800.00 0.00 0.06774 19400.00 11000.00 0.00 0.06787 19600.00 11000.00 0.00 0.06845 19800.00 11000.00 0.00 0.06874 20000.00 11000.00 0.00 0.06894 19400.00 11200.00 0.00 0.06899 19600.00 11200.00 0.00 0.06958 19800.00 11200.00 0.00 0.06984 20000.00 11200.00 0.00 0.06996 19400.00 11400.00 0.00 0.07011 19600.00 11400.00 0.00 0.07066 19800.00 11400.00 0.00 0.07087 20000.00 11400.00 0.00 0.07094 19400.00 11600.00 0.00 0.07126 19600.00 11600.00 0.00 0.07176 19800.00 11600.00 0.00 0.07196 20000.00 11600.00 0.00 0.07203 19400.00 11800.00 0.00 0.07248 19600.00 11800.00 0.00 0.07297 19800.00 11800.00 0.00 0.07316 20000.00 11800.00 0.00 0.07323 19400.00 12000.00 0.00 0.07361 19600.00 12000.00 0.00 0.07407 19800.00 12000.00 0.00 0.07424 20000.00 12000.00 0.00 0.07426 19400.00 12200.00 0.00 0.07467 19600.00 12200.00 0.00 0.07511 19800.00 12200.00 0.00 0.07527 19400.00 12400.00 0.00 0.07593 19600.00 12400.00 0.00 0.07638 19800.00 12400.00 0.00 0.07652 19400.00 12600.00 0.00 0.07722 19600.00 12600.00 0.00 0.07764 19800.00 12600.00 0.00 0.07774 19400.00 12800.00 0.00 0.07847 19600.00 12800.00 0.00 0.07886 19800.00 12800.00 0.00 0.07895 19400.00 13000.00 0.00 0.07976 19600.00 13000.00 0.00 0.08020 19800.00 13000.00 0.00 0.08034 20000.00 13000.00 0.00 0.08035 19200.00 13200.00 0.00 0.08037 19400.00 13200.00 0.00 0.08139 19600.00 13200.00 0.00 0.08186 19800.00 13200.00 0.00 0.08202 20000.00 13200.00 0.00 0.08204 19400.00 13400.00 0.00 0.08304 19600.00 13400.00 0.00 0.08350 19800.00 13400.00 0.00 0.08365 19400.00 13600.00 0.00 0.08470 19600.00 13600.00 0.00 0.08514 19800.00 13600.00 0.00 0.08528 19200.00 13800.00 0.00 0.08536 19400.00 13800.00 0.00 0.08640 19600.00 13800.00 0.00 0.08682 19800.00 13800.00 0.00 0.08693 19200.00 14000.00 0.00 0.08705 19400.00 14000.00 0.00 0.08806 19600.00 14000.00 0.00 0.08845 19800.00 14000.00 0.00 0.08854 19200.00 14200.00 0.00 0.08886 19400.00 14200.00 0.00 0.08986 19600.00 14200.00 0.00 0.09026 19800.00 14200.00 0.00 0.09036 19200.00 14400.00 0.00 0.09064 19400.00 14400.00 0.00 0.09168 19600.00 14400.00 0.00 0.09209 19800.00 14400.00 0.00 0.09218 19200.00 14600.00 0.00 0.09257 19400.00 14600.00 0.00 0.09363 19600.00 14600.00 0.00 0.09399 19800.00 14600.00 0.00 0.09401 19200.00 14800.00 0.00 0.09457 19400.00 14800.00 0.00 0.09559 19600.00 14800.00 0.00 0.09586 19200.00 15000.00 0.00 0.09666 19400.00 15000.00 0.00 0.09758 19600.00 15000.00 0.00 0.09776 19200.00 15200.00 0.00 0.09863 19400.00 15200.00 0.00 0.09946 19600.00 15200.00 0.00 0.09957 19200.00 15400.00 0.00 0.10057 19400.00 15400.00 0.00 0.10136 19600.00 15400.00 0.00 0.10144 19200.00 15600.00 0.00 0.10259 19400.00 15600.00 0.00 0.10342 19600.00 15600.00 0.00 0.10350 19200.00 15800.00 0.00 0.10467 19400.00 15800.00 0.00 0.10551 19600.00 15800.00 0.00 0.10553 19200.00 16000.00 0.00 0.10694 19400.00 16000.00 0.00 0.10770 19200.00 16200.00 0.00 0.10902 19400.00 16200.00 0.00 0.10968 19200.00 16400.00 0.00 0.11120 19400.00 16400.00 0.00 0.11179 19200.00 16600.00 0.00 0.11334 19400.00 16600.00 0.00 0.11390 19000.00 16800.00 0.00 0.11397 19200.00 16800.00 0.00 0.11556 19400.00 16800.00 0.00 0.11611 19000.00 17000.00 0.00 0.11634 19200.00 17000.00 0.00 0.11794 19400.00 17000.00 0.00 0.11849 19000.00 17200.00 0.00 0.11880 19200.00 17200.00 0.00 0.12043 19400.00 17200.00 0.00 0.12094 19000.00 17400.00 0.00 0.12156 19200.00 17400.00 0.00 0.12314 19400.00 17400.00 0.00 0.12352 19000.00 17600.00 0.00 0.12402 19200.00 17600.00 0.00 0.12554 19400.00 17600.00 0.00 0.12576 19000.00 17800.00 0.00 0.12674 19200.00 17800.00 0.00 0.12817 19400.00 17800.00 0.00 0.12825 19000.00 18000.00 0.00 0.12948 19200.00 18000.00 0.00 0.13083 19000.00 18200.00 0.00 0.13240 19200.00 18200.00 0.00 0.13361 19000.00 18400.00 0.00 0.13545 19200.00 18400.00 0.00 0.13642 19000.00 18600.00 0.00 0.13844 19200.00 18600.00 0.00 0.13902 18800.00 18800.00 0.00 0.13936 19000.00 18800.00 0.00 0.14134 18800.00 19000.00 0.00 0.14136 19000.00 19000.00 0.00 0.14257 19000.00 19200.00 5.00 0.14268 19000.00 19200.00 10.00 0.14279 19000.00 19200.00 15.00 0.14288 19000.00 19200.00 20.00 0.14296 19000.00 19200.00 25.00 0.14302 19000.00 19200.00 30.00 0.14306 19000.00 19200.00 35.00 0.14308 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18918.50 19188.74 37.09 0.14322 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/081.ctf NX, NY, NZ: 512 512 1 MODE : real