CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/048.mrc Output diagnostic file name Micrographs_0601/048.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/048.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2384.000 7796.000 Mean, RMS : 4510.254 223.6416 TITLE 1: emi2mrc: 048.emi Sun Jan 05 14:07:59 2014 TITLE 2: kV 300, Mag 78000x, Def 23790, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 86.163, Y 334.923, Z -99.328, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01092 16400.00 10000.00 0.00 -0.01074 16600.00 10000.00 0.00 -0.01026 16800.00 10000.00 0.00 -0.00984 17000.00 10000.00 0.00 -0.00939 17200.00 10000.00 0.00 -0.00897 17400.00 10000.00 0.00 -0.00856 17600.00 10000.00 0.00 -0.00799 17800.00 10000.00 0.00 -0.00740 18000.00 10000.00 0.00 -0.00689 18200.00 10000.00 0.00 -0.00631 18400.00 10000.00 0.00 -0.00558 18600.00 10000.00 0.00 -0.00478 18800.00 10000.00 0.00 -0.00400 19000.00 10000.00 0.00 -0.00319 19200.00 10000.00 0.00 -0.00235 19400.00 10000.00 0.00 -0.00155 19600.00 10000.00 0.00 -0.00073 19800.00 10000.00 0.00 0.00002 20000.00 10000.00 0.00 0.00075 20200.00 10000.00 0.00 0.00163 20400.00 10000.00 0.00 0.00252 20600.00 10000.00 0.00 0.00344 20800.00 10000.00 0.00 0.00444 21000.00 10000.00 0.00 0.00529 21200.00 10000.00 0.00 0.00611 21400.00 10000.00 0.00 0.00706 21600.00 10000.00 0.00 0.00788 21800.00 10000.00 0.00 0.00861 22000.00 10000.00 0.00 0.00939 22200.00 10000.00 0.00 0.01017 22400.00 10000.00 0.00 0.01109 22600.00 10000.00 0.00 0.01214 22800.00 10000.00 0.00 0.01312 23000.00 10000.00 0.00 0.01417 23200.00 10000.00 0.00 0.01537 23400.00 10000.00 0.00 0.01657 23600.00 10000.00 0.00 0.01761 23800.00 10000.00 0.00 0.01876 24000.00 10000.00 0.00 0.02008 24200.00 10000.00 0.00 0.02148 24400.00 10000.00 0.00 0.02290 24600.00 10000.00 0.00 0.02427 24800.00 10000.00 0.00 0.02585 25000.00 10000.00 0.00 0.02752 25200.00 10000.00 0.00 0.02925 25400.00 10000.00 0.00 0.03121 25600.00 10000.00 0.00 0.03316 25800.00 10000.00 0.00 0.03469 26000.00 10000.00 0.00 0.03555 26200.00 10000.00 0.00 0.03607 26400.00 10000.00 0.00 0.03643 26400.00 10200.00 0.00 0.03669 26200.00 10400.00 0.00 0.03671 26400.00 10400.00 0.00 0.03694 26200.00 10600.00 0.00 0.03705 26400.00 10600.00 0.00 0.03726 26200.00 10800.00 0.00 0.03737 26400.00 10800.00 0.00 0.03761 26200.00 11000.00 0.00 0.03771 26400.00 11000.00 0.00 0.03795 26200.00 11200.00 0.00 0.03800 26400.00 11200.00 0.00 0.03822 26200.00 11400.00 0.00 0.03834 26400.00 11400.00 0.00 0.03854 26200.00 11600.00 0.00 0.03873 26400.00 11600.00 0.00 0.03892 26200.00 11800.00 0.00 0.03912 26400.00 11800.00 0.00 0.03930 26200.00 12000.00 0.00 0.03947 26400.00 12000.00 0.00 0.03963 26200.00 12200.00 0.00 0.03983 26400.00 12200.00 0.00 0.03996 26200.00 12400.00 0.00 0.04018 26400.00 12400.00 0.00 0.04030 26200.00 12600.00 0.00 0.04050 26400.00 12600.00 0.00 0.04063 26200.00 12800.00 0.00 0.04085 26400.00 12800.00 0.00 0.04098 26200.00 13000.00 0.00 0.04125 26400.00 13000.00 0.00 0.04139 26200.00 13200.00 0.00 0.04184 26400.00 13200.00 0.00 0.04197 26200.00 13400.00 0.00 0.04237 26400.00 13400.00 0.00 0.04245 26000.00 13600.00 0.00 0.04251 26200.00 13600.00 0.00 0.04290 26400.00 13600.00 0.00 0.04295 26000.00 13800.00 0.00 0.04320 26200.00 13800.00 0.00 0.04356 26400.00 13800.00 0.00 0.04359 26000.00 14000.00 0.00 0.04386 26200.00 14000.00 0.00 0.04420 26400.00 14000.00 0.00 0.04422 26000.00 14200.00 0.00 0.04452 26200.00 14200.00 0.00 0.04485 26400.00 14200.00 0.00 0.04486 26000.00 14400.00 0.00 0.04510 26200.00 14400.00 0.00 0.04540 26000.00 14600.00 0.00 0.04561 26200.00 14600.00 0.00 0.04594 26000.00 14800.00 0.00 0.04617 26200.00 14800.00 0.00 0.04651 26000.00 15000.00 0.00 0.04685 26200.00 15000.00 0.00 0.04714 26000.00 15200.00 0.00 0.04751 26200.00 15200.00 0.00 0.04775 26000.00 15400.00 0.00 0.04816 26200.00 15400.00 0.00 0.04840 26400.00 15400.00 0.00 0.04842 26000.00 15600.00 0.00 0.04885 26200.00 15600.00 0.00 0.04911 26400.00 15600.00 0.00 0.04915 26000.00 15800.00 0.00 0.04948 26200.00 15800.00 0.00 0.04977 26400.00 15800.00 0.00 0.04983 26000.00 16000.00 0.00 0.05017 26200.00 16000.00 0.00 0.05051 26400.00 16000.00 0.00 0.05058 26000.00 16200.00 0.00 0.05101 26200.00 16200.00 0.00 0.05136 26400.00 16200.00 0.00 0.05141 26000.00 16400.00 0.00 0.05199 26200.00 16400.00 0.00 0.05231 26000.00 16600.00 0.00 0.05286 26200.00 16600.00 0.00 0.05317 26000.00 16800.00 0.00 0.05375 26200.00 16800.00 0.00 0.05406 26000.00 17000.00 0.00 0.05474 26200.00 17000.00 0.00 0.05503 26000.00 17200.00 0.00 0.05570 26200.00 17200.00 0.00 0.05596 26000.00 17400.00 0.00 0.05664 26200.00 17400.00 0.00 0.05685 26000.00 17600.00 0.00 0.05742 26200.00 17600.00 0.00 0.05761 26000.00 17800.00 0.00 0.05836 26200.00 17800.00 0.00 0.05859 25800.00 18000.00 0.00 0.05871 26000.00 18000.00 0.00 0.05952 26200.00 18000.00 0.00 0.05978 25800.00 18200.00 0.00 0.05999 26000.00 18200.00 0.00 0.06078 26200.00 18200.00 0.00 0.06099 25800.00 18400.00 0.00 0.06132 26000.00 18400.00 0.00 0.06206 26200.00 18400.00 0.00 0.06220 25800.00 18600.00 0.00 0.06257 26000.00 18600.00 0.00 0.06327 26200.00 18600.00 0.00 0.06341 25800.00 18800.00 0.00 0.06384 26000.00 18800.00 0.00 0.06453 26200.00 18800.00 0.00 0.06469 25800.00 19000.00 0.00 0.06501 26000.00 19000.00 0.00 0.06565 26200.00 19000.00 0.00 0.06577 25800.00 19200.00 0.00 0.06611 26000.00 19200.00 0.00 0.06673 26200.00 19200.00 0.00 0.06683 25800.00 19400.00 0.00 0.06727 26000.00 19400.00 0.00 0.06790 26200.00 19400.00 0.00 0.06801 25800.00 19600.00 0.00 0.06855 26000.00 19600.00 0.00 0.06917 26200.00 19600.00 0.00 0.06926 25800.00 19800.00 0.00 0.06984 26000.00 19800.00 0.00 0.07045 26200.00 19800.00 0.00 0.07051 25800.00 20000.00 0.00 0.07110 26000.00 20000.00 0.00 0.07169 26200.00 20000.00 0.00 0.07170 25800.00 20200.00 0.00 0.07228 26000.00 20200.00 0.00 0.07285 25800.00 20400.00 0.00 0.07346 26000.00 20400.00 0.00 0.07404 25800.00 20600.00 0.00 0.07476 26000.00 20600.00 0.00 0.07533 25800.00 20800.00 0.00 0.07613 26000.00 20800.00 0.00 0.07669 25800.00 21000.00 0.00 0.07771 26000.00 21000.00 0.00 0.07825 25800.00 21200.00 0.00 0.07921 26000.00 21200.00 0.00 0.07975 25800.00 21400.00 0.00 0.08076 26000.00 21400.00 0.00 0.08128 25800.00 21600.00 0.00 0.08227 26000.00 21600.00 0.00 0.08278 25800.00 21800.00 0.00 0.08366 26000.00 21800.00 0.00 0.08414 25800.00 22000.00 0.00 0.08524 26000.00 22000.00 0.00 0.08569 25800.00 22200.00 0.00 0.08677 26000.00 22200.00 0.00 0.08718 25800.00 22400.00 0.00 0.08844 26000.00 22400.00 0.00 0.08880 25800.00 22600.00 0.00 0.09019 26000.00 22600.00 0.00 0.09048 25800.00 22800.00 0.00 0.09200 26000.00 22800.00 0.00 0.09225 25600.00 23000.00 0.00 0.09234 25800.00 23000.00 0.00 0.09389 26000.00 23000.00 0.00 0.09409 25600.00 23200.00 0.00 0.09424 25800.00 23200.00 0.00 0.09577 26000.00 23200.00 0.00 0.09591 25600.00 23400.00 0.00 0.09629 25800.00 23400.00 0.00 0.09778 26000.00 23400.00 0.00 0.09782 25600.00 23600.00 0.00 0.09832 25800.00 23600.00 0.00 0.09975 25600.00 23800.00 0.00 0.10034 25800.00 23800.00 0.00 0.10171 25600.00 24000.00 0.00 0.10221 25800.00 24000.00 0.00 0.10354 25600.00 24200.00 0.00 0.10412 25800.00 24200.00 0.00 0.10537 25600.00 24400.00 0.00 0.10615 25800.00 24400.00 0.00 0.10724 25600.00 24600.00 0.00 0.10819 25800.00 24600.00 0.00 0.10913 25600.00 24800.00 0.00 0.11012 25800.00 24800.00 0.00 0.11093 25600.00 25000.00 0.00 0.11202 25800.00 25000.00 0.00 0.11264 25600.00 25200.00 0.00 0.11391 25800.00 25200.00 0.00 0.11428 25600.00 25400.00 0.00 0.11551 25800.00 25400.00 0.00 0.11558 25600.00 25600.00 0.00 0.11707 25600.00 25800.00 0.00 0.11719 25600.00 25800.00 5.00 0.11729 25600.00 25800.00 10.00 0.11738 25600.00 25800.00 15.00 0.11746 25600.00 25800.00 20.00 0.11753 25600.00 25800.00 25.00 0.11758 25600.00 25800.00 30.00 0.11760 25600.00 25800.00 35.00 0.11761 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25588.93 25752.38 34.93 0.11770 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/048.ctf NX, NY, NZ: 512 512 1 MODE : real