CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/042.mrc Output diagnostic file name Micrographs_0601/042.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/042.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2404.000 8076.000 Mean, RMS : 4523.703 224.2516 TITLE 1: emi2mrc: 042.emi Sun Jan 05 14:02:10 2014 TITLE 2: kV 300, Mag 78000x, Def 23790, Spot 4, Int 55.10%, Obj 88.05%, Dif 73.67% TITLE 3: X 90.893, Y 331.894, Z -99.328, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00999 10200.00 10000.00 0.00 -0.00991 10400.00 10000.00 0.00 -0.00985 11400.00 10000.00 0.00 -0.00977 11600.00 10000.00 0.00 -0.00962 11800.00 10000.00 0.00 -0.00938 12000.00 10000.00 0.00 -0.00915 12200.00 10000.00 0.00 -0.00903 12400.00 10000.00 0.00 -0.00894 12600.00 10000.00 0.00 -0.00864 12800.00 10000.00 0.00 -0.00831 13000.00 10000.00 0.00 -0.00811 13200.00 10000.00 0.00 -0.00800 13400.00 10000.00 0.00 -0.00786 13600.00 10000.00 0.00 -0.00770 13800.00 10000.00 0.00 -0.00755 14000.00 10000.00 0.00 -0.00729 14200.00 10000.00 0.00 -0.00696 14400.00 10000.00 0.00 -0.00665 14600.00 10000.00 0.00 -0.00635 14800.00 10000.00 0.00 -0.00595 15000.00 10000.00 0.00 -0.00541 15200.00 10000.00 0.00 -0.00486 15400.00 10000.00 0.00 -0.00440 15600.00 10000.00 0.00 -0.00397 15800.00 10000.00 0.00 -0.00348 16000.00 10000.00 0.00 -0.00304 16200.00 10000.00 0.00 -0.00257 16400.00 10000.00 0.00 -0.00199 16600.00 10000.00 0.00 -0.00134 16800.00 10000.00 0.00 -0.00061 17000.00 10000.00 0.00 0.00009 17200.00 10000.00 0.00 0.00072 17400.00 10000.00 0.00 0.00138 17600.00 10000.00 0.00 0.00209 17800.00 10000.00 0.00 0.00288 18000.00 10000.00 0.00 0.00372 18200.00 10000.00 0.00 0.00457 18400.00 10000.00 0.00 0.00551 18600.00 10000.00 0.00 0.00648 18800.00 10000.00 0.00 0.00739 19000.00 10000.00 0.00 0.00823 19200.00 10000.00 0.00 0.00904 19400.00 10000.00 0.00 0.00984 19600.00 10000.00 0.00 0.01065 19800.00 10000.00 0.00 0.01149 20000.00 10000.00 0.00 0.01244 20200.00 10000.00 0.00 0.01352 20400.00 10000.00 0.00 0.01453 20600.00 10000.00 0.00 0.01561 20800.00 10000.00 0.00 0.01672 21000.00 10000.00 0.00 0.01765 21200.00 10000.00 0.00 0.01871 21400.00 10000.00 0.00 0.01994 21600.00 10000.00 0.00 0.02118 21800.00 10000.00 0.00 0.02236 22000.00 10000.00 0.00 0.02354 22200.00 10000.00 0.00 0.02477 22400.00 10000.00 0.00 0.02596 22600.00 10000.00 0.00 0.02727 22800.00 10000.00 0.00 0.02870 23000.00 10000.00 0.00 0.03018 23200.00 10000.00 0.00 0.03169 23400.00 10000.00 0.00 0.03325 23600.00 10000.00 0.00 0.03488 23800.00 10000.00 0.00 0.03654 24000.00 10000.00 0.00 0.03807 24200.00 10000.00 0.00 0.03924 24400.00 10000.00 0.00 0.04002 24600.00 10000.00 0.00 0.04060 24800.00 10000.00 0.00 0.04102 25000.00 10000.00 0.00 0.04126 25200.00 10000.00 0.00 0.04132 25000.00 10200.00 0.00 0.04146 25200.00 10200.00 0.00 0.04150 25000.00 10400.00 0.00 0.04167 25200.00 10400.00 0.00 0.04171 24800.00 10600.00 0.00 0.04183 25000.00 10600.00 0.00 0.04202 25200.00 10600.00 0.00 0.04205 24800.00 10800.00 0.00 0.04221 25000.00 10800.00 0.00 0.04240 25200.00 10800.00 0.00 0.04240 24800.00 11000.00 0.00 0.04262 25000.00 11000.00 0.00 0.04277 24800.00 11200.00 0.00 0.04297 25000.00 11200.00 0.00 0.04308 24800.00 11400.00 0.00 0.04334 25000.00 11400.00 0.00 0.04344 24800.00 11600.00 0.00 0.04368 25000.00 11600.00 0.00 0.04377 24600.00 11800.00 0.00 0.04384 24800.00 11800.00 0.00 0.04411 25000.00 11800.00 0.00 0.04424 25200.00 11800.00 0.00 0.04427 24600.00 12000.00 0.00 0.04432 24800.00 12000.00 0.00 0.04462 25000.00 12000.00 0.00 0.04479 25200.00 12000.00 0.00 0.04485 24800.00 12200.00 0.00 0.04514 25000.00 12200.00 0.00 0.04530 25200.00 12200.00 0.00 0.04534 24600.00 12400.00 0.00 0.04537 24800.00 12400.00 0.00 0.04568 25000.00 12400.00 0.00 0.04581 24600.00 12600.00 0.00 0.04592 24800.00 12600.00 0.00 0.04619 25000.00 12600.00 0.00 0.04630 24600.00 12800.00 0.00 0.04652 24800.00 12800.00 0.00 0.04676 25000.00 12800.00 0.00 0.04684 24600.00 13000.00 0.00 0.04703 24800.00 13000.00 0.00 0.04724 25000.00 13000.00 0.00 0.04733 24600.00 13200.00 0.00 0.04756 24800.00 13200.00 0.00 0.04779 25000.00 13200.00 0.00 0.04790 24600.00 13400.00 0.00 0.04820 24800.00 13400.00 0.00 0.04845 25000.00 13400.00 0.00 0.04856 24600.00 13600.00 0.00 0.04895 24800.00 13600.00 0.00 0.04920 25000.00 13600.00 0.00 0.04931 24600.00 13800.00 0.00 0.04973 24800.00 13800.00 0.00 0.04999 25000.00 13800.00 0.00 0.05012 25200.00 13800.00 0.00 0.05014 24600.00 14000.00 0.00 0.05045 24800.00 14000.00 0.00 0.05075 25000.00 14000.00 0.00 0.05090 24600.00 14200.00 0.00 0.05132 24800.00 14200.00 0.00 0.05164 25000.00 14200.00 0.00 0.05177 24400.00 14400.00 0.00 0.05184 24600.00 14400.00 0.00 0.05234 24800.00 14400.00 0.00 0.05264 25000.00 14400.00 0.00 0.05271 24400.00 14600.00 0.00 0.05288 24600.00 14600.00 0.00 0.05336 24800.00 14600.00 0.00 0.05360 25000.00 14600.00 0.00 0.05363 24400.00 14800.00 0.00 0.05378 24600.00 14800.00 0.00 0.05422 24800.00 14800.00 0.00 0.05443 25000.00 14800.00 0.00 0.05443 24400.00 15000.00 0.00 0.05461 24600.00 15000.00 0.00 0.05503 24800.00 15000.00 0.00 0.05521 24400.00 15200.00 0.00 0.05543 24600.00 15200.00 0.00 0.05584 24800.00 15200.00 0.00 0.05601 25000.00 15200.00 0.00 0.05601 24400.00 15400.00 0.00 0.05643 24600.00 15400.00 0.00 0.05682 24800.00 15400.00 0.00 0.05699 25000.00 15400.00 0.00 0.05701 24400.00 15600.00 0.00 0.05748 24600.00 15600.00 0.00 0.05785 24800.00 15600.00 0.00 0.05802 25000.00 15600.00 0.00 0.05806 24400.00 15800.00 0.00 0.05839 24600.00 15800.00 0.00 0.05877 24800.00 15800.00 0.00 0.05897 25000.00 15800.00 0.00 0.05903 24400.00 16000.00 0.00 0.05943 24600.00 16000.00 0.00 0.05982 24800.00 16000.00 0.00 0.06002 25000.00 16000.00 0.00 0.06006 24400.00 16200.00 0.00 0.06050 24600.00 16200.00 0.00 0.06087 24800.00 16200.00 0.00 0.06103 25000.00 16200.00 0.00 0.06104 24400.00 16400.00 0.00 0.06162 24600.00 16400.00 0.00 0.06195 24800.00 16400.00 0.00 0.06209 24400.00 16600.00 0.00 0.06263 24600.00 16600.00 0.00 0.06291 24800.00 16600.00 0.00 0.06304 24400.00 16800.00 0.00 0.06359 24600.00 16800.00 0.00 0.06386 24800.00 16800.00 0.00 0.06397 24400.00 17000.00 0.00 0.06472 24600.00 17000.00 0.00 0.06497 24800.00 17000.00 0.00 0.06510 24400.00 17200.00 0.00 0.06591 24600.00 17200.00 0.00 0.06617 24800.00 17200.00 0.00 0.06630 24200.00 17400.00 0.00 0.06630 24400.00 17400.00 0.00 0.06717 24600.00 17400.00 0.00 0.06744 24800.00 17400.00 0.00 0.06756 24200.00 17600.00 0.00 0.06760 24400.00 17600.00 0.00 0.06848 24600.00 17600.00 0.00 0.06873 24800.00 17600.00 0.00 0.06882 24200.00 17800.00 0.00 0.06900 24400.00 17800.00 0.00 0.06986 24600.00 17800.00 0.00 0.07008 24800.00 17800.00 0.00 0.07012 24200.00 18000.00 0.00 0.07030 24400.00 18000.00 0.00 0.07112 24600.00 18000.00 0.00 0.07132 24800.00 18000.00 0.00 0.07134 24200.00 18200.00 0.00 0.07168 24400.00 18200.00 0.00 0.07247 24600.00 18200.00 0.00 0.07266 24800.00 18200.00 0.00 0.07266 24200.00 18400.00 0.00 0.07312 24400.00 18400.00 0.00 0.07390 24600.00 18400.00 0.00 0.07409 24200.00 18600.00 0.00 0.07456 24400.00 18600.00 0.00 0.07536 24600.00 18600.00 0.00 0.07556 24200.00 18800.00 0.00 0.07614 24400.00 18800.00 0.00 0.07695 24600.00 18800.00 0.00 0.07713 24200.00 19000.00 0.00 0.07763 24400.00 19000.00 0.00 0.07843 24600.00 19000.00 0.00 0.07857 24200.00 19200.00 0.00 0.07915 24400.00 19200.00 0.00 0.07995 24600.00 19200.00 0.00 0.08003 24200.00 19400.00 0.00 0.08068 24400.00 19400.00 0.00 0.08144 24600.00 19400.00 0.00 0.08146 24200.00 19600.00 0.00 0.08226 24400.00 19600.00 0.00 0.08296 24200.00 19800.00 0.00 0.08376 24400.00 19800.00 0.00 0.08442 24200.00 20000.00 0.00 0.08515 24400.00 20000.00 0.00 0.08576 24200.00 20200.00 0.00 0.08651 24400.00 20200.00 0.00 0.08706 24200.00 20400.00 0.00 0.08787 24400.00 20400.00 0.00 0.08840 24200.00 20600.00 0.00 0.08942 24400.00 20600.00 0.00 0.08992 24200.00 20800.00 0.00 0.09094 24400.00 20800.00 0.00 0.09141 24200.00 21000.00 0.00 0.09254 24400.00 21000.00 0.00 0.09297 24200.00 21200.00 0.00 0.09413 24400.00 21200.00 0.00 0.09458 24200.00 21400.00 0.00 0.09586 24400.00 21400.00 0.00 0.09635 24200.00 21600.00 0.00 0.09768 24400.00 21600.00 0.00 0.09817 24200.00 21800.00 0.00 0.09952 24400.00 21800.00 0.00 0.09996 24000.00 22000.00 0.00 0.09997 24200.00 22000.00 0.00 0.10148 24400.00 22000.00 0.00 0.10185 24000.00 22200.00 0.00 0.10199 24200.00 22200.00 0.00 0.10349 24400.00 22200.00 0.00 0.10376 24000.00 22400.00 0.00 0.10421 24200.00 22400.00 0.00 0.10566 24400.00 22400.00 0.00 0.10582 24000.00 22600.00 0.00 0.10620 24200.00 22600.00 0.00 0.10760 24400.00 22600.00 0.00 0.10765 24000.00 22800.00 0.00 0.10829 24200.00 22800.00 0.00 0.10962 24000.00 23000.00 0.00 0.11032 24200.00 23000.00 0.00 0.11156 24000.00 23200.00 0.00 0.11217 24200.00 23200.00 0.00 0.11332 24000.00 23400.00 0.00 0.11412 24200.00 23400.00 0.00 0.11516 24000.00 23600.00 0.00 0.11595 24200.00 23600.00 0.00 0.11682 24000.00 23800.00 0.00 0.11799 24200.00 23800.00 0.00 0.11856 24000.00 24000.00 0.00 0.11959 24200.00 24000.00 0.00 0.11987 24000.00 24200.00 0.00 0.12052 24000.00 24200.00 5.00 0.12060 24000.00 24200.00 10.00 0.12066 24000.00 24200.00 15.00 0.12071 24000.00 24200.00 20.00 0.12074 24000.00 24200.00 25.00 0.12076 24000.00 24200.00 30.00 0.12076 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24042.49 24243.07 26.55 0.12086 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/042.ctf NX, NY, NZ: 512 512 1 MODE : real