CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/049.mrc Output diagnostic file name Micrographs_0601/049.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/049.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2532.000 8944.000 Mean, RMS : 4515.035 224.4785 TITLE 1: emi2mrc: 049.emi Sun Jan 05 14:08:49 2014 TITLE 2: kV 300, Mag 78000x, Def 23790, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 88.692, Y 334.559, Z -99.328, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00872 16000.00 10000.00 0.00 -0.00871 16200.00 10000.00 0.00 -0.00821 16400.00 10000.00 0.00 -0.00777 16600.00 10000.00 0.00 -0.00735 16800.00 10000.00 0.00 -0.00682 17000.00 10000.00 0.00 -0.00623 17200.00 10000.00 0.00 -0.00568 17400.00 10000.00 0.00 -0.00506 17600.00 10000.00 0.00 -0.00447 17800.00 10000.00 0.00 -0.00396 18000.00 10000.00 0.00 -0.00327 18200.00 10000.00 0.00 -0.00247 18400.00 10000.00 0.00 -0.00158 18600.00 10000.00 0.00 -0.00081 18800.00 10000.00 0.00 -0.00020 19000.00 10000.00 0.00 0.00052 19200.00 10000.00 0.00 0.00135 19400.00 10000.00 0.00 0.00226 19600.00 10000.00 0.00 0.00321 19800.00 10000.00 0.00 0.00424 20000.00 10000.00 0.00 0.00507 20200.00 10000.00 0.00 0.00580 20400.00 10000.00 0.00 0.00675 20600.00 10000.00 0.00 0.00770 20800.00 10000.00 0.00 0.00867 21000.00 10000.00 0.00 0.00964 21200.00 10000.00 0.00 0.01042 21400.00 10000.00 0.00 0.01129 21600.00 10000.00 0.00 0.01228 21800.00 10000.00 0.00 0.01319 22000.00 10000.00 0.00 0.01410 22200.00 10000.00 0.00 0.01512 22400.00 10000.00 0.00 0.01626 22600.00 10000.00 0.00 0.01747 22800.00 10000.00 0.00 0.01857 23000.00 10000.00 0.00 0.01968 23200.00 10000.00 0.00 0.02095 23400.00 10000.00 0.00 0.02227 23600.00 10000.00 0.00 0.02348 23800.00 10000.00 0.00 0.02475 24000.00 10000.00 0.00 0.02622 24200.00 10000.00 0.00 0.02776 24400.00 10000.00 0.00 0.02942 24600.00 10000.00 0.00 0.03116 24800.00 10000.00 0.00 0.03289 25000.00 10000.00 0.00 0.03430 25200.00 10000.00 0.00 0.03511 25400.00 10000.00 0.00 0.03549 25600.00 10000.00 0.00 0.03575 25800.00 10000.00 0.00 0.03601 26000.00 10000.00 0.00 0.03609 25800.00 10200.00 0.00 0.03632 26000.00 10200.00 0.00 0.03634 25600.00 10400.00 0.00 0.03655 25800.00 10400.00 0.00 0.03675 25400.00 10600.00 0.00 0.03687 25600.00 10600.00 0.00 0.03713 25800.00 10600.00 0.00 0.03729 25400.00 10800.00 0.00 0.03745 25600.00 10800.00 0.00 0.03765 25800.00 10800.00 0.00 0.03779 26000.00 10800.00 0.00 0.03779 25400.00 11000.00 0.00 0.03795 25600.00 11000.00 0.00 0.03813 25800.00 11000.00 0.00 0.03829 26000.00 11000.00 0.00 0.03833 25400.00 11200.00 0.00 0.03847 25600.00 11200.00 0.00 0.03868 25800.00 11200.00 0.00 0.03888 26000.00 11200.00 0.00 0.03892 25400.00 11400.00 0.00 0.03896 25600.00 11400.00 0.00 0.03918 25800.00 11400.00 0.00 0.03937 25400.00 11600.00 0.00 0.03938 25600.00 11600.00 0.00 0.03959 25800.00 11600.00 0.00 0.03974 25400.00 11800.00 0.00 0.03980 25600.00 11800.00 0.00 0.04002 25800.00 11800.00 0.00 0.04015 25400.00 12000.00 0.00 0.04033 25600.00 12000.00 0.00 0.04055 25800.00 12000.00 0.00 0.04066 25400.00 12200.00 0.00 0.04103 25600.00 12200.00 0.00 0.04123 25800.00 12200.00 0.00 0.04130 25400.00 12400.00 0.00 0.04165 25600.00 12400.00 0.00 0.04179 25800.00 12400.00 0.00 0.04185 25400.00 12600.00 0.00 0.04213 25600.00 12600.00 0.00 0.04227 25800.00 12600.00 0.00 0.04236 25400.00 12800.00 0.00 0.04261 25600.00 12800.00 0.00 0.04279 25800.00 12800.00 0.00 0.04293 25400.00 13000.00 0.00 0.04305 25600.00 13000.00 0.00 0.04331 25800.00 13000.00 0.00 0.04346 25400.00 13200.00 0.00 0.04367 25600.00 13200.00 0.00 0.04395 25800.00 13200.00 0.00 0.04404 25400.00 13400.00 0.00 0.04436 25600.00 13400.00 0.00 0.04459 25800.00 13400.00 0.00 0.04460 25400.00 13600.00 0.00 0.04498 25600.00 13600.00 0.00 0.04515 25200.00 13800.00 0.00 0.04517 25400.00 13800.00 0.00 0.04553 25600.00 13800.00 0.00 0.04565 25200.00 14000.00 0.00 0.04565 25400.00 14000.00 0.00 0.04599 25600.00 14000.00 0.00 0.04609 25200.00 14200.00 0.00 0.04615 25400.00 14200.00 0.00 0.04651 25600.00 14200.00 0.00 0.04663 25200.00 14400.00 0.00 0.04676 25400.00 14400.00 0.00 0.04712 25600.00 14400.00 0.00 0.04724 25200.00 14600.00 0.00 0.04736 25400.00 14600.00 0.00 0.04771 25600.00 14600.00 0.00 0.04781 25200.00 14800.00 0.00 0.04805 25400.00 14800.00 0.00 0.04840 25600.00 14800.00 0.00 0.04850 25200.00 15000.00 0.00 0.04893 25400.00 15000.00 0.00 0.04925 25600.00 15000.00 0.00 0.04933 25200.00 15200.00 0.00 0.04974 25400.00 15200.00 0.00 0.05003 25600.00 15200.00 0.00 0.05008 25200.00 15400.00 0.00 0.05058 25400.00 15400.00 0.00 0.05087 25600.00 15400.00 0.00 0.05092 25200.00 15600.00 0.00 0.05146 25400.00 15600.00 0.00 0.05173 25600.00 15600.00 0.00 0.05176 25200.00 15800.00 0.00 0.05238 25400.00 15800.00 0.00 0.05263 25600.00 15800.00 0.00 0.05265 25200.00 16000.00 0.00 0.05347 25400.00 16000.00 0.00 0.05371 25600.00 16000.00 0.00 0.05373 25200.00 16200.00 0.00 0.05439 25400.00 16200.00 0.00 0.05461 25600.00 16200.00 0.00 0.05463 25200.00 16400.00 0.00 0.05526 25400.00 16400.00 0.00 0.05548 25600.00 16400.00 0.00 0.05552 25200.00 16600.00 0.00 0.05622 25400.00 16600.00 0.00 0.05646 25600.00 16600.00 0.00 0.05653 25200.00 16800.00 0.00 0.05725 25400.00 16800.00 0.00 0.05751 25600.00 16800.00 0.00 0.05757 25200.00 17000.00 0.00 0.05828 25400.00 17000.00 0.00 0.05853 25600.00 17000.00 0.00 0.05858 25200.00 17200.00 0.00 0.05930 25400.00 17200.00 0.00 0.05956 25600.00 17200.00 0.00 0.05959 25000.00 17400.00 0.00 0.05960 25200.00 17400.00 0.00 0.06041 25400.00 17400.00 0.00 0.06068 25600.00 17400.00 0.00 0.06070 25200.00 17600.00 0.00 0.06144 25400.00 17600.00 0.00 0.06174 25600.00 17600.00 0.00 0.06174 25200.00 17800.00 0.00 0.06261 25400.00 17800.00 0.00 0.06296 25000.00 18000.00 0.00 0.06298 25200.00 18000.00 0.00 0.06397 25400.00 18000.00 0.00 0.06430 25000.00 18200.00 0.00 0.06433 25200.00 18200.00 0.00 0.06534 25400.00 18200.00 0.00 0.06559 25000.00 18400.00 0.00 0.06571 25200.00 18400.00 0.00 0.06671 25400.00 18400.00 0.00 0.06688 25000.00 18600.00 0.00 0.06721 25200.00 18600.00 0.00 0.06816 25400.00 18600.00 0.00 0.06826 25000.00 18800.00 0.00 0.06865 25200.00 18800.00 0.00 0.06954 25400.00 18800.00 0.00 0.06961 25000.00 19000.00 0.00 0.06990 25200.00 19000.00 0.00 0.07077 25400.00 19000.00 0.00 0.07086 25000.00 19200.00 0.00 0.07120 25200.00 19200.00 0.00 0.07209 25400.00 19200.00 0.00 0.07219 25000.00 19400.00 0.00 0.07256 25200.00 19400.00 0.00 0.07347 25400.00 19400.00 0.00 0.07353 25000.00 19600.00 0.00 0.07407 25200.00 19600.00 0.00 0.07496 25400.00 19600.00 0.00 0.07497 25000.00 19800.00 0.00 0.07559 25200.00 19800.00 0.00 0.07646 25000.00 20000.00 0.00 0.07726 25200.00 20000.00 0.00 0.07811 25000.00 20200.00 0.00 0.07895 25200.00 20200.00 0.00 0.07974 25000.00 20400.00 0.00 0.08048 25200.00 20400.00 0.00 0.08123 25000.00 20600.00 0.00 0.08213 25200.00 20600.00 0.00 0.08282 25000.00 20800.00 0.00 0.08361 25200.00 20800.00 0.00 0.08422 25000.00 21000.00 0.00 0.08504 25200.00 21000.00 0.00 0.08563 25000.00 21200.00 0.00 0.08656 25200.00 21200.00 0.00 0.08715 25000.00 21400.00 0.00 0.08824 25200.00 21400.00 0.00 0.08881 25000.00 21600.00 0.00 0.09000 25200.00 21600.00 0.00 0.09050 25000.00 21800.00 0.00 0.09175 25200.00 21800.00 0.00 0.09218 25000.00 22000.00 0.00 0.09359 25200.00 22000.00 0.00 0.09397 25000.00 22200.00 0.00 0.09544 25200.00 22200.00 0.00 0.09581 25000.00 22400.00 0.00 0.09739 25200.00 22400.00 0.00 0.09772 25000.00 22600.00 0.00 0.09919 25200.00 22600.00 0.00 0.09945 24800.00 22800.00 0.00 0.09954 25000.00 22800.00 0.00 0.10117 25200.00 22800.00 0.00 0.10137 24800.00 23000.00 0.00 0.10174 25000.00 23000.00 0.00 0.10335 25200.00 23000.00 0.00 0.10347 24800.00 23200.00 0.00 0.10396 25000.00 23200.00 0.00 0.10552 24800.00 23400.00 0.00 0.10619 25000.00 23400.00 0.00 0.10763 24800.00 23600.00 0.00 0.10839 25000.00 23600.00 0.00 0.10972 24800.00 23800.00 0.00 0.11077 25000.00 23800.00 0.00 0.11195 24800.00 24000.00 0.00 0.11301 25000.00 24000.00 0.00 0.11399 24800.00 24200.00 0.00 0.11529 25000.00 24200.00 0.00 0.11604 24800.00 24400.00 0.00 0.11736 25000.00 24400.00 0.00 0.11787 24800.00 24600.00 0.00 0.11952 25000.00 24600.00 0.00 0.11963 24800.00 24800.00 0.00 0.12120 24800.00 25000.00 20.00 0.12124 24800.00 25000.00 25.00 0.12131 24800.00 25000.00 30.00 0.12136 24800.00 25000.00 35.00 0.12139 24800.00 25000.00 40.00 0.12141 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24773.45 24929.97 43.07 0.12156 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/049.ctf NX, NY, NZ: 512 512 1 MODE : real