CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/075.mrc Output diagnostic file name Micrographs_0601/075.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/075.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2266.000 8068.000 Mean, RMS : 4301.972 208.0024 TITLE 1: emi2mrc: 075.emi Sun Jan 05 14:37:12 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.10%, Obj 88.09%, Dif 73.67% TITLE 3: X 71.253, Y 321.079, Z -100.481, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.05738 10200.00 10000.00 0.00 0.06011 10400.00 10000.00 0.00 0.06238 10600.00 10000.00 0.00 0.06514 10800.00 10000.00 0.00 0.06811 11000.00 10000.00 0.00 0.07120 11200.00 10000.00 0.00 0.07440 11400.00 10000.00 0.00 0.07769 11600.00 10000.00 0.00 0.08081 11800.00 10000.00 0.00 0.08394 12000.00 10000.00 0.00 0.08734 12200.00 10000.00 0.00 0.09029 12400.00 10000.00 0.00 0.09343 12600.00 10000.00 0.00 0.09640 12800.00 10000.00 0.00 0.09935 13000.00 10000.00 0.00 0.10235 13200.00 10000.00 0.00 0.10439 13400.00 10000.00 0.00 0.10547 13600.00 10000.00 0.00 0.10552 13200.00 10200.00 0.00 0.10664 13400.00 10200.00 0.00 0.10768 13600.00 10200.00 0.00 0.10770 13200.00 10400.00 0.00 0.10905 13400.00 10400.00 0.00 0.11006 13200.00 10600.00 0.00 0.11172 13400.00 10600.00 0.00 0.11272 13000.00 10800.00 0.00 0.11284 13200.00 10800.00 0.00 0.11470 13400.00 10800.00 0.00 0.11563 13000.00 11000.00 0.00 0.11593 13200.00 11000.00 0.00 0.11765 13400.00 11000.00 0.00 0.11843 13200.00 11200.00 0.00 0.12002 13400.00 11200.00 0.00 0.12074 13000.00 11400.00 0.00 0.12126 13200.00 11400.00 0.00 0.12280 13400.00 11400.00 0.00 0.12351 13000.00 11600.00 0.00 0.12389 13200.00 11600.00 0.00 0.12540 13400.00 11600.00 0.00 0.12607 13000.00 11800.00 0.00 0.12666 13200.00 11800.00 0.00 0.12813 13400.00 11800.00 0.00 0.12872 13000.00 12000.00 0.00 0.12951 13200.00 12000.00 0.00 0.13089 13400.00 12000.00 0.00 0.13134 13000.00 12200.00 0.00 0.13207 13200.00 12200.00 0.00 0.13334 13400.00 12200.00 0.00 0.13359 13000.00 12400.00 0.00 0.13490 13200.00 12400.00 0.00 0.13600 13400.00 12400.00 0.00 0.13602 13000.00 12600.00 0.00 0.13735 13200.00 12600.00 0.00 0.13818 13000.00 12800.00 0.00 0.13988 13200.00 12800.00 0.00 0.14020 13000.00 13000.00 0.00 0.14107 13200.00 13000.00 0.00 0.14107 13000.00 13200.00 0.00 0.14172 13200.00 13200.00 0.00 0.14178 13000.00 13400.00 0.00 0.14222 13000.00 13400.00 5.00 0.14223 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13000.00 13382.61 5.00 0.14224 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/075.ctf NX, NY, NZ: 512 512 1 MODE : real