CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/054.mrc Output diagnostic file name Micrographs_0601/054.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/054.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2264.000 8784.000 Mean, RMS : 4502.065 223.9430 TITLE 1: emi2mrc: 054.emi Sun Jan 05 14:13:51 2014 TITLE 2: kV 300, Mag 78000x, Def 23630, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 79.964, Y 330.351, Z -99.328, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01825 15200.00 10000.00 0.00 -0.01814 15400.00 10000.00 0.00 -0.01769 15600.00 10000.00 0.00 -0.01743 15800.00 10000.00 0.00 -0.01704 16000.00 10000.00 0.00 -0.01645 16200.00 10000.00 0.00 -0.01590 16400.00 10000.00 0.00 -0.01530 16600.00 10000.00 0.00 -0.01473 16800.00 10000.00 0.00 -0.01415 17000.00 10000.00 0.00 -0.01358 17200.00 10000.00 0.00 -0.01307 17400.00 10000.00 0.00 -0.01252 17600.00 10000.00 0.00 -0.01195 17800.00 10000.00 0.00 -0.01130 18000.00 10000.00 0.00 -0.01040 18200.00 10000.00 0.00 -0.00944 18400.00 10000.00 0.00 -0.00851 18600.00 10000.00 0.00 -0.00751 18800.00 10000.00 0.00 -0.00657 19000.00 10000.00 0.00 -0.00574 19200.00 10000.00 0.00 -0.00492 19400.00 10000.00 0.00 -0.00396 19600.00 10000.00 0.00 -0.00294 19800.00 10000.00 0.00 -0.00199 20000.00 10000.00 0.00 -0.00097 20200.00 10000.00 0.00 0.00007 20400.00 10000.00 0.00 0.00095 20600.00 10000.00 0.00 0.00172 20800.00 10000.00 0.00 0.00263 21000.00 10000.00 0.00 0.00365 21200.00 10000.00 0.00 0.00467 21400.00 10000.00 0.00 0.00581 21600.00 10000.00 0.00 0.00706 21800.00 10000.00 0.00 0.00837 22000.00 10000.00 0.00 0.00972 22200.00 10000.00 0.00 0.01100 22400.00 10000.00 0.00 0.01222 22600.00 10000.00 0.00 0.01361 22800.00 10000.00 0.00 0.01516 23000.00 10000.00 0.00 0.01668 23200.00 10000.00 0.00 0.01816 23400.00 10000.00 0.00 0.01971 23600.00 10000.00 0.00 0.02127 23800.00 10000.00 0.00 0.02284 24000.00 10000.00 0.00 0.02434 24200.00 10000.00 0.00 0.02570 24400.00 10000.00 0.00 0.02673 24600.00 10000.00 0.00 0.02730 24800.00 10000.00 0.00 0.02766 25000.00 10000.00 0.00 0.02787 25200.00 10000.00 0.00 0.02799 25400.00 10000.00 0.00 0.02809 25000.00 10200.00 0.00 0.02830 25200.00 10200.00 0.00 0.02842 25400.00 10200.00 0.00 0.02850 24800.00 10400.00 0.00 0.02861 25000.00 10400.00 0.00 0.02884 25200.00 10400.00 0.00 0.02894 25400.00 10400.00 0.00 0.02900 24800.00 10600.00 0.00 0.02921 25000.00 10600.00 0.00 0.02940 25200.00 10600.00 0.00 0.02946 25400.00 10600.00 0.00 0.02948 24800.00 10800.00 0.00 0.02965 25000.00 10800.00 0.00 0.02980 25200.00 10800.00 0.00 0.02982 25400.00 10800.00 0.00 0.02982 24800.00 11000.00 0.00 0.03008 25000.00 11000.00 0.00 0.03019 25200.00 11000.00 0.00 0.03021 24800.00 11200.00 0.00 0.03048 25000.00 11200.00 0.00 0.03057 25200.00 11200.00 0.00 0.03059 25400.00 11200.00 0.00 0.03060 24800.00 11400.00 0.00 0.03075 25000.00 11400.00 0.00 0.03086 25200.00 11400.00 0.00 0.03090 25400.00 11400.00 0.00 0.03092 24800.00 11600.00 0.00 0.03107 25000.00 11600.00 0.00 0.03119 25200.00 11600.00 0.00 0.03123 25400.00 11600.00 0.00 0.03124 24800.00 11800.00 0.00 0.03146 25000.00 11800.00 0.00 0.03156 25200.00 11800.00 0.00 0.03158 25400.00 11800.00 0.00 0.03159 24800.00 12000.00 0.00 0.03188 25000.00 12000.00 0.00 0.03197 25200.00 12000.00 0.00 0.03200 25400.00 12000.00 0.00 0.03204 24800.00 12200.00 0.00 0.03237 25000.00 12200.00 0.00 0.03246 25200.00 12200.00 0.00 0.03251 25400.00 12200.00 0.00 0.03258 24800.00 12400.00 0.00 0.03285 25000.00 12400.00 0.00 0.03294 25200.00 12400.00 0.00 0.03300 25400.00 12400.00 0.00 0.03307 24800.00 12600.00 0.00 0.03337 25000.00 12600.00 0.00 0.03345 25200.00 12600.00 0.00 0.03352 25400.00 12600.00 0.00 0.03356 24600.00 12800.00 0.00 0.03367 24800.00 12800.00 0.00 0.03398 25000.00 12800.00 0.00 0.03404 25200.00 12800.00 0.00 0.03410 24600.00 13000.00 0.00 0.03431 24800.00 13000.00 0.00 0.03459 25000.00 13000.00 0.00 0.03464 25200.00 13000.00 0.00 0.03467 24600.00 13200.00 0.00 0.03495 24800.00 13200.00 0.00 0.03519 25000.00 13200.00 0.00 0.03522 25200.00 13200.00 0.00 0.03524 24600.00 13400.00 0.00 0.03548 24800.00 13400.00 0.00 0.03569 25000.00 13400.00 0.00 0.03571 25200.00 13400.00 0.00 0.03573 24600.00 13600.00 0.00 0.03596 24800.00 13600.00 0.00 0.03616 25000.00 13600.00 0.00 0.03620 25200.00 13600.00 0.00 0.03624 24600.00 13800.00 0.00 0.03641 24800.00 13800.00 0.00 0.03662 25000.00 13800.00 0.00 0.03669 25200.00 13800.00 0.00 0.03676 24600.00 14000.00 0.00 0.03689 24800.00 14000.00 0.00 0.03714 25000.00 14000.00 0.00 0.03724 25200.00 14000.00 0.00 0.03732 24600.00 14200.00 0.00 0.03753 24800.00 14200.00 0.00 0.03782 25000.00 14200.00 0.00 0.03793 25200.00 14200.00 0.00 0.03798 24600.00 14400.00 0.00 0.03826 24800.00 14400.00 0.00 0.03854 25000.00 14400.00 0.00 0.03863 25200.00 14400.00 0.00 0.03867 24600.00 14600.00 0.00 0.03910 24800.00 14600.00 0.00 0.03937 25000.00 14600.00 0.00 0.03945 25200.00 14600.00 0.00 0.03947 24600.00 14800.00 0.00 0.03998 24800.00 14800.00 0.00 0.04022 25000.00 14800.00 0.00 0.04028 24400.00 15000.00 0.00 0.04028 24600.00 15000.00 0.00 0.04089 24800.00 15000.00 0.00 0.04107 25000.00 15000.00 0.00 0.04110 24400.00 15200.00 0.00 0.04121 24600.00 15200.00 0.00 0.04175 24800.00 15200.00 0.00 0.04188 24400.00 15400.00 0.00 0.04212 24600.00 15400.00 0.00 0.04258 24800.00 15400.00 0.00 0.04265 24400.00 15600.00 0.00 0.04293 24600.00 15600.00 0.00 0.04333 24800.00 15600.00 0.00 0.04339 24400.00 15800.00 0.00 0.04376 24600.00 15800.00 0.00 0.04418 24800.00 15800.00 0.00 0.04430 24400.00 16000.00 0.00 0.04491 24600.00 16000.00 0.00 0.04536 24800.00 16000.00 0.00 0.04547 24400.00 16200.00 0.00 0.04597 24600.00 16200.00 0.00 0.04639 24800.00 16200.00 0.00 0.04647 24400.00 16400.00 0.00 0.04703 24600.00 16400.00 0.00 0.04741 24800.00 16400.00 0.00 0.04750 24400.00 16600.00 0.00 0.04813 24600.00 16600.00 0.00 0.04848 24800.00 16600.00 0.00 0.04858 24400.00 16800.00 0.00 0.04921 24600.00 16800.00 0.00 0.04955 24800.00 16800.00 0.00 0.04964 24400.00 17000.00 0.00 0.05042 24600.00 17000.00 0.00 0.05074 24800.00 17000.00 0.00 0.05081 24400.00 17200.00 0.00 0.05150 24600.00 17200.00 0.00 0.05181 24800.00 17200.00 0.00 0.05190 24400.00 17400.00 0.00 0.05267 24600.00 17400.00 0.00 0.05304 24800.00 17400.00 0.00 0.05319 24200.00 17600.00 0.00 0.05322 24400.00 17600.00 0.00 0.05414 24600.00 17600.00 0.00 0.05456 24800.00 17600.00 0.00 0.05471 24200.00 17800.00 0.00 0.05476 24400.00 17800.00 0.00 0.05570 24600.00 17800.00 0.00 0.05612 24800.00 17800.00 0.00 0.05623 24200.00 18000.00 0.00 0.05624 24400.00 18000.00 0.00 0.05720 24600.00 18000.00 0.00 0.05760 24800.00 18000.00 0.00 0.05771 24200.00 18200.00 0.00 0.05777 24400.00 18200.00 0.00 0.05872 24600.00 18200.00 0.00 0.05912 24800.00 18200.00 0.00 0.05923 24200.00 18400.00 0.00 0.05927 24400.00 18400.00 0.00 0.06022 24600.00 18400.00 0.00 0.06060 24800.00 18400.00 0.00 0.06068 24200.00 18600.00 0.00 0.06075 24400.00 18600.00 0.00 0.06170 24600.00 18600.00 0.00 0.06205 24800.00 18600.00 0.00 0.06209 24200.00 18800.00 0.00 0.06229 24400.00 18800.00 0.00 0.06323 24600.00 18800.00 0.00 0.06350 24800.00 18800.00 0.00 0.06351 24200.00 19000.00 0.00 0.06372 24400.00 19000.00 0.00 0.06460 24600.00 19000.00 0.00 0.06482 24200.00 19200.00 0.00 0.06516 24400.00 19200.00 0.00 0.06602 24600.00 19200.00 0.00 0.06625 24800.00 19200.00 0.00 0.06628 24200.00 19400.00 0.00 0.06675 24400.00 19400.00 0.00 0.06763 24600.00 19400.00 0.00 0.06788 24800.00 19400.00 0.00 0.06794 24200.00 19600.00 0.00 0.06846 24400.00 19600.00 0.00 0.06934 24600.00 19600.00 0.00 0.06960 24800.00 19600.00 0.00 0.06964 24200.00 19800.00 0.00 0.07011 24400.00 19800.00 0.00 0.07095 24600.00 19800.00 0.00 0.07117 24200.00 20000.00 0.00 0.07167 24400.00 20000.00 0.00 0.07248 24600.00 20000.00 0.00 0.07269 24200.00 20200.00 0.00 0.07319 24400.00 20200.00 0.00 0.07402 24600.00 20200.00 0.00 0.07424 24200.00 20400.00 0.00 0.07483 24400.00 20400.00 0.00 0.07570 24600.00 20400.00 0.00 0.07591 24200.00 20600.00 0.00 0.07672 24400.00 20600.00 0.00 0.07757 24600.00 20600.00 0.00 0.07775 24200.00 20800.00 0.00 0.07855 24400.00 20800.00 0.00 0.07937 24600.00 20800.00 0.00 0.07952 24200.00 21000.00 0.00 0.08038 24400.00 21000.00 0.00 0.08118 24600.00 21000.00 0.00 0.08130 24200.00 21200.00 0.00 0.08212 24400.00 21200.00 0.00 0.08290 24600.00 21200.00 0.00 0.08297 24200.00 21400.00 0.00 0.08408 24400.00 21400.00 0.00 0.08485 24600.00 21400.00 0.00 0.08492 24200.00 21600.00 0.00 0.08630 24400.00 21600.00 0.00 0.08710 24600.00 21600.00 0.00 0.08717 24200.00 21800.00 0.00 0.08849 24400.00 21800.00 0.00 0.08930 24600.00 21800.00 0.00 0.08932 24200.00 22000.00 0.00 0.09083 24400.00 22000.00 0.00 0.09161 24200.00 22200.00 0.00 0.09328 24400.00 22200.00 0.00 0.09398 24000.00 22400.00 0.00 0.09414 24200.00 22400.00 0.00 0.09579 24400.00 22400.00 0.00 0.09641 24000.00 22600.00 0.00 0.09659 24200.00 22600.00 0.00 0.09823 24400.00 22600.00 0.00 0.09878 24000.00 22800.00 0.00 0.09939 24200.00 22800.00 0.00 0.10099 24400.00 22800.00 0.00 0.10146 24000.00 23000.00 0.00 0.10237 24200.00 23000.00 0.00 0.10391 24400.00 23000.00 0.00 0.10424 24000.00 23200.00 0.00 0.10530 24200.00 23200.00 0.00 0.10675 24400.00 23200.00 0.00 0.10690 24000.00 23400.00 0.00 0.10817 24200.00 23400.00 0.00 0.10947 24000.00 23600.00 0.00 0.11084 24200.00 23600.00 0.00 0.11191 24000.00 23800.00 0.00 0.11362 24200.00 23800.00 0.00 0.11433 24000.00 24000.00 0.00 0.11557 24200.00 24000.00 0.00 0.11592 24000.00 24200.00 0.00 0.11641 24000.00 24200.00 5.00 0.11659 24000.00 24200.00 10.00 0.11676 24000.00 24200.00 15.00 0.11690 24000.00 24200.00 20.00 0.11702 24000.00 24200.00 25.00 0.11710 24000.00 24200.00 30.00 0.11716 24000.00 24200.00 35.00 0.11718 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23975.64 24307.29 36.44 0.11747 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/054.ctf NX, NY, NZ: 512 512 1 MODE : real