CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/064.mrc Output diagnostic file name Micrographs_0601/064.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/064.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2086.000 8192.000 Mean, RMS : 4508.897 223.1514 TITLE 1: emi2mrc: 064.emi Sun Jan 05 14:24:46 2014 TITLE 2: kV 300, Mag 78000x, Def 21820, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 82.078, Y 322.537, Z -99.328, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00574 10400.00 10000.00 0.00 -0.00568 11600.00 10000.00 0.00 -0.00553 11800.00 10000.00 0.00 -0.00532 12000.00 10000.00 0.00 -0.00510 12200.00 10000.00 0.00 -0.00484 12400.00 10000.00 0.00 -0.00445 12600.00 10000.00 0.00 -0.00395 12800.00 10000.00 0.00 -0.00360 13000.00 10000.00 0.00 -0.00321 13200.00 10000.00 0.00 -0.00275 13400.00 10000.00 0.00 -0.00239 13600.00 10000.00 0.00 -0.00191 13800.00 10000.00 0.00 -0.00133 14000.00 10000.00 0.00 -0.00077 14200.00 10000.00 0.00 -0.00017 14400.00 10000.00 0.00 0.00038 14600.00 10000.00 0.00 0.00088 14800.00 10000.00 0.00 0.00150 15000.00 10000.00 0.00 0.00214 15200.00 10000.00 0.00 0.00287 15400.00 10000.00 0.00 0.00378 15600.00 10000.00 0.00 0.00464 15800.00 10000.00 0.00 0.00556 16000.00 10000.00 0.00 0.00663 16200.00 10000.00 0.00 0.00760 16400.00 10000.00 0.00 0.00863 16600.00 10000.00 0.00 0.00981 16800.00 10000.00 0.00 0.01098 17000.00 10000.00 0.00 0.01210 17200.00 10000.00 0.00 0.01322 17400.00 10000.00 0.00 0.01435 17600.00 10000.00 0.00 0.01546 17800.00 10000.00 0.00 0.01662 18000.00 10000.00 0.00 0.01787 18200.00 10000.00 0.00 0.01904 18400.00 10000.00 0.00 0.02011 18600.00 10000.00 0.00 0.02120 18800.00 10000.00 0.00 0.02253 19000.00 10000.00 0.00 0.02396 19200.00 10000.00 0.00 0.02519 19400.00 10000.00 0.00 0.02641 19600.00 10000.00 0.00 0.02780 19800.00 10000.00 0.00 0.02928 20000.00 10000.00 0.00 0.03082 20200.00 10000.00 0.00 0.03252 20400.00 10000.00 0.00 0.03427 20600.00 10000.00 0.00 0.03601 20800.00 10000.00 0.00 0.03790 21000.00 10000.00 0.00 0.03983 21200.00 10000.00 0.00 0.04163 21400.00 10000.00 0.00 0.04343 21600.00 10000.00 0.00 0.04491 21800.00 10000.00 0.00 0.04580 22000.00 10000.00 0.00 0.04649 22200.00 10000.00 0.00 0.04698 22400.00 10000.00 0.00 0.04718 22600.00 10000.00 0.00 0.04728 22200.00 10200.00 0.00 0.04772 22400.00 10200.00 0.00 0.04789 22600.00 10200.00 0.00 0.04794 22200.00 10400.00 0.00 0.04835 22400.00 10400.00 0.00 0.04847 22600.00 10400.00 0.00 0.04848 22000.00 10600.00 0.00 0.04851 22200.00 10600.00 0.00 0.04894 22400.00 10600.00 0.00 0.04904 22600.00 10600.00 0.00 0.04905 22000.00 10800.00 0.00 0.04919 22200.00 10800.00 0.00 0.04960 22400.00 10800.00 0.00 0.04970 22600.00 10800.00 0.00 0.04971 22000.00 11000.00 0.00 0.05005 22200.00 11000.00 0.00 0.05043 22400.00 11000.00 0.00 0.05053 22000.00 11200.00 0.00 0.05077 22200.00 11200.00 0.00 0.05113 22400.00 11200.00 0.00 0.05122 22000.00 11400.00 0.00 0.05136 22200.00 11400.00 0.00 0.05175 22400.00 11400.00 0.00 0.05188 22000.00 11600.00 0.00 0.05224 22200.00 11600.00 0.00 0.05269 22400.00 11600.00 0.00 0.05283 22000.00 11800.00 0.00 0.05330 22200.00 11800.00 0.00 0.05373 22400.00 11800.00 0.00 0.05381 22000.00 12000.00 0.00 0.05414 22200.00 12000.00 0.00 0.05452 22400.00 12000.00 0.00 0.05455 22000.00 12200.00 0.00 0.05494 22200.00 12200.00 0.00 0.05532 22000.00 12400.00 0.00 0.05588 22200.00 12400.00 0.00 0.05624 22000.00 12600.00 0.00 0.05682 22200.00 12600.00 0.00 0.05715 22000.00 12800.00 0.00 0.05779 22200.00 12800.00 0.00 0.05806 22000.00 13000.00 0.00 0.05872 22200.00 13000.00 0.00 0.05896 21800.00 13200.00 0.00 0.05913 22000.00 13200.00 0.00 0.05977 22200.00 13200.00 0.00 0.06000 21800.00 13400.00 0.00 0.06019 22000.00 13400.00 0.00 0.06080 22200.00 13400.00 0.00 0.06103 21800.00 13600.00 0.00 0.06118 22000.00 13600.00 0.00 0.06178 22200.00 13600.00 0.00 0.06201 21800.00 13800.00 0.00 0.06225 22000.00 13800.00 0.00 0.06286 22200.00 13800.00 0.00 0.06308 21800.00 14000.00 0.00 0.06333 22000.00 14000.00 0.00 0.06394 22200.00 14000.00 0.00 0.06415 21800.00 14200.00 0.00 0.06445 22000.00 14200.00 0.00 0.06506 22200.00 14200.00 0.00 0.06526 21800.00 14400.00 0.00 0.06558 22000.00 14400.00 0.00 0.06618 22200.00 14400.00 0.00 0.06637 21800.00 14600.00 0.00 0.06677 22000.00 14600.00 0.00 0.06738 22200.00 14600.00 0.00 0.06753 21800.00 14800.00 0.00 0.06803 22000.00 14800.00 0.00 0.06860 22200.00 14800.00 0.00 0.06869 21800.00 15000.00 0.00 0.06933 22000.00 15000.00 0.00 0.06984 22200.00 15000.00 0.00 0.06985 21800.00 15200.00 0.00 0.07057 22000.00 15200.00 0.00 0.07103 22200.00 15200.00 0.00 0.07104 21800.00 15400.00 0.00 0.07184 22000.00 15400.00 0.00 0.07228 22200.00 15400.00 0.00 0.07231 21800.00 15600.00 0.00 0.07321 22000.00 15600.00 0.00 0.07363 22200.00 15600.00 0.00 0.07366 21800.00 15800.00 0.00 0.07452 22000.00 15800.00 0.00 0.07493 22200.00 15800.00 0.00 0.07499 21600.00 16000.00 0.00 0.07516 21800.00 16000.00 0.00 0.07605 22000.00 16000.00 0.00 0.07646 22200.00 16000.00 0.00 0.07651 21600.00 16200.00 0.00 0.07681 21800.00 16200.00 0.00 0.07767 22000.00 16200.00 0.00 0.07806 21600.00 16400.00 0.00 0.07854 21800.00 16400.00 0.00 0.07936 22000.00 16400.00 0.00 0.07970 21600.00 16600.00 0.00 0.08019 21800.00 16600.00 0.00 0.08097 22000.00 16600.00 0.00 0.08129 21600.00 16800.00 0.00 0.08169 21800.00 16800.00 0.00 0.08246 22000.00 16800.00 0.00 0.08275 21600.00 17000.00 0.00 0.08323 21800.00 17000.00 0.00 0.08400 22000.00 17000.00 0.00 0.08429 21600.00 17200.00 0.00 0.08479 21800.00 17200.00 0.00 0.08556 22000.00 17200.00 0.00 0.08584 21600.00 17400.00 0.00 0.08643 21800.00 17400.00 0.00 0.08720 22000.00 17400.00 0.00 0.08747 21600.00 17600.00 0.00 0.08810 21800.00 17600.00 0.00 0.08888 22000.00 17600.00 0.00 0.08914 21600.00 17800.00 0.00 0.08987 21800.00 17800.00 0.00 0.09065 22000.00 17800.00 0.00 0.09087 21600.00 18000.00 0.00 0.09161 21800.00 18000.00 0.00 0.09235 22000.00 18000.00 0.00 0.09252 21600.00 18200.00 0.00 0.09341 21800.00 18200.00 0.00 0.09410 22000.00 18200.00 0.00 0.09422 21600.00 18400.00 0.00 0.09524 21800.00 18400.00 0.00 0.09588 22000.00 18400.00 0.00 0.09599 21600.00 18600.00 0.00 0.09713 21800.00 18600.00 0.00 0.09777 22000.00 18600.00 0.00 0.09790 21600.00 18800.00 0.00 0.09926 21800.00 18800.00 0.00 0.09989 22000.00 18800.00 0.00 0.09999 21600.00 19000.00 0.00 0.10147 21800.00 19000.00 0.00 0.10206 22000.00 19000.00 0.00 0.10210 21400.00 19200.00 0.00 0.10227 21600.00 19200.00 0.00 0.10391 21800.00 19200.00 0.00 0.10442 21400.00 19400.00 0.00 0.10462 21600.00 19400.00 0.00 0.10619 21800.00 19400.00 0.00 0.10661 21400.00 19600.00 0.00 0.10699 21600.00 19600.00 0.00 0.10849 21800.00 19600.00 0.00 0.10885 21400.00 19800.00 0.00 0.10946 21600.00 19800.00 0.00 0.11095 21800.00 19800.00 0.00 0.11126 21400.00 20000.00 0.00 0.11205 21600.00 20000.00 0.00 0.11354 21800.00 20000.00 0.00 0.11380 21400.00 20200.00 0.00 0.11484 21600.00 20200.00 0.00 0.11635 21800.00 20200.00 0.00 0.11646 21400.00 20400.00 0.00 0.11773 21600.00 20400.00 0.00 0.11915 21400.00 20600.00 0.00 0.12072 21600.00 20600.00 0.00 0.12197 21400.00 20800.00 0.00 0.12344 21600.00 20800.00 0.00 0.12449 21400.00 21000.00 0.00 0.12640 21600.00 21000.00 0.00 0.12715 21200.00 21200.00 0.00 0.12719 21400.00 21200.00 0.00 0.12887 21600.00 21200.00 0.00 0.12924 21400.00 21400.00 0.00 0.13046 21400.00 21600.00 0.00 0.13072 21400.00 21600.00 5.00 0.13086 21400.00 21600.00 10.00 0.13098 21400.00 21600.00 15.00 0.13110 21400.00 21600.00 20.00 0.13120 21400.00 21600.00 25.00 0.13128 21400.00 21600.00 30.00 0.13133 21400.00 21600.00 35.00 0.13136 21400.00 21600.00 40.00 0.13136 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21376.39 21600.00 37.44 0.13138 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/064.ctf NX, NY, NZ: 512 512 1 MODE : real