CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/058.mrc Output diagnostic file name Micrographs_0601/058.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/058.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2284.000 9038.000 Mean, RMS : 4522.395 222.1709 TITLE 1: emi2mrc: 058.emi Sun Jan 05 14:18:42 2014 TITLE 2: kV 300, Mag 78000x, Def 22970, Spot 4, Int 55.10%, Obj 88.04%, Dif 73.67% TITLE 3: X 87.705, Y 326.757, Z -99.328, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00050 10200.00 10000.00 0.00 -0.00026 10400.00 10000.00 0.00 -0.00003 10600.00 10000.00 0.00 0.00022 10800.00 10000.00 0.00 0.00038 11000.00 10000.00 0.00 0.00055 11200.00 10000.00 0.00 0.00076 11400.00 10000.00 0.00 0.00101 11600.00 10000.00 0.00 0.00127 11800.00 10000.00 0.00 0.00160 12000.00 10000.00 0.00 0.00195 12200.00 10000.00 0.00 0.00211 12400.00 10000.00 0.00 0.00231 12600.00 10000.00 0.00 0.00268 12800.00 10000.00 0.00 0.00304 13000.00 10000.00 0.00 0.00335 13200.00 10000.00 0.00 0.00369 13400.00 10000.00 0.00 0.00410 13600.00 10000.00 0.00 0.00454 13800.00 10000.00 0.00 0.00497 14000.00 10000.00 0.00 0.00545 14200.00 10000.00 0.00 0.00605 14400.00 10000.00 0.00 0.00672 14600.00 10000.00 0.00 0.00743 14800.00 10000.00 0.00 0.00810 15000.00 10000.00 0.00 0.00877 15200.00 10000.00 0.00 0.00949 15400.00 10000.00 0.00 0.01019 15600.00 10000.00 0.00 0.01101 15800.00 10000.00 0.00 0.01196 16000.00 10000.00 0.00 0.01291 16200.00 10000.00 0.00 0.01384 16400.00 10000.00 0.00 0.01466 16600.00 10000.00 0.00 0.01559 16800.00 10000.00 0.00 0.01662 17000.00 10000.00 0.00 0.01775 17200.00 10000.00 0.00 0.01912 17400.00 10000.00 0.00 0.02048 17600.00 10000.00 0.00 0.02184 17800.00 10000.00 0.00 0.02327 18000.00 10000.00 0.00 0.02452 18200.00 10000.00 0.00 0.02566 18400.00 10000.00 0.00 0.02695 18600.00 10000.00 0.00 0.02824 18800.00 10000.00 0.00 0.02939 19000.00 10000.00 0.00 0.03053 19200.00 10000.00 0.00 0.03178 19400.00 10000.00 0.00 0.03319 19600.00 10000.00 0.00 0.03475 19800.00 10000.00 0.00 0.03637 20000.00 10000.00 0.00 0.03805 20200.00 10000.00 0.00 0.03973 20400.00 10000.00 0.00 0.04140 20600.00 10000.00 0.00 0.04314 20800.00 10000.00 0.00 0.04490 21000.00 10000.00 0.00 0.04680 21200.00 10000.00 0.00 0.04880 21400.00 10000.00 0.00 0.05093 21600.00 10000.00 0.00 0.05285 21800.00 10000.00 0.00 0.05392 22000.00 10000.00 0.00 0.05453 22200.00 10000.00 0.00 0.05484 22400.00 10000.00 0.00 0.05492 22000.00 10200.00 0.00 0.05496 22200.00 10200.00 0.00 0.05527 22400.00 10200.00 0.00 0.05536 22000.00 10400.00 0.00 0.05545 22200.00 10400.00 0.00 0.05577 22400.00 10400.00 0.00 0.05585 22000.00 10600.00 0.00 0.05590 22200.00 10600.00 0.00 0.05620 22400.00 10600.00 0.00 0.05627 22000.00 10800.00 0.00 0.05628 22200.00 10800.00 0.00 0.05657 22400.00 10800.00 0.00 0.05665 22000.00 11000.00 0.00 0.05688 22200.00 11000.00 0.00 0.05717 22400.00 11000.00 0.00 0.05726 22000.00 11200.00 0.00 0.05750 22200.00 11200.00 0.00 0.05777 22400.00 11200.00 0.00 0.05787 22000.00 11400.00 0.00 0.05821 22200.00 11400.00 0.00 0.05847 22400.00 11400.00 0.00 0.05857 22000.00 11600.00 0.00 0.05875 22200.00 11600.00 0.00 0.05902 22400.00 11600.00 0.00 0.05914 22000.00 11800.00 0.00 0.05928 22200.00 11800.00 0.00 0.05958 22400.00 11800.00 0.00 0.05972 22000.00 12000.00 0.00 0.05999 22200.00 12000.00 0.00 0.06032 22400.00 12000.00 0.00 0.06047 22000.00 12200.00 0.00 0.06077 22200.00 12200.00 0.00 0.06113 22400.00 12200.00 0.00 0.06126 22000.00 12400.00 0.00 0.06176 22200.00 12400.00 0.00 0.06211 22400.00 12400.00 0.00 0.06220 22000.00 12600.00 0.00 0.06272 22200.00 12600.00 0.00 0.06304 22400.00 12600.00 0.00 0.06310 21800.00 12800.00 0.00 0.06311 22000.00 12800.00 0.00 0.06374 22200.00 12800.00 0.00 0.06403 22400.00 12800.00 0.00 0.06407 21800.00 13000.00 0.00 0.06411 22000.00 13000.00 0.00 0.06473 22200.00 13000.00 0.00 0.06499 21800.00 13200.00 0.00 0.06501 22000.00 13200.00 0.00 0.06563 22200.00 13200.00 0.00 0.06586 21800.00 13400.00 0.00 0.06591 22000.00 13400.00 0.00 0.06648 22200.00 13400.00 0.00 0.06663 21800.00 13600.00 0.00 0.06684 22000.00 13600.00 0.00 0.06732 22200.00 13600.00 0.00 0.06741 21800.00 13800.00 0.00 0.06783 22000.00 13800.00 0.00 0.06828 22200.00 13800.00 0.00 0.06838 21800.00 14000.00 0.00 0.06890 22000.00 14000.00 0.00 0.06933 22200.00 14000.00 0.00 0.06943 21800.00 14200.00 0.00 0.07004 22000.00 14200.00 0.00 0.07042 22200.00 14200.00 0.00 0.07050 21800.00 14400.00 0.00 0.07107 22000.00 14400.00 0.00 0.07144 22200.00 14400.00 0.00 0.07154 21800.00 14600.00 0.00 0.07222 22000.00 14600.00 0.00 0.07262 22200.00 14600.00 0.00 0.07275 21800.00 14800.00 0.00 0.07349 22000.00 14800.00 0.00 0.07391 22200.00 14800.00 0.00 0.07405 21800.00 15000.00 0.00 0.07484 22000.00 15000.00 0.00 0.07529 22200.00 15000.00 0.00 0.07540 21800.00 15200.00 0.00 0.07626 22000.00 15200.00 0.00 0.07669 22200.00 15200.00 0.00 0.07675 21800.00 15400.00 0.00 0.07757 22000.00 15400.00 0.00 0.07796 22200.00 15400.00 0.00 0.07797 21800.00 15600.00 0.00 0.07885 22000.00 15600.00 0.00 0.07926 22200.00 15600.00 0.00 0.07928 21800.00 15800.00 0.00 0.08019 22000.00 15800.00 0.00 0.08064 22200.00 15800.00 0.00 0.08064 21600.00 16000.00 0.00 0.08065 21800.00 16000.00 0.00 0.08165 22000.00 16000.00 0.00 0.08207 21600.00 16200.00 0.00 0.08209 21800.00 16200.00 0.00 0.08309 22000.00 16200.00 0.00 0.08350 21600.00 16400.00 0.00 0.08366 21800.00 16400.00 0.00 0.08470 22000.00 16400.00 0.00 0.08513 21600.00 16600.00 0.00 0.08537 21800.00 16600.00 0.00 0.08647 22000.00 16600.00 0.00 0.08690 21600.00 16800.00 0.00 0.08714 21800.00 16800.00 0.00 0.08826 22000.00 16800.00 0.00 0.08863 21600.00 17000.00 0.00 0.08884 21800.00 17000.00 0.00 0.08997 22000.00 17000.00 0.00 0.09028 21600.00 17200.00 0.00 0.09061 21800.00 17200.00 0.00 0.09174 22000.00 17200.00 0.00 0.09199 21600.00 17400.00 0.00 0.09256 21800.00 17400.00 0.00 0.09365 22000.00 17400.00 0.00 0.09381 21600.00 17600.00 0.00 0.09435 21800.00 17600.00 0.00 0.09538 22000.00 17600.00 0.00 0.09547 21600.00 17800.00 0.00 0.09625 21800.00 17800.00 0.00 0.09721 22000.00 17800.00 0.00 0.09725 21600.00 18000.00 0.00 0.09821 21800.00 18000.00 0.00 0.09911 21600.00 18200.00 0.00 0.10029 21800.00 18200.00 0.00 0.10114 21600.00 18400.00 0.00 0.10235 21800.00 18400.00 0.00 0.10312 21600.00 18600.00 0.00 0.10425 21800.00 18600.00 0.00 0.10496 21600.00 18800.00 0.00 0.10625 21800.00 18800.00 0.00 0.10695 21600.00 19000.00 0.00 0.10826 21800.00 19000.00 0.00 0.10897 21600.00 19200.00 0.00 0.11045 21800.00 19200.00 0.00 0.11115 21600.00 19400.00 0.00 0.11266 21800.00 19400.00 0.00 0.11332 21600.00 19600.00 0.00 0.11507 21800.00 19600.00 0.00 0.11565 21600.00 19800.00 0.00 0.11744 21800.00 19800.00 0.00 0.11792 21600.00 20000.00 0.00 0.11982 21800.00 20000.00 0.00 0.12021 21600.00 20200.00 0.00 0.12233 21800.00 20200.00 0.00 0.12257 21600.00 20400.00 0.00 0.12470 21800.00 20400.00 0.00 0.12477 21400.00 20600.00 0.00 0.12517 21600.00 20600.00 0.00 0.12726 21400.00 20800.00 0.00 0.12777 21600.00 20800.00 0.00 0.12971 21400.00 21000.00 0.00 0.13055 21600.00 21000.00 0.00 0.13227 21400.00 21200.00 0.00 0.13302 21600.00 21200.00 0.00 0.13448 21400.00 21400.00 0.00 0.13539 21600.00 21400.00 0.00 0.13637 21400.00 21600.00 0.00 0.13681 21600.00 21600.00 0.00 0.13684 21400.00 21600.00 5.00 0.13696 21400.00 21600.00 10.00 0.13709 21400.00 21600.00 15.00 0.13721 21400.00 21600.00 20.00 0.13731 21400.00 21600.00 25.00 0.13738 21400.00 21600.00 30.00 0.13744 21400.00 21600.00 35.00 0.13746 21400.00 21600.00 40.00 0.13747 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21456.22 21626.99 35.14 0.13768 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/058.ctf NX, NY, NZ: 512 512 1 MODE : real