CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/050.mrc Output diagnostic file name Micrographs_0601/050.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/050.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2402.000 8810.000 Mean, RMS : 4510.515 226.6697 TITLE 1: emi2mrc: 050.emi Sun Jan 05 14:09:39 2014 TITLE 2: kV 300, Mag 78000x, Def 23790, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 91.383, Y 334.282, Z -99.328, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00803 10400.00 10000.00 0.00 -0.00803 14800.00 10000.00 0.00 -0.00778 15000.00 10000.00 0.00 -0.00761 15200.00 10000.00 0.00 -0.00735 15400.00 10000.00 0.00 -0.00698 15600.00 10000.00 0.00 -0.00669 15800.00 10000.00 0.00 -0.00645 16000.00 10000.00 0.00 -0.00606 16200.00 10000.00 0.00 -0.00536 16400.00 10000.00 0.00 -0.00471 16600.00 10000.00 0.00 -0.00427 16800.00 10000.00 0.00 -0.00388 17000.00 10000.00 0.00 -0.00343 17200.00 10000.00 0.00 -0.00280 17400.00 10000.00 0.00 -0.00196 17600.00 10000.00 0.00 -0.00114 17800.00 10000.00 0.00 -0.00052 18000.00 10000.00 0.00 0.00009 18200.00 10000.00 0.00 0.00083 18400.00 10000.00 0.00 0.00161 18600.00 10000.00 0.00 0.00231 18800.00 10000.00 0.00 0.00299 19000.00 10000.00 0.00 0.00384 19200.00 10000.00 0.00 0.00485 19400.00 10000.00 0.00 0.00588 19600.00 10000.00 0.00 0.00686 19800.00 10000.00 0.00 0.00767 20000.00 10000.00 0.00 0.00841 20200.00 10000.00 0.00 0.00942 20400.00 10000.00 0.00 0.01051 20600.00 10000.00 0.00 0.01149 20800.00 10000.00 0.00 0.01240 21000.00 10000.00 0.00 0.01323 21200.00 10000.00 0.00 0.01424 21400.00 10000.00 0.00 0.01541 21600.00 10000.00 0.00 0.01661 21800.00 10000.00 0.00 0.01784 22000.00 10000.00 0.00 0.01898 22200.00 10000.00 0.00 0.02025 22400.00 10000.00 0.00 0.02166 22600.00 10000.00 0.00 0.02297 22800.00 10000.00 0.00 0.02425 23000.00 10000.00 0.00 0.02550 23200.00 10000.00 0.00 0.02667 23400.00 10000.00 0.00 0.02800 23600.00 10000.00 0.00 0.02947 23800.00 10000.00 0.00 0.03091 24000.00 10000.00 0.00 0.03251 24200.00 10000.00 0.00 0.03440 24400.00 10000.00 0.00 0.03630 24600.00 10000.00 0.00 0.03790 24800.00 10000.00 0.00 0.03881 25000.00 10000.00 0.00 0.03890 25200.00 10000.00 0.00 0.03891 25400.00 10000.00 0.00 0.03916 25600.00 10000.00 0.00 0.03933 25400.00 10200.00 0.00 0.03949 25600.00 10200.00 0.00 0.03964 25400.00 10400.00 0.00 0.03984 25600.00 10400.00 0.00 0.04000 25800.00 10400.00 0.00 0.04003 25200.00 10600.00 0.00 0.04004 25400.00 10600.00 0.00 0.04036 25600.00 10600.00 0.00 0.04058 25800.00 10600.00 0.00 0.04065 25200.00 10800.00 0.00 0.04067 25400.00 10800.00 0.00 0.04101 25600.00 10800.00 0.00 0.04127 25800.00 10800.00 0.00 0.04134 25400.00 11000.00 0.00 0.04158 25600.00 11000.00 0.00 0.04184 25800.00 11000.00 0.00 0.04187 25400.00 11200.00 0.00 0.04204 25600.00 11200.00 0.00 0.04229 25400.00 11400.00 0.00 0.04250 25600.00 11400.00 0.00 0.04272 25400.00 11600.00 0.00 0.04296 25600.00 11600.00 0.00 0.04317 25400.00 11800.00 0.00 0.04348 25600.00 11800.00 0.00 0.04369 25400.00 12000.00 0.00 0.04406 25600.00 12000.00 0.00 0.04426 25200.00 12200.00 0.00 0.04433 25400.00 12200.00 0.00 0.04481 25600.00 12200.00 0.00 0.04494 25200.00 12400.00 0.00 0.04508 25400.00 12400.00 0.00 0.04545 25600.00 12400.00 0.00 0.04549 25200.00 12600.00 0.00 0.04562 25400.00 12600.00 0.00 0.04594 25600.00 12600.00 0.00 0.04599 25200.00 12800.00 0.00 0.04619 25400.00 12800.00 0.00 0.04655 25600.00 12800.00 0.00 0.04665 25200.00 13000.00 0.00 0.04675 25400.00 13000.00 0.00 0.04715 25600.00 13000.00 0.00 0.04725 25200.00 13200.00 0.00 0.04742 25400.00 13200.00 0.00 0.04784 25600.00 13200.00 0.00 0.04793 25200.00 13400.00 0.00 0.04813 25400.00 13400.00 0.00 0.04854 25600.00 13400.00 0.00 0.04860 25200.00 13600.00 0.00 0.04885 25400.00 13600.00 0.00 0.04923 25600.00 13600.00 0.00 0.04924 25200.00 13800.00 0.00 0.04962 25400.00 13800.00 0.00 0.04998 25000.00 14000.00 0.00 0.05009 25200.00 14000.00 0.00 0.05047 25400.00 14000.00 0.00 0.05080 25000.00 14200.00 0.00 0.05102 25200.00 14200.00 0.00 0.05137 25400.00 14200.00 0.00 0.05166 25000.00 14400.00 0.00 0.05188 25200.00 14400.00 0.00 0.05220 25400.00 14400.00 0.00 0.05246 24800.00 14600.00 0.00 0.05251 25000.00 14600.00 0.00 0.05278 25200.00 14600.00 0.00 0.05308 25400.00 14600.00 0.00 0.05330 24800.00 14800.00 0.00 0.05332 25000.00 14800.00 0.00 0.05359 25200.00 14800.00 0.00 0.05388 25400.00 14800.00 0.00 0.05408 24800.00 15000.00 0.00 0.05421 25000.00 15000.00 0.00 0.05449 25200.00 15000.00 0.00 0.05478 25400.00 15000.00 0.00 0.05494 24800.00 15200.00 0.00 0.05519 25000.00 15200.00 0.00 0.05546 25200.00 15200.00 0.00 0.05570 25400.00 15200.00 0.00 0.05580 24800.00 15400.00 0.00 0.05610 25000.00 15400.00 0.00 0.05635 25200.00 15400.00 0.00 0.05655 25400.00 15400.00 0.00 0.05661 24800.00 15600.00 0.00 0.05709 25000.00 15600.00 0.00 0.05732 25200.00 15600.00 0.00 0.05748 25400.00 15600.00 0.00 0.05752 24800.00 15800.00 0.00 0.05793 25000.00 15800.00 0.00 0.05813 25200.00 15800.00 0.00 0.05823 25400.00 15800.00 0.00 0.05825 24800.00 16000.00 0.00 0.05869 25000.00 16000.00 0.00 0.05888 25200.00 16000.00 0.00 0.05897 25400.00 16000.00 0.00 0.05900 24800.00 16200.00 0.00 0.05954 25000.00 16200.00 0.00 0.05971 25200.00 16200.00 0.00 0.05979 25400.00 16200.00 0.00 0.05983 24800.00 16400.00 0.00 0.06041 25000.00 16400.00 0.00 0.06054 25200.00 16400.00 0.00 0.06058 24800.00 16600.00 0.00 0.06120 25000.00 16600.00 0.00 0.06129 25200.00 16600.00 0.00 0.06131 24800.00 16800.00 0.00 0.06205 25000.00 16800.00 0.00 0.06212 25200.00 16800.00 0.00 0.06214 24600.00 17000.00 0.00 0.06225 24800.00 17000.00 0.00 0.06302 25000.00 17000.00 0.00 0.06307 25200.00 17000.00 0.00 0.06310 24600.00 17200.00 0.00 0.06322 24800.00 17200.00 0.00 0.06400 25000.00 17200.00 0.00 0.06406 25200.00 17200.00 0.00 0.06411 24600.00 17400.00 0.00 0.06427 24800.00 17400.00 0.00 0.06507 25000.00 17400.00 0.00 0.06515 25200.00 17400.00 0.00 0.06520 24600.00 17600.00 0.00 0.06535 24800.00 17600.00 0.00 0.06615 25000.00 17600.00 0.00 0.06622 25200.00 17600.00 0.00 0.06627 24600.00 17800.00 0.00 0.06659 24800.00 17800.00 0.00 0.06738 25000.00 17800.00 0.00 0.06746 25200.00 17800.00 0.00 0.06752 24600.00 18000.00 0.00 0.06783 24800.00 18000.00 0.00 0.06863 25000.00 18000.00 0.00 0.06871 25200.00 18000.00 0.00 0.06873 24600.00 18200.00 0.00 0.06898 24800.00 18200.00 0.00 0.06978 25000.00 18200.00 0.00 0.06983 24600.00 18400.00 0.00 0.07015 24800.00 18400.00 0.00 0.07095 25000.00 18400.00 0.00 0.07095 24600.00 18600.00 0.00 0.07137 24800.00 18600.00 0.00 0.07211 24600.00 18800.00 0.00 0.07258 24800.00 18800.00 0.00 0.07330 24600.00 19000.00 0.00 0.07380 24800.00 19000.00 0.00 0.07455 24600.00 19200.00 0.00 0.07512 24800.00 19200.00 0.00 0.07588 24600.00 19400.00 0.00 0.07639 24800.00 19400.00 0.00 0.07713 24600.00 19600.00 0.00 0.07780 24800.00 19600.00 0.00 0.07853 24600.00 19800.00 0.00 0.07933 24800.00 19800.00 0.00 0.08005 24600.00 20000.00 0.00 0.08091 24800.00 20000.00 0.00 0.08164 24600.00 20200.00 0.00 0.08248 24800.00 20200.00 0.00 0.08320 24600.00 20400.00 0.00 0.08394 24800.00 20400.00 0.00 0.08464 24600.00 20600.00 0.00 0.08548 24800.00 20600.00 0.00 0.08614 24600.00 20800.00 0.00 0.08697 24800.00 20800.00 0.00 0.08760 24600.00 21000.00 0.00 0.08866 24800.00 21000.00 0.00 0.08925 24600.00 21200.00 0.00 0.09037 24800.00 21200.00 0.00 0.09090 24600.00 21400.00 0.00 0.09204 24800.00 21400.00 0.00 0.09252 24600.00 21600.00 0.00 0.09380 24800.00 21600.00 0.00 0.09426 24600.00 21800.00 0.00 0.09561 24800.00 21800.00 0.00 0.09600 24600.00 22000.00 0.00 0.09751 24800.00 22000.00 0.00 0.09785 24600.00 22200.00 0.00 0.09942 24800.00 22200.00 0.00 0.09977 24600.00 22400.00 0.00 0.10144 24800.00 22400.00 0.00 0.10176 24600.00 22600.00 0.00 0.10334 24800.00 22600.00 0.00 0.10358 24400.00 22800.00 0.00 0.10362 24600.00 22800.00 0.00 0.10528 24800.00 22800.00 0.00 0.10543 24400.00 23000.00 0.00 0.10573 24600.00 23000.00 0.00 0.10736 24800.00 23000.00 0.00 0.10744 24400.00 23200.00 0.00 0.10803 24600.00 23200.00 0.00 0.10958 24400.00 23400.00 0.00 0.11025 24600.00 23400.00 0.00 0.11168 24400.00 23600.00 0.00 0.11226 24600.00 23600.00 0.00 0.11359 24400.00 23800.00 0.00 0.11448 24600.00 23800.00 0.00 0.11566 24400.00 24000.00 0.00 0.11642 24600.00 24000.00 0.00 0.11739 24400.00 24200.00 0.00 0.11828 24600.00 24200.00 0.00 0.11888 24400.00 24400.00 0.00 0.11964 24600.00 24400.00 0.00 0.11979 24400.00 24600.00 0.00 0.11987 24400.00 24600.00 5.00 0.11998 24400.00 24600.00 10.00 0.12009 24400.00 24600.00 15.00 0.12020 24400.00 24600.00 20.00 0.12029 24400.00 24600.00 25.00 0.12037 24400.00 24600.00 30.00 0.12044 24400.00 24600.00 35.00 0.12048 24400.00 24600.00 40.00 0.12050 24400.00 24600.00 45.00 0.12051 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24372.07 24649.83 40.80 0.12056 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/050.ctf NX, NY, NZ: 512 512 1 MODE : real