CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/053.mrc Output diagnostic file name Micrographs_0601/053.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/053.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2556.000 8018.000 Mean, RMS : 4504.651 224.5470 TITLE 1: emi2mrc: 053.emi Sun Jan 05 14:12:53 2014 TITLE 2: kV 300, Mag 78000x, Def 23630, Spot 4, Int 55.10%, Obj 88.06%, Dif 73.67% TITLE 3: X 82.591, Y 330.203, Z -99.328, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01239 10400.00 10000.00 0.00 -0.01234 10600.00 10000.00 0.00 -0.01218 10800.00 10000.00 0.00 -0.01211 12600.00 10000.00 0.00 -0.01210 12800.00 10000.00 0.00 -0.01197 13000.00 10000.00 0.00 -0.01165 13200.00 10000.00 0.00 -0.01121 13400.00 10000.00 0.00 -0.01076 13600.00 10000.00 0.00 -0.01030 13800.00 10000.00 0.00 -0.00997 14000.00 10000.00 0.00 -0.00962 14200.00 10000.00 0.00 -0.00924 14400.00 10000.00 0.00 -0.00890 14600.00 10000.00 0.00 -0.00840 14800.00 10000.00 0.00 -0.00778 15000.00 10000.00 0.00 -0.00720 15200.00 10000.00 0.00 -0.00659 15400.00 10000.00 0.00 -0.00591 15600.00 10000.00 0.00 -0.00517 15800.00 10000.00 0.00 -0.00429 16000.00 10000.00 0.00 -0.00347 16200.00 10000.00 0.00 -0.00273 16400.00 10000.00 0.00 -0.00193 16600.00 10000.00 0.00 -0.00101 16800.00 10000.00 0.00 0.00007 17000.00 10000.00 0.00 0.00119 17200.00 10000.00 0.00 0.00232 17400.00 10000.00 0.00 0.00344 17600.00 10000.00 0.00 0.00458 17800.00 10000.00 0.00 0.00571 18000.00 10000.00 0.00 0.00690 18200.00 10000.00 0.00 0.00817 18400.00 10000.00 0.00 0.00935 18600.00 10000.00 0.00 0.01045 18800.00 10000.00 0.00 0.01160 19000.00 10000.00 0.00 0.01279 19200.00 10000.00 0.00 0.01401 19400.00 10000.00 0.00 0.01532 19600.00 10000.00 0.00 0.01659 19800.00 10000.00 0.00 0.01770 20000.00 10000.00 0.00 0.01883 20200.00 10000.00 0.00 0.02008 20400.00 10000.00 0.00 0.02143 20600.00 10000.00 0.00 0.02279 20800.00 10000.00 0.00 0.02433 21000.00 10000.00 0.00 0.02618 21200.00 10000.00 0.00 0.02804 21400.00 10000.00 0.00 0.02980 21600.00 10000.00 0.00 0.03164 21800.00 10000.00 0.00 0.03360 22000.00 10000.00 0.00 0.03558 22200.00 10000.00 0.00 0.03754 22400.00 10000.00 0.00 0.03924 22600.00 10000.00 0.00 0.04034 22800.00 10000.00 0.00 0.04112 23000.00 10000.00 0.00 0.04159 23200.00 10000.00 0.00 0.04161 23000.00 10200.00 0.00 0.04193 23000.00 10400.00 0.00 0.04225 22800.00 10600.00 0.00 0.04228 23000.00 10600.00 0.00 0.04255 22800.00 10800.00 0.00 0.04264 23000.00 10800.00 0.00 0.04290 23200.00 10800.00 0.00 0.04295 22800.00 11000.00 0.00 0.04313 23000.00 11000.00 0.00 0.04345 23200.00 11000.00 0.00 0.04358 22800.00 11200.00 0.00 0.04369 23000.00 11200.00 0.00 0.04408 23200.00 11200.00 0.00 0.04421 22800.00 11400.00 0.00 0.04433 23000.00 11400.00 0.00 0.04475 23200.00 11400.00 0.00 0.04484 22800.00 11600.00 0.00 0.04490 23000.00 11600.00 0.00 0.04533 23200.00 11600.00 0.00 0.04540 22800.00 11800.00 0.00 0.04559 23000.00 11800.00 0.00 0.04605 23200.00 11800.00 0.00 0.04610 22800.00 12000.00 0.00 0.04639 23000.00 12000.00 0.00 0.04685 23200.00 12000.00 0.00 0.04690 22800.00 12200.00 0.00 0.04713 23000.00 12200.00 0.00 0.04760 23200.00 12200.00 0.00 0.04765 22800.00 12400.00 0.00 0.04799 23000.00 12400.00 0.00 0.04845 23200.00 12400.00 0.00 0.04849 22800.00 12600.00 0.00 0.04880 23000.00 12600.00 0.00 0.04923 23200.00 12600.00 0.00 0.04925 22800.00 12800.00 0.00 0.04953 23000.00 12800.00 0.00 0.04993 23200.00 12800.00 0.00 0.04993 22800.00 13000.00 0.00 0.05021 23000.00 13000.00 0.00 0.05059 23200.00 13000.00 0.00 0.05059 22800.00 13200.00 0.00 0.05089 23000.00 13200.00 0.00 0.05128 23200.00 13200.00 0.00 0.05129 22800.00 13400.00 0.00 0.05170 23000.00 13400.00 0.00 0.05209 23200.00 13400.00 0.00 0.05210 22800.00 13600.00 0.00 0.05254 23000.00 13600.00 0.00 0.05290 22800.00 13800.00 0.00 0.05322 23000.00 13800.00 0.00 0.05357 22800.00 14000.00 0.00 0.05391 23000.00 14000.00 0.00 0.05429 22800.00 14200.00 0.00 0.05483 23000.00 14200.00 0.00 0.05520 22800.00 14400.00 0.00 0.05575 23000.00 14400.00 0.00 0.05610 22800.00 14600.00 0.00 0.05679 23000.00 14600.00 0.00 0.05713 22600.00 14800.00 0.00 0.05726 22800.00 14800.00 0.00 0.05795 23000.00 14800.00 0.00 0.05830 22600.00 15000.00 0.00 0.05844 22800.00 15000.00 0.00 0.05916 23000.00 15000.00 0.00 0.05956 23200.00 15000.00 0.00 0.05957 22600.00 15200.00 0.00 0.05971 22800.00 15200.00 0.00 0.06047 23000.00 15200.00 0.00 0.06086 22600.00 15400.00 0.00 0.06102 22800.00 15400.00 0.00 0.06175 23000.00 15400.00 0.00 0.06210 22600.00 15600.00 0.00 0.06228 22800.00 15600.00 0.00 0.06296 23000.00 15600.00 0.00 0.06328 22600.00 15800.00 0.00 0.06348 22800.00 15800.00 0.00 0.06416 23000.00 15800.00 0.00 0.06450 22600.00 16000.00 0.00 0.06473 22800.00 16000.00 0.00 0.06544 23000.00 16000.00 0.00 0.06577 22600.00 16200.00 0.00 0.06609 22800.00 16200.00 0.00 0.06679 23000.00 16200.00 0.00 0.06710 22600.00 16400.00 0.00 0.06765 22800.00 16400.00 0.00 0.06832 23000.00 16400.00 0.00 0.06862 22600.00 16600.00 0.00 0.06925 22800.00 16600.00 0.00 0.06992 23000.00 16600.00 0.00 0.07019 22600.00 16800.00 0.00 0.07087 22800.00 16800.00 0.00 0.07151 23000.00 16800.00 0.00 0.07172 22600.00 17000.00 0.00 0.07244 22800.00 17000.00 0.00 0.07304 23000.00 17000.00 0.00 0.07319 22600.00 17200.00 0.00 0.07402 22800.00 17200.00 0.00 0.07459 23000.00 17200.00 0.00 0.07471 22600.00 17400.00 0.00 0.07580 22800.00 17400.00 0.00 0.07635 23000.00 17400.00 0.00 0.07648 22600.00 17600.00 0.00 0.07758 22800.00 17600.00 0.00 0.07814 23000.00 17600.00 0.00 0.07829 22600.00 17800.00 0.00 0.07945 22800.00 17800.00 0.00 0.08001 23000.00 17800.00 0.00 0.08015 22600.00 18000.00 0.00 0.08127 22800.00 18000.00 0.00 0.08179 23000.00 18000.00 0.00 0.08186 22600.00 18200.00 0.00 0.08304 22800.00 18200.00 0.00 0.08351 22400.00 18400.00 0.00 0.08370 22600.00 18400.00 0.00 0.08490 22800.00 18400.00 0.00 0.08530 22400.00 18600.00 0.00 0.08567 22600.00 18600.00 0.00 0.08682 22800.00 18600.00 0.00 0.08713 22400.00 18800.00 0.00 0.08759 22600.00 18800.00 0.00 0.08866 22800.00 18800.00 0.00 0.08890 22400.00 19000.00 0.00 0.08934 22600.00 19000.00 0.00 0.09038 22800.00 19000.00 0.00 0.09057 22400.00 19200.00 0.00 0.09132 22600.00 19200.00 0.00 0.09234 22800.00 19200.00 0.00 0.09248 22400.00 19400.00 0.00 0.09331 22600.00 19400.00 0.00 0.09429 22800.00 19400.00 0.00 0.09435 22400.00 19600.00 0.00 0.09545 22600.00 19600.00 0.00 0.09638 22400.00 19800.00 0.00 0.09781 22600.00 19800.00 0.00 0.09866 22400.00 20000.00 0.00 0.10010 22600.00 20000.00 0.00 0.10086 22400.00 20200.00 0.00 0.10235 22600.00 20200.00 0.00 0.10304 22400.00 20400.00 0.00 0.10473 22600.00 20400.00 0.00 0.10535 22200.00 20600.00 0.00 0.10535 22400.00 20600.00 0.00 0.10732 22600.00 20600.00 0.00 0.10783 22200.00 20800.00 0.00 0.10802 22400.00 20800.00 0.00 0.10992 22600.00 20800.00 0.00 0.11033 22200.00 21000.00 0.00 0.11085 22400.00 21000.00 0.00 0.11269 22600.00 21000.00 0.00 0.11303 22200.00 21200.00 0.00 0.11360 22400.00 21200.00 0.00 0.11540 22600.00 21200.00 0.00 0.11561 22200.00 21400.00 0.00 0.11647 22400.00 21400.00 0.00 0.11822 22600.00 21400.00 0.00 0.11825 22200.00 21600.00 0.00 0.11929 22400.00 21600.00 0.00 0.12093 22200.00 21800.00 0.00 0.12190 22400.00 21800.00 0.00 0.12327 22200.00 22000.00 0.00 0.12446 22400.00 22000.00 0.00 0.12539 22200.00 22200.00 0.00 0.12614 22400.00 22200.00 0.00 0.12662 22200.00 22400.00 0.00 0.12690 22200.00 22400.00 5.00 0.12708 22200.00 22400.00 10.00 0.12725 22200.00 22400.00 15.00 0.12740 22200.00 22400.00 20.00 0.12753 22200.00 22400.00 25.00 0.12764 22200.00 22400.00 30.00 0.12773 22200.00 22400.00 35.00 0.12778 22200.00 22400.00 40.00 0.12780 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22208.79 22476.96 40.00 0.12803 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/053.ctf NX, NY, NZ: 512 512 1 MODE : real